Aims: Distillers dried grains are the nutrient rich co-product of dry-milled ethanol production. The present study aimed to prove that the nutritional composition of distillers dried grain from a crude hydrolysate of rice husk fermented by co-cultures of Saccharomyces cerevisiae with Candida tropicalis difference from unfermented crude rice husk hydrolysate and mono-cultured S. cerevisiae or C. tropicalis. Methodology and results: The effects of mono- and co-cultures S. cerevisiae with C. tropicalis on the nutrient compositions of distillers dried grain were investigated. The crude rice husk hydrolysate in distilled water contained molasses, urea, sodium nitrate, ammonium nitrate, potassium phosphate and magnesium sulfate heptahydrate were fermented by mono- and co-cultures of S. cerevisiae with C. tropicalis for 7 days at 28-30 °C and stored with a relative humidity of 60-70% in the dark. A mono- and a co-culture fermentation of S. cerevisiae and C. tropicalis increased the crude protein, crude fat, crude fibres, ash, and calcium contents of the rice husk feedstock and decreased the metabolic energy reducing sugars. Conclusion, significance and impact of study Some nutrient components of the DDG crude rice husk hydrolysate performed higher than the non-fermentation of rice husks. The finding of this study will serve as a basic reference for future studies to utilize by-product of ethanol production from rice husks for animal feed formulation.