Objective To investigate the effects and mechanisms of LINC00657 on oxidative glu-cose deprivation(OGD)-induced injury in mouse hippocampal neurons.Methods Mouse hippocam-pal neuron cell line HT22 was given OGD treatment to establish an injury model,with normally cul-tured HT22 cells as controls.The si-NC,si-LINC00657,microRNA(miR)-NC,and miR-224-3p mimics were transfected into HT22 cells,followed by OGD treatment.Co-transfection of si-LINC00657 and anti-miR-NC,or co-transfection of si-LINC00657 and anti-miR-224-3p,was performed in HT22 cells before OGD treatment.Real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of LINC00657 and miR-224-3p.CCK-8 assay and flow cytometry were used to detect cell viability and apoptosis rate,respectively.Kits were used to detect the activities of lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and the level of malondialdehyde(MDA).Dual-luciferase reporter gene assay was used to detect the effect of miR-224-3p overexpression on the luciferase activity of wild-type LINC00657 vector(WT-LINC00657)and mutant LINC00657 vector(MUT-LINC00657).Results Compared with controls,the expression of LINC00657 was upregulated and the expression of miR-224-3p was downregulated in OGD-induced HT22 cells(P＜0.05).Compared with trans-fection of si-NC or miR-NC,transfection of si-LINC00657 or miR-224-3p mimics resulted in in-creased cell viability,SOD activity,and GSH-Px activity,as well as decreased apoptosis rate,LDH activity,and MDA level(P＜0.05).Overexpression of miR-224-3p reduced the luciferase activity of WT-LINC00657(P＜0.05).Compared with cells co-transfected with si-LINC00657 and anti-miR-NC,cells co-transfected with si-LINC00657 and anti-miR-224-3p showed decreased cell viabili-ty,increased apoptosis rate,increased LDH activity and MDA level,and decreased SOD and GSH-Px activities(P＜0.05).Conclusion Interference with LINC00657 can promote cell proliferation,inhibit apoptosis and oxidative stress response by upregulating miR-224-3p,thereby alleviating OGD-induced injury in mouse hippocampal neurons.

Objective To investigate the effects and mechanisms of LINC00657 on oxidative glu-cose deprivation(OGD)-induced injury in mouse hippocampal neurons.Methods Mouse hippocam-pal neuron cell line HT22 was given OGD treatment to establish an injury model,with normally cul-tured HT22 cells as controls.The si-NC,si-LINC00657,microRNA(miR)-NC,and miR-224-3p mimics were transfected into HT22 cells,followed by OGD treatment.Co-transfection of si-LINC00657 and anti-miR-NC,or co-transfection of si-LINC00657 and anti-miR-224-3p,was performed in HT22 cells before OGD treatment.Real-time quantitative reverse transcription-polymerase chain reaction(qRT-PCR)was used to detect the relative expression levels of LINC00657 and miR-224-3p.CCK-8 assay and flow cytometry were used to detect cell viability and apoptosis rate,respectively.Kits were used to detect the activities of lactate dehydrogenase(LDH),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and the level of malondialdehyde(MDA).Dual-luciferase reporter gene assay was used to detect the effect of miR-224-3p overexpression on the luciferase activity of wild-type LINC00657 vector(WT-LINC00657)and mutant LINC00657 vector(MUT-LINC00657).Results Compared with controls,the expression of LINC00657 was upregulated and the expression of miR-224-3p was downregulated in OGD-induced HT22 cells(P＜0.05).Compared with trans-fection of si-NC or miR-NC,transfection of si-LINC00657 or miR-224-3p mimics resulted in in-creased cell viability,SOD activity,and GSH-Px activity,as well as decreased apoptosis rate,LDH activity,and MDA level(P＜0.05).Overexpression of miR-224-3p reduced the luciferase activity of WT-LINC00657(P＜0.05).Compared with cells co-transfected with si-LINC00657 and anti-miR-NC,cells co-transfected with si-LINC00657 and anti-miR-224-3p showed decreased cell viabili-ty,increased apoptosis rate,increased LDH activity and MDA level,and decreased SOD and GSH-Px activities(P＜0.05).Conclusion Interference with LINC00657 can promote cell proliferation,inhibit apoptosis and oxidative stress response by upregulating miR-224-3p,thereby alleviating OGD-induced injury in mouse hippocampal neurons.

Apoptosis ; Calcineurin ; metabolism ; Chymotrypsin ; metabolism ; Humans ; Lysosomes ; enzymology ; Mitochondria ; metabolism ; pathology ; Mitochondrial Dynamics ; Neuroblastoma ; metabolism ; pathology

Objective: To analyze the effects of twirling reinforcing and reducing manipulations on protein expres-sion in parietal cortex of spontaneously hypertensive rats (SHRs), and elucidate the main mechanisms and differences between two manipulations in hypertension treatment. Methods: Rats were randomly divided into the control, model, twirling reinforcing manipulation (TRFM), and twirling reducing manipulation (TRDM) groups. The control and model groups received catch and fixation stimulations once a day for 14 days. The TRFM and TRDM groups were intervened once a day for 20 min for 14 days. On days 0, 2, 6, 10, and 14 after acupuncture, rat systolic blood pressures (SBPs) were measured. Differential protein (DP) expression in the rat parietal cortices was detected. Thereafter, GO functional significance and KEGG pathway enrichment analyses were performed. Results: Compared with the model group, SBP of rats in the TRDM and TRFM groups decreased on days 6 and 10 of acupuncture, respectively (P=.009; P<.001). Moreover, SBP of the TRDM group was signif-icantly lower than that of the TRFM group on days 10 and 14 of acupuncture (P = .015; P = .013). Compared with control group, 601 and 1040 DPs were up-and downregulated, respectively, in the model group. Compared with model group, 44 and 28 up-and downregulated DPs were expressed, respectively, in the TRFM group. Compared with model group, expression of 616 and 427 up-and downregulated DPs, respectively, was found in the TRDM group. After combining the results of GO and KEGG enrichment analyses, five and one pathways were found to be related to the central antihypertensive mechanism of the parietal cortex during twirling reducing and reinforcing manipulations, respectively. Conclusion: TRDM showed a more effective antihypertensive effect on SHRs than TRFM; this antihy-pertensive effect was related to the regulation of different proteins and their biological functions.

Objective:To investigate the data on death cause of residents around Sanmen nuclear power plant from 2015 to 2019.Methods:The data on death cause of residents in Sanmen county from 2015 to 2019 were collected, and the top 10 diseases in death causes ranking and radiation-related malignant tumors were analyzed.Results:The average mortality rate of residents in Sanmen county was 575.87 per 100 000 population from 2015 to 2019, lower than the national level and close to that in Zhejiang Province. The top 10 death causes remain unchanged, higher than 95.79% of total deaths. The mortality rate of malignant tumor increased from 159.23 per 100 000 population in 2015 to 191.51 per 100 000 population in 2019 (χ 2=15.889, P<0.05). There was no significant difference in the proportion of mortality from radiation-related tumor(leukemia and thyroid cancer) in Sanmen county in recent 5 years ( P>0.05). Conclusions:From 2015 to 2019, the death rates of residents in Sanmen county were relatively stable. The effects of the operation of the nuclear power plant on the health of the local residents need to be continuously monitored.

【Objective】 To analyze the independent influencing factors of repeated extracorporeal shock wave lithotripsy (ESWL) in the treatment of upper urinary calculi (UUC), based on which a nomogram model was established to predict the efficacy. 【Methods】 Clinical and imaging data of 203 patients receiving repeated ESWL during Jan.2020 and Dec.2022 were collected, including 117 cases in the successful group and 86 cases in the unsuccessful group.The patients’ age and sex, stone volume (SV), surface area (SA), skin-to-site distance (SSD), maximum CT value, mean stone density (MSD), and stone heterogeneity index (SHI) were compared between the two groups.The independent predictors were analyzed with logistic regression and the meaningful variables (P<0.05) were used to establish a nomogram.The efficacy of the model was evaluated using receiver operating characteristic (ROC) curve and decreasing curve analysis (DCA).Internal validation was also performed. 【Results】 Stepwise regression showed that SV, SSD, MSD and SHI were independent influencing factors (P<0.05).The area under the ROC curve (AUC), optimal threshold, sensitivity and specificity were 0.793 (95%CI: 0.674-0.911), 0.619, 77.1% and 74.0%, respectively.The DCA curve was above two extreme curves.Hosmer-Lemeshow test and calibration curve showed that the nomogram had a good fitting degree (χ²=5.526, P=0.489), and the correction C-index was 0.746. 【Conclusion】 SV, SSD, MSD and SHI are independent predictors of the efficacy of repeated ESWL in the treatment of UUC.The nomogram established based on the above indicators has good predictive efficiency and clinical applicability.

Objective:To investigate the association between hyperuricemia and the risk for stroke occurrence, as well as the mediating effect of hypertension on this association.Methods:In this study, the China Chronic Diseases and Nutrition Surveillance system in 2015 was used as baseline data. We identified hospital admissions for stroke using the electronic homepage of inpatient medical records from 2013-2020, and death data were obtained from the 2015-2020 National Mortality Surveillance System. A retrospective cohort was established after matching and linking the database. The Cox proportional hazard regression model was used to analyze the relationship between hyperuricemia and the risk of stroke and its subtypes. Restricted cubic spline analysis was conducted to examine the dose-response relationship between serum uric acid levels and the risk for stroke. Mediation analysis was performed to investigate the mediating effect of hypertension on the association between hyperuricemia and the risk for stroke and its subtypes. Subgroup analyses were conducted based on gender and age groups.Results:A total of 124 352 study subjects were included, with an accumulative follow-up time of 612 911.36 person-years. During the follow-up period, 4 638 cases of stroke were found, including 3 919 cases of ischemic stroke and 689 cases of hemorrhagic stroke. The incidence density of stroke was 756.72 per 100 000 person-years, 641.37 per 100 000 person-years for ischemic stroke, and 114.60 per 100 000 person-years for hemorrhagic stroke. Multivariable Cox proportional hazards regression models showed that after adjusting for covariates, compared to those without hyperuricemia, individuals with hyperuricemia had a 16% higher risk for stroke [hazard ratio ( HR)=1.16, 95% CI: 1.06-1.27], a 12% higher risk of ischemic stroke ( HR=1.12, 95% CI: 1.01-1.24), and a 39% higher risk of hemorrhagic stroke ( HR=1.39, 95% CI: 1.11-1.75). Mediation analysis showed that hypertension partially mediated the associations between hyperuricemia and the risk for stroke, ischemic stroke, and hemorrhagic stroke, with mediation proportions of 36.07%, 39.98%, and 25.34%, respectively. The mediating effect is pronounced in the male population and individuals below 65. Conclusion:Hyperuricemia is a risk factor for stroke, and hypertension partially mediates the effect of hyperuricemia on stroke.

【Objective】 To compare the quantitative detection results of two domestic quantitative real-time PCR reagents in HBV-DNA detection. 【Methods】 A total of 306 serum samples form hepatitis B patients were selected for quantitative parallel detection of high-sensitiveity HBV-DNA using domestic reagent A and B, and the difference and consistency of the results were analyzed. 【Results】 1) The yielding rate of reagent A and B was 86.92% and 84.64%, respectively. The regression linear equation was Y=0.984 9x+ 0.154 9, R2=0.945 7, the correlation coefficient r=0.972 5, indicating the results by the two reagents had good correlation. 2) When the concentration was in the range of(20~1 000) IU/mL, the yielding rates of reagent A and B were 39.9% and 37.6%, respectively. 【Conclusion】 Reagent A is more suitable for post-treatment monitoring in patients with OBI and HBeAg(-), but also can be used to detect HBV pathogens in patients before operations or blood transfusions.