Objective To explore the influence of continuous veno-venous hemofiltration (CVVH) therapy on cardiac index (CD, global end-diastolic volume index (GEDI) and extravascular lung water index (ELWI) monitored by Pulse-indicated continuous cardiac output (PiCCO). Methods The 12 critically ill patients with acute renal failure were selected consecutively from department of intensive care unit (ICU) in Beijing Hospital. The patients who received CVVH therapy were monitored by PiCCO plus device. The hemodynamic measurements were performed during the process and interruption of CVVH. Pared t-test was used to analyze the values of CI, GEDI and ELWI. Results A total of 48 groups of data from 12 patients were analyzed. There were no significant differences in CI [(4.75±0.93) L ? min(-1) · m(-2)vs. (4.69±0.89) L · min(-1) · m(-2)], GEDI [(780.60±109.30) ml/m2 vs. (784.75± 106.20) ml/m2] and ELWI CC11.61±3.45) ml/kg vs. (11.54±3.56) ml/kg] between the interruption and process of CVVH, as obtained by the pared t-test (all P>0.05). Conclusions The CVVH therapy has no effect on the accuracy and reliability of PiCCO measurement of CI, GEDI and ELWI.

With redox-sensitive fluorescene probes DCFH-DA and DHR123, the formation of cytosolic and intramitochondrial reactive oxygen species (ROS) inside immature rat cerebellar granule cells during the apoptosis induced by nitric oxide donor S-nitroso-N-acetyl-pennicillamine (SNAP) was monitored by laser confocal scanning microscopy. The cytosolic and intramitochondrial ROS increase significantly after 0.5 mmol/L SNAP treatment for 1 h. Pre-treatment with the nitric oxide scavenger hemoglobin can effectively inhibit the formation of cytosolic and intrarnitochondrial ROS and protect neurons from apoptosis. Adding glutathione can also protect neurons from apoptosis, and the cytotoxity of nitric oxide increases significantly while the synthesis of glutathione is inhibited. The results indicated that ROS might be involved in NO-induced apoptosis in neural cells and glutathione might be the endogenesis antioxidant to protect neurons from oxidative injury.

Objective To investigate effects of Levosimendan on hemodynamics in heart failure patients with low cardiac output. Methods A total of 27 heart failure patients with low cardiac output were recruited in the surgical intensive care unit (SICU)of Beijing Hospital from July 2009 to May 2016. All patients were treated with continuous intravenous infusion of Levosimendan 12.5 mg within 24 h at a rate of 0.1~0.2 μg· kg -1·min-1. Hemodynamic variables were monitored by pulse-indicated continuous cardiac output(PICCO)at baseline ,24 h ,4 d and 7 d after infusion. Results There were no significant differences in systolic blood pressure(SBP) ,diastolic blood pressure(DBP) ,heart rate(HR)and global end diastolic volume index (GEDI)as comparing baseline with 24 h ,4 d and 7 d after infusion of levosimendan. The significant differences were found in central venous pressure (CVP) ,systemic vessel resistance index (SVRI) , extravascular lung water index (EVLWI ) among baseline versus 24 h ,4 d and 7 d after infusion of levosimendan[(8.34± 2.87)mmHg vs.(6.35± 2.31),(6.81± 2.03),(5.92± 2.23)mmHgforCVP ,(2 682.0 ± 388.9)dyn·s-1·cm -5·m-2vs. (2 170.0 ± 410.9) ,(2 062.0 ± 340.6 ) ,(1 960.0 ± 380.2 )dyn ·s-1·cm-5·m-2for SVRI ,(8.90 ± 2.45)ml/kg vs. (6.60 ± 2.66) ,(5.92 ± 2.88) ,(5.46 ± 2.52) ml/kg for EVLWI ,P=0.001 ,0.003 or＜0.01 ,respectively].As compared with hemodynamic variables at baseline ,brain natriuretic peptide levels were decreased ,and cardiac index ,cardiac function index and stroke volume index were increased at 24 h ,4 d and 7 d after infusion of Levosimendan (P＜0.01 ,0.05 or 0.01 , respectively ). Conclusions A single-dose intravenous infusion of Levosimendan can increase cardiac output and decrease systemic vascular resistance in heart failure patients with low cardiac output ,which maintain quite a long time.

Objective To investigate the clinical value of serum interleukin-6(IL-6)and inter-leukin-8(IL-8)levels in combination with pulmonary function indicators in identifying severe Myco-plasma pneumoniae pneumonia(MPP)in children.Methods A total of 102 children with severe MPP were selected as the study subjects(severe group),and another 102 children with mild MPP treated during the same period were selected as the mild group.The serum levels of IL-6,IL-8 as well as forced vital capacity(FVC),forced expiratory volume in one second(FEV1),FEV1 percent-age of predicted(FEV1％pred)and the ratio of FEV1 to FVC at the time of admission of children in each group were compared.Results The serum levels of IL-6 and IL-8 in the severe group were sig-nificantly higher than those in the mild group,while the FVC,FEV,％pred,and FEV1/FVC％were significantly lower than those in the mild group(P＜0.05).The serum levels of IL-6 and IL-8 were negatively correlated with FVC,FEV1％pred and FEV1/FVC％(P＜0.05).The area under the curve(AUC)for assessing the severity of MPP for IL-6,IL-8,FVC,FEV1％pred and FEV1/FVC％was 0.894(0.849 to 0.939),0.842(0.787 to 0.897),0.896(0.851 to 0.942),0.787(0.725 to 0.849)and 0.744(0.676 to 0.812),respectively.The AUC for the combined assessment of the above five parameters was 0.985(0.973 to 0.997).Conclusion Serum IL-6,IL-8 and pulmonary function indicators can reflect the severity of MPP in children.Their combined detection can be used to differentiate between mild and severe MPP in children early and timely.

Objective To investigate the clinical value of serum interleukin-6(IL-6)and inter-leukin-8(IL-8)levels in combination with pulmonary function indicators in identifying severe Myco-plasma pneumoniae pneumonia(MPP)in children.Methods A total of 102 children with severe MPP were selected as the study subjects(severe group),and another 102 children with mild MPP treated during the same period were selected as the mild group.The serum levels of IL-6,IL-8 as well as forced vital capacity(FVC),forced expiratory volume in one second(FEV1),FEV1 percent-age of predicted(FEV1％pred)and the ratio of FEV1 to FVC at the time of admission of children in each group were compared.Results The serum levels of IL-6 and IL-8 in the severe group were sig-nificantly higher than those in the mild group,while the FVC,FEV,％pred,and FEV1/FVC％were significantly lower than those in the mild group(P＜0.05).The serum levels of IL-6 and IL-8 were negatively correlated with FVC,FEV1％pred and FEV1/FVC％(P＜0.05).The area under the curve(AUC)for assessing the severity of MPP for IL-6,IL-8,FVC,FEV1％pred and FEV1/FVC％was 0.894(0.849 to 0.939),0.842(0.787 to 0.897),0.896(0.851 to 0.942),0.787(0.725 to 0.849)and 0.744(0.676 to 0.812),respectively.The AUC for the combined assessment of the above five parameters was 0.985(0.973 to 0.997).Conclusion Serum IL-6,IL-8 and pulmonary function indicators can reflect the severity of MPP in children.Their combined detection can be used to differentiate between mild and severe MPP in children early and timely.

Lipid Droplets/metabolism* ; Bacteria ; Lipid Metabolism