Objective To investigate the risk factors for intracranial atherosclerosis in patients with ischemic stroke.Methods The demographic data and vascular risk factors in patients with ischemic stroke who performed digital subtraction angiography were analyzed retrospectively.According to the lesion locations,the patients with moderate to severe intracranial arterial stenosis were divided into intracranial internal carotid artery,middle cerebral artery,intracranial vertebral artery,and basilar artery.The démographic data and vascular risk factors among all groups were compared.Results A total of 344 patients with ischemic stroke were recruited,147 (42.7％ ) of them were moderate to severe intracranial arterial stenosis and 197 (57.3％ ) were mild intracranial arterial stenosis.Univariate analysis showed that the proportion of diabetes (34.0％ vs.20.8％,x2 =7.541,P=0.006) or hyperlipidemia (78.9％ vs.66.0％,x2 =6.900,P=0.009) in the moderate to severe intracranial arterial stenosis groups was significantly higher than that in the mild intracranial arterial stenosis group.The levels of total cholesterol (4.656 ±0.955 mmol/L vs.4.401 ±0.882 mmol/L,t =-2.543,P =0.011),low-density lipoprotein cholesterol (3.015 ± 0.817 mmol/L vs.2.741 ± 0.786 mmol/L,t =-3.113,P =0.002),fasting blood glucose (FBG) (6.184 ± 2.127 mmol/L vs.5.568 ±1.772 mmol/L,t =-2.869,P =0.004),and the National Institutes of Health Stroke Scale score (5.48 ± 4.980 vs.4.33 ± 4.094,t =-2.332,P =0.020) were significantly higher.Multivariable logistic regression analysis showed that diabetes (odds ratio [ OR ] 1.907,95％confidence interval [ CI] 1.164-3.124; P =0.010) and low-density lipoprotein cholesterol (OR 1.500,95％ CI 1.133-1.986; P =0.005) were the independent risk factors for moderate to severe intracranial arterial stenosis.Among the patients with different locations of intracranial arteries,there were significant differences in the distribution of the risk factors,such as male (P =0.017),coronary artery disease (P =0.002),and smoking (P =0.026).Conclusion Diabetes and the increased level of low-density lipoprotein cholesterol were the independent risk factors for moderate to severe stenosis of intracranial arteries.The distribution of risk factors for intracranial atherosderosis had location specificity.

Objective:To examine the influence of exam stress on the sustained attention of students with different levels of trait anxiety. Methods: With the State-Trait Anxiety Inventory (STAI), 10 students with high trait anxiety (high trait anxiety group, H group) and 10 with non-high trait anxiety (non-high trait anxiety group, NH group) were randomly sampled from 400 students taking the coming College English Test-Band 4 (CET-4). Their attention was measured with digit cancellation test and the event related potentials during the control phase (the period was chosen 2 weeks after the beginning of the semester and there was no examination in the following 3 months) and the stress phase ( from 2 weeks to 1 day before CET-4 examination). Results: The right cancellation number of the NH group was higher than that of the H group(F=8.178, P=0.007). During the control phase the P300 amplitude and latency were similar between the 2 groups (F= 0.125, 0.127, respectively;P= 0.728, 0.726, respectively). During stress phase the P300 amplitude of the H group was significantly lower than that of the NH group(F=12.913, P=0.002)and the P300 latency of the H groups was longer than that of the NH group(F=16.952, P= 0.001). In NH group, the P300 latency in the stress phase was significantly shorter than that of the control phase (F= 6.514, P=0.020). Conclusion: Exam stress has different influences on the sustained attention of people with different levels of trait anxiety, which suggests that people with high trait anxiety are the target population for stress management.

Objective To investigate risk factors and prognosis of hemorrhagic transformation(HT)in acute cerebral infarction patients treated by intravenous thrombolysis with recombinant tissue plasminogen activator(rt-PA).Methods All 128 patients with acute cerebral infarction were treated with intravenous rtPA within 6 hours from stroke onset.The clinic records and laboratory datas of pre-and post-treatment were statistically analyzed between HT group and non-HT group to find potential risk factors to HT and contributors of prognosis.Results HT occurred in 29 patients(22.66％),including 16 patients with symptomatic ICH(12.50％)and 2 patients died(6.90％ of HT).Logistic regression analysis showed that history of atrial fibrillation(OR =1.293,95％ CI 1.224-1.589,P =0.001),CT density changes with mass effect or edema(OR =2.452,95％ CI 1.132-3.309,P =0.034),diastolic blood pressure ≥ 100 mm Hg before thrombolytic therapy(1 mm Hg =0.133 kPa,OR =9.265,95％ CI 1.435-59.836,P =0.019),blood glucose ≥ 11.1 mmol/L(OR =3.037,95％ CI 0.252-57.593,P =0.047),NIHSS score ＞ 15 points (OR =8.752,95％ CI 1.035-30.285,P =0.023)and thrombolysis time ＞ 3 h(OR =98.74,95％ CI 5.067-186.120,P =0.002)are independent risk factors for HT; among these factors,baseline blood glucose(OR =3.265,95 ％ CI 0.435-59.863,P =0.045),NIHSS score(OR =10.453,95 ％ CI 5.647-38.185,P =0.003)and thrombolysis time(OR =2.541,95％ CI 1.098-51.086,P =0.017)also are prediction factors of the prognosis of HT.Conclusion Risk factors associated with HT are diastolic blood pressure before thrombolysis,glucose level,degree of neurological deficits,CT early changes,atrial fibrillation and thrombolytic time.Glucose level,neurological deficits and thrombolysis time affects the prognosis of patients.

Objective To study the effect and mechanism of senegenin on proliferation and differentiation of neural stem cells (NSCs). Methods The primary cultured NSCs were divided into the high-dose, medium-dose, low-dose group and normal control group (NC). The complete medium containing 10, 20 and 40 μmol/L senegenin was added to senegenin low-, middle-, and high- dose groups, and the NC group was routinely cultured. After 4 days of culture, CCK8 assay was used to detect cell viability, and microscopy was performed and the number of neurospheres was counted. Western blot was used to detect the expression of Nestin, TUJ1, GSK-3β, and p-GSK-3β (Ser9), and immunofluorescence staining was used to visualized Nestin and TUJ1. Results Compared with the control group, the number of NSCs neurospheres (32.78 ± 6.30, 40.93 ± 8.34, 45.37 ± 7.96 vs. 26.48 ± 5.19) and the proliferation (127.50% ± 9.31%, 138.13% ± 6.88%, 151.25% ± 9.38% vs. 100.00% ± 5.63%) in the low-, middle- and high-doses of senegenin group significantly increased (P<0.05 or P<0.01).The expression of TUJ1(2.21 ± 0.14,3.10 ± 0.16,3.30 ± 0.15 vs.1.00 ± 0.00)in the low-,middle- and high-doses of senegenin group significantly increased (P<0.05); and the expression of Nestin (0.36 ± 0.04,0.53 ± 0.05,0.46 ± 0.05 vs.1.00 ± 0.00)significantly decreased(P<0.05).The ration of p-GSK-3β(Ser9)/GSK-3β(2.31 ± 0.17,3.41 ± 0.11,3.59 ± 0.16 vs.1.00 ± 0.00)in the low-,middle-and high-doses of senegenin group significantly increased(P<0.01).The cell number of Nestin+(50.29 ± 3.18,45.28 ± 6.23,38.72 ± 5.31 vs. 75.27 ± 6.03) in the low-, middle- and high-doses of senegenin group significantly decreased (P<0.05 or P<0.01), and the cell number of TUJ1+(32.23 ± 4.36,38.23 ± 6.01,46.23 ± 4.36 vs.20.31 ± 5.23)significantly increased (P<0.01). Conclusions The senegenin may promote the proliferation and differentiation of NSCs through the activation of Wnt pathway.

According to the course teaching requirements of psychiatry,a serial teaching cases of case-based learning (CBL) is built based on cases from psychology department to assist in the theory teaching of Psychiatry.During the teaching process,the scenes where patients came to the psychology department were simulated and the information about the cases was provided step by step.For each step,students would be asked to discuss a series of questions regarding the cases in different groups.Along with CBL,students could thoroughly learn the main points of diagnosis and principles of treatment for common psychiatric disorders.The teaching effect showed that students' involvement in CBL courses,the interests in learning,self-study ability,independent thinking ability,capacity for self-expression,ability of creative thinking and comprehensive analysis and problem solving ability.were greatly improved.Most students were interested in CBL and it produced fruitful teaching results.

Non-suicidal self-injury(NSSI) is defined as deliberate " destruction to body issue without conscious suicidal intent" and which is not culturally and socially sanctioned.The evaluation of NSSI mainly includes explicit measurement and implicit measurement.In recent years, implicit measurement has received wide attention from scholars, among which the implicit association task(IAT), as a tool to measure the relative attitude towards objects in individuals' implicit cognition and to prevent the interference of consciousness, which is an effective method to evaluate NSSI.On the basis of introducing the background of implicit attitude, this paper summarized the research progress of implicit attitude in distinguishing and predicting NSSI from behavioral and neuroimaging perspectives.Firstly, compared with the group without NSSI history, the NSSI group had a more recognized implicit attitude towards NSSI, which was correlated with the frequency and severity of NSSI.Secondly, compared with other known traditional predictors, whether implicit attitude is a better predictor of subsequent behavior of NSSI remains controversial.Finally, there are not many studies on the biological basis of implicit attitude towards NSSI in NSSI populations.Preliminary results suggest that the activation of salience networks and the reduction of gray matter volume in some brain regions (such as the dorsal striatum) may be related to implicit attitude in NSSI populations.To sum up, measuring implicit attitudes towards NSSI is useful for screening and predicting people at risk for NSSI, especially when individuals have false reports or low self-awareness.In addition, this paper also put out some shortcomings for future research and clinical intervention.

Objective Studies have shown that problem based learning (PBL) can improve the students' ability of autonomous learning.However,it is unclear whether students' autonomous learning ability will affect students' evaluation in PBL teaching.This study took medical psychology course as an example to explore the influence of medical students' autonomous learning ability on PBL teaching effect.Methods 43 8-year program medical students of Grade 2010 and 2011 were enrolled in this study.Scale was used to assess medical students' autonomous learning ability before the start of the PBL teaching.Self made questionnaire was used to survey the students' evaluation in PBL teaching.Linear correlation analysis was used to detect the relationship between medical students' autonomous learning ability and students' evaluation in PBL teaching and their examination results.Results Medical students' autonomous leaming ability is significantly positively correlated to the students' evaluation of PBL teaching (P＜0.05).The correlation coefficient between learning motivation and "PBL enhanced learning interest" or "PBL enhanced information utilization ability" was high (r=0.507 and 0.536,respectively).It showed that students with stronger autonomous learning ability had more positive evaluation of PBL teaching.The students' learning motivation was also positively correlated to the test score (r=0.416,P=0.006).Students with stronger learning motivation had higher test score in the final exam.Conclusion These results indicate that the relationship between the students' autonomous learning ability and PBL teaching is bidirectional.The students with more powerful ability of autonomous learning will be more active in the process of PBL teaching,and the positive experience will promote their autonomous learning ability in turn.