Objective To investigate the association between matrix metalloproteinase 9(MMP 9) and type 2 diabetic macroangiopathy and explain the mechanism of Simvastatin protective effects on blood vessel.Methods The serum MMP 9 was determined by ELISA in 40 healthy controls and 80 type 2 diabetes(including 40 type 2 diabetic macroangiopathy and 40 type 2 diabetes without complications) before and after Simvastatin treatment.Results The serum MMP 9 in type 2 diabetic macroangiopathy was higher than that in type 2 diabetes without complications and healthy controls.Serum MMP 9 was correlated positively with TC,LDL C,Ox LDL and blood pressure and correlated negatively with HDL C. After Simvastatin treatment,the levels of serum MMP 9 were reduced and serum TC,LDL C and Ox LDL were also decreased.Conclusion MMP 9 may play an important role in the development and progression of type 2 diabetic macroangiopathy.The protective effects of Simvastatin on blood vessel partly attribute to its lowering of serum MMP 9.

Objective To study the significance of phosphatidyl inositol 3-kinase (PI3K) expression in amyotrophic lateral sclerosis (ALS) atrophy muscle fibers and investigate the pathogenesis of muscle fiber atrophy.Methods The clinical data of 90 ALS inpatients,admitted to our hospital from June 2005 to January 2013,were collected; and the features of clinical manifestations,creatine kinase level and electrophysiology were investigated.All patients accepted open muscle biopsy; the frozen tissues were performed histochemical,enzymology,anti-PI3K and nerve cell adhesion molecules (NCAM) monoclonal antibody immunohistochemistry staining; the pathological and ultrastructure characteristics were observed.Results A lot of small angular fibers,atrophy fibers and nuclear clump grouping were observed in skeletal muscle samples of ALS.PI3K positively expressed in sarcolemma of atrophy fibers,while NCAM negatively expressed.The pathologic changes of ultrastructure in transmission electromicroscope illustrated ALS atrophy fibers,having typical mitochondria paramorphia changes.Conclusion Mitochondria paramorphia changes and dysfunction are observed in ALS muscle fibers; atrophy fibers of ALS are related to muscle fiber apoptosis.

Objective To study the clinical,pathological and molecular biology features of myotonic myopathies.Methods Eighty-one patients with myotonic myopathies,admitted to our hospital from June 2005 to June 2018,were chosen in our study.All patients accepted clinical and skeletal muscle pathology examination,and genetic features of 55 patients were analyzed by molecular biological method.Results (1) All patients suffered from typical myotonia,and electromyography shows typical myotonic discharges;47 patients exhibited myotonic dystrophy (DM) and 34 patients exhibited non-myotonic dystrophy (NDM).(2) In muscle biopsy of DM,typical central nuclei,pyknotic clumps and sarcoplasmic masses were observed;and characteristic pathological changes were not observed in muscle biopsy of NDM.(3) Totally,32 DM1 patients,3 DM2 patients,9 MC patients and 5 paramyotonia congenita patients were confirmed by molecular biology technology;7 independent mutations in the CLCN1 gene and 3 independent mutations in the SCN4A gene were novel mutations.Conclusions (1) Myotonic myopathies are some single gene inheritance diseases with multisystem disorders and their main symptoms include myotonia.(2) Skeletal muscle biopsy is a trustworthy method for definite diagnosis of myotonic myopathies;gene analysis is the gold standard for diagnosis and classification ofmyotonic myopathies.

Objective To observe the temporary loss(re-falling)of knee range of motion(ROM)during rehabilitation after arthroly-sis for post-traumatic knee stiffness,and analyze the factors related to it. Methods From July,2016 to September,2019,64 patients(68 knees)in Beijing Bo'ai Hospital accepted minimally inva-sive arthrolysis for post-traumatic knee stiffness,and were followed up for twelve months.ROM of flexion and extension of knee was measured before operation,and one,six and twelve weeks,and six and twelve months af-ter operation;while they were asssessed with Hospital for Special Surgery Knee Score(HSS).Multivariate Logis-tic regression was performed on re-falling. Results The ROM and HSS score improved as a whole after operation;however,HSS score improved constantly,but ROM decreased six weeks after operation compared with that one weeks after operation,involving 46 knees of 44 cases.Arthrolysis longer than 12 months from primary injuries,multiple complicated fracture and history of infection were the risk factors for re-falling(OR＞8.058,P＜0.05). Conclusion Minimally invasive arthrolysis is effective on knee function after arthrolysis for post-traumatic knee stiff-ness.However,re-falling of ROM may happen during rehabilitation.Delay of operation,multiple complicated fracture and history of infection may increase the risk of re-falling.