ObjectiveTo investigate the clinical value ofDNA ploidy analysis in the diagnosis of benign and malignant pleural effusion.MethodsDNA ploidy in 24 benign pleural effusion and 39 malignant pleural effusion were detected by flow cytometry (FCM) and compared with the results of cytologic detection at the same time.ResultsThe positive rates of FCM detection in benign and malignant pleural effusion were 8.33％(2/24) and 64.10％ (25/39),there was significant difference (P＜0.05).The positive rates of cytologic detection in benign and malignant pleural effusion were 4.17％( 1/24 ) and 53.85％( 21/39),there was significant difference (P＜0.05).The sensitivity of FCM and cytologic detection in malignant pleural effusion was 64.10％ (25/39) and 53.85％ (21/39),the specificity of two methods was 91.67％ (22/24) and 95.83％ (23/24.),the results of two methods showed no significant differences (P ＞0.05).ConclusionDNA ploidy analysis by FCM has important clinical value in the diagnosis of benign and malignant pleural effusion.

Objective To study the related factors of coronary no-reflow phenomenon in H-hypertensive acute myocardial infarction (AMI) patients after percutaneous coronary intervention (PCI).Methods 120 AMI patients were selected as the study subjects,all patients were given PCI,the infarct-related coronary artery stenosis forward flow(TIMI) grade ≦ 2 level was the no-flow criteria.The patients were divided into two groups:the no-reflow group (35 patients),the other 85 cases classified as normal blood group.The age,gender,body mass index (BMI),history of diabetes,hyperlipidemia hyperlipidemia history,family history,blood glucose,homocysteine (Hcy),white blood cell count,C-reactive protein (CRP),erythrocyte sedimentation rate (ESR),creatine kinase (CK),creatine kinase (CK-MB),triglycerides,total cholesterol,high density lipoprotein (HDL),low density lipoprotein (LDL),troponin-Ⅰ (cTn-Ⅰ),treatment time and other factors were observed and compared.Logistic regression analysis was used to identify relevant factors of no-reflow.Results 120 patients with primary PCI,no-reflow in 35 patients,the incidence rate was 30.1％.The sex,smoking history,history of hyperlipidemia,diabetes,history of angina,and Killip classification before treatment,time from chest pain to before treatment between no-reflow patients and normal blood flow patients had no significant differences (t =1.275,P ＞ 0.05).Multivariate Logistic regression analysis showed that:age,diabetes,triglycerides,HCY,cTn-Ⅰ,CK-MB,CRP were related factors of no-reflow(t =3.427,P ＜ 0.05).Conclusion Age,without pre-infarction history of angina,coronary artery open long hours,admission NYHA low,white blood cell count high,CK-MB and cTn-Ⅰ levels higher are independent risk factors of no-reflow phenomenon,PCI with no-reflow phenomenon indicates the poor prognosis.

Objective: To evaluate the diagnostic value of IL-6,IL-8,and TNF-? in malignant pleural effusion and tuberculous pleural effusion.Methods:We detected IL-6,IL-8,and TNF-? in malignant pleural effusion(21 cases)and tuberculous pleural effusion(13cases).Results:The mean values of the three cytokines measured in tuberculous pleural effusion were significantly higher than malignant pleural effusion( P

Objective:To implove the level of diagnosis and treatment of renal tuberculosis complicated with active pulmonary tuberculosis.MethodsThe diagnosis and treatment of 28 cases with renal tuberculosis complicated with active pulmonary tuberculosis were analyzed.Results:88.2% of patients were negative in PPD test, and 55.6% positive in the sputum acid fast stain. All the patients except 4 received thetreatment of anti-tuberculosis continuosly for 3-6 months before surgery.Conclusion:It is necessary to treat the patients with anti-tuberculosis drugs and improve their immune function before proper surgical treatment.Patients in fair general condition could be operated on 2 weeks after anti-tuberculosis therapy.

A method was developed for the determination of 39 polybrominated diphenyl ethers(PBDEs) from mono- to hepta-brominated in coastal sediment by gas chromatography/mass spectrometry. The sample was extracted with a solution of dichloromethane: n-hexane( 1:1,V/V) ,followed by ultrasonic extraction for 60 min at 25℃. The clean-up step was optimized using silica gel and alumina. The detection limit of the method was in the range of 0.003 - 0.10μg/kg. The results indicate that the average recovery of the internal standard was from 66.2% to 118. 6%. The relative standard deviation RSD( % ) was from 0. 8 % to 18. 2%. The method for the detection of 39 PBDE congeners in coastal sediment was sensitive,high reproducible with satisfactory recoveries.

Objective To investigate the effects of preoperative biliary drainage ( PBD ) on the morbidity and mortality of pancreaticoduodenectomy in patients with malignant obstructive jaundice in the lower bile duct. Methods Clinical data of 74 cases undergoing pancreaticoduodenectomy from Jan. 2000 to Dec. 2005 with preoperative total bilirubin level over 85 μmol/L were collected and retrospectively analyzed. Comparison was performed between patients receiving PBD and those not. Before surgery. The parameters sincluding perioperative situation, in-hospital death rate, post-operative complications and were calculated to evaluate the influence of pre-operative biliary drainage on the outcomes. Univariate analysis and Logistic analysis were used to identify the risk factors for post-operative complications. Results Forty (40/74) cases received PTCD procedure. The total bilirubin was significantly reduced from (338. 10±88. 38 )μwnol/L to ( 228. 50±82.24) μ,mol/L in PTCD population and was significantly lower than ( 328. 60±93. 02) μmol/L of the non-PTCD group. There was no significance between the two groups in terms of total complications and individual complication Logistic regression analysis showed that preoperative TB over 340 μmol/L and blood loss over 600 ml were important risk factors for post-operative complications. Conclusion High pre-operative total bilirubin over 340 μmol/L increases the risk of post-operative complications in the patients with malignant jaundice. Preoperative biliary drainage is useful to reduce the total bilirubin and improve the hepatic function of the patients. Prolonged pre-operative biliary drainage could be considered in the patients with high preoperative bilirubin,poor nutritional condition and impaired coagulation to enhance the tolerance of surgery.

The study of brain information flow is of great significance to understand brain function in the field of neuroscience. The Granger causality is widely used functional connectivity analysis using multivariate autoregressive model based on the predicted mechanism. High resolution electroencephalogram (EEG) signals of ten healthy subjects were collected with a visual selective attention task. Firstly, independent component analysis was used to extract three spatially independent components of the occipital, parietal, and frontal cortices. Secondly, the Granger causal connectivity was computed between these three regions based on the Granger causality method and then independent sample t-test and bootstrap were used to test the significance of connections. The results showed that Granger causal connectivity existed from frontal to occipital and from parietal to occipital in attentional condition, while causal connectivity from frontal to occipital disappeared in unattentional condition.
Algorithms ; Attention ; Brain ; physiology ; Electroencephalography ; Healthy Volunteers ; Humans

International League Against Epilepsy announced the new International Classification of Epilepsy in 2001. One of the main objectives of the project is to adopt standard terms to describe seizure phenomena according to the terminological database. The project may profit academic communication and resource integration, and provide evidence for the individual treatment for epilepsy. Treatment based on syndrome differentiation is one of the characteristics of the treatment for epilepsy in traditional Chinese medicine (TCM). As far as the individuation on diagnosis and treatment is concerned, TCM and Western medicine present to reach the same goal by different routes gradually for the treatment of epilepsy. But for TCM, the diagnosis and treatment criteria for epilepsy are imperfect and the experts, opinions are not unified, so that the treatment based on syndrome differentiation becomes confused, hence the credit of treatment based on TCM is restricted accordingly. It is necessary to formulate advanced diagnosis and treatment criteria for syndromes of epilepsy in TCM.

0.05) none of the 39 cases of small cell type was in the AB blood group