1.Diagnostic value of Reid Scores under colposcopy in cervical human papiliomavirus infection
Journal of Shanghai Jiaotong University(medical Science) 2010;30(2):171-173
Objective To explore the diagnostic value of Reid Scores under colposcopy in cervical human papillomavirus (HPV)infection. Methods Cervical biopsy under colposcopy was carried out for cervical diseases in 528 patients.With pathological findings as criteria,the value of Reid Scores under colposcopy in diagnosis of HPV infection was analysed.Results The coincidence of Reid Scores under colposcopy in diagnosis of HPV infection and cervical intra-epithelial neoplasia(CIN)was 52.66%,with the sensitivity of 51.05%in diagnosis of low-grade cervical diseases(HPV infection and CIN I)and that of 66.67%in diagnosis of high-grade cervical diseases(CIN Ⅱ and CIN Ⅲ).For the patients with diagnosis of HPV infection examined by pathology,the scores of items of boundary,color and vessels were positively related to the total Reid Score,while the scores of items of boundary and color were not related to that of vessels. Conclusion There may exist clinical significance in diagnosis of HPV infection by colposcopy in combination with RCI Scores.
2.Insulin resistance in chronic kidney disease patient treated with glucocorticoids
Ping ZENG ; Jianling TAO ; Xuemei LI
Chinese Journal of Clinical Nutrition 2012;20(3):166-172
Glucocorticoids have remained important anti-inflammatory agents in the treatment of chronic kidney disease (CKD).However,the use of glucocorticoids can invoke or enhance insulin resistance,which is closely associated with renal injury and serves as an independent risk of the occurrence and progression of CKD.On the other hand,CKD patients may have insulin resistance,which hampers the use of glucocorticoids in these patients.
3.Effect of Astilbin on expression of CTLA-4 in activated T cells of mouse heart transplantation model with acute rejection
Si-Hai GAO ; Ping LI ; Tao CHEN ;
Chinese Journal of Organ Transplantation 2005;0(10):-
Objective To investigate the effect of astilbin on expression of CTLA-4 in activated T cells of mouse heart transplantation model with acute rejection.Methods Cardiomyocytes of BALB/ c mouse and spleen cells of C57BL/6 mouse were separated.The cardiomyocytes(2?10~5/ml)as irri- tation cells and spleen cells(1?10~6/ml)as responsers were mixed and cultured.The model of mouse heart transplantation with acute rejection in vitro was established.Three groups were set up:control group,Astilbin(15/?g/ml) group,Astilbin+anti-CTLA-4 mAb 9H10 (30?g/ml)group.Apoptosis of T cells was observed by TUNEL.The expression of CTLA-4 in activated T cells was detected by RT-PCR and Western blot.Results Apoptosis indexes of activated T cells in Astilbin group were sig- nificantly higher than those in the control group(73.4%?12.5% vs 35.1%?9.2%,P<0.01). The expression of CTLA-4 in Astilbin group was significantly higher than control group(P<0.01), but there was no apparently difference between control group and Astilbin+CTLA-4 mAb group(P>0.05).Conclusion Astilbin induces apoptosis of activated T cells in heart transplantation,which may be partially related to its enhanced expression of CTLA-4.
4.Oral-appliance for erectile dysfunction induced by obstructive sleep apnea-hypopnea syndrome.
Tao ZHANG ; Jing LI ; Ping YANG
National Journal of Andrology 2014;20(6):510-513
OBJECTIVETo evaluate the effect of the oral-appliance in the treatment of ED induced by obstructive sleep apnea-hypopnea syndrome (OSAHS).
METHODSThis study included 40 cases of OSAHS-induced ED. Based on the apnea-hyponea index (AHI) and lowest SaO2, we divided the patients into groups A (AHI < or = 30/h and lowest SaO2 > or = 80%) and B (AHI > 30/h and lowest SaO2 < 80%), and treated them with the oral-appliance for 3 months, followed by evaluation of the therapeutic effect using polysomnography, IIEF-5 questionnaire and Sexual Encounter Profile (SEP) diaries.
RESULTSThirty-seven of the patients accomplished the treatment, 16 in group A and 21 in group B. Compared with the baseline, the AHI was significantly decreased in both groups A (21.4 +/- 7.3 vs 14.2 +/- 6.6, P < 0.05) and B (51.4 +/- 9.5 vs 29.7 +/- 8.1, P < 0.05) after treatment, the IIEF-5 scores increased, even more remarkably in B than in A (3.36 +/- 2.48 vs 1.12 +/- 1.27, P < 0.05). The positive rates of SEP2 and SEP3 were markedly lower in A than in B (37.5% vs 76.2% and 25.0% vs 61.9%, P < 0.05).
CONCLUSIONThe oral-appliance can improve the erectile function of OSAHS-induced ED patients, particularly for those with severe OSAHS.
Adult ; Aged ; Erectile Dysfunction ; complications ; therapy ; Humans ; Male ; Middle Aged ; Orthodontic Appliances ; Sleep Apnea, Obstructive ; complications ; therapy ; Treatment Outcome
5.Laparoscopic appendectomy in 368 cases
Tao JIANG ; Xukun ZHOU ; Ping LI
Chinese Journal of Minimally Invasive Surgery 2001;0(03):-
Objective To summarize the experience of laparoscopic appendectomy(LA). Methods A retrospective analysis was made on clinical date of 368 cases of appendicitis treated with laparoscopic appendectomy in this hospital from March 1998 to March 2005.There were 35 cases of simple appendicitis,276 cases of suppurative appendicitis,39 cases of appendiceal perforation with peritonitis,and 18 cases of chronic appendicitis. Results The procedure was successfully accomplished in 363 cases,while conversions to open surgery were performed in 5 cases.The operative time was 22~55 min(mean,30.5 min),and the intraoperative blood loss was 2~10 ml.One case of abdominal residual infection and 1 case of adhesive ileus were cured with conservative treatment postoperatively.Pelvic abscess occurred in 1 case and was cured with a re-operation of laparoscopic abscess drainage. Conclusions Laparoscopic appendectomy is safe and effective in skilled hands.
7.Saccharide mapping and its application in quality control of polysaccharides from Chinese medicines.
Shao-ping LI ; Ding-tao WU ; Jing ZHAO
China Journal of Chinese Materia Medica 2015;40(17):3505-3513
Polysaccharides with multiple biological activities are usually considered as one of the major bioactive compounds in Chinese medicines (CMs). At present, the development of drug and functional foods related to polysaccharides have attracted a great deal of attention due to their great potential effects and diverse action mechanisms. However, quality control of polysaccharides is the bottleneck and a challenge due to their complexity and chemical diversity. Actually, the bioactivities of polysaccharides are closely related to their molecular structures. In order to ensure their safety and efficacy, the development of novel approaches based on the molecular structures for the improvement of quality control of polysaccharides is significantly important. Therefore, in this article, the relationship between biological activities and chemical structures, as well as the action mechanisms of polysaccharides from CMs were summarized first. Furthermore, saccharide mapping, a novel strategy for quality control of bioactive polysaccharides from CMs, was introduced and the application and perspectives were also discussed.
Carbohydrate Sequence
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Drugs, Chinese Herbal
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chemistry
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Molecular Sequence Data
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Polysaccharides
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chemistry
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Quality Control
8.Analysis on Application Rationality of Anti-fungal Agents for Deep Fungal Infection in Inpatients in a Top Three Hospital
Ping WANG ; Xiujie LI ; Tao YIN ; Min JIN
China Pharmacist 2014;(2):290-292
Objective:To investigate the rationality of anti-fungal agents for deep fungal infection used in one hospital and the effects of national rectification of antimicrobial drugs. Methods: The retrospective analysis method was used to survey the inpatients administrated anti-fungal agents for deep fungal infection from April 2010 to Mard 2012, and assessed the rationality. Results:The ir-rational utilization of anti-fungal agents for deep fungal infection included loading dosage lack during the treatment,inappropriate loading dosage and administration frequency. The irrational utilization of anti-fungal agents for deep fungal infection was decreased significantly (P<0. 05)after the national rectification of antimicrobial drugs. Conclusion:After the national rectification of antimicrobial drugs, the hospital can amtrol the irrational use of anti-fungal agents to some extent, while still needs more management and education.
9.Advances in actinobacterial proteomics.
Yao ZHANG ; Ping XU ; Wenjun LI ; Yong TAO
Chinese Journal of Biotechnology 2014;30(7):1044-1058
Protein is the executor of physiological function, and direct embodiment of the life phenomena. Proteomics aims to systematically clarify all or parts of proteins' role and function in life movement. In post genome era, proteomics began to play more important role in life science field. Actinobacteria are closely linked to human production and life, which have produced many clinically important secondary metabolites, including antibiotics, antitumorals and enzymes. Actinobacterial systematics and its model organism Streptomyces coelicolor in 2001 genome sequence laid the foundation for further functional genomic studies. Actinobacterial proteomics was more directly and exactly to interpret the activity of life than genomics and transcriptomics, which grew much faster and received so much attention from scientists in the near years. Complex morphological differention, stronge environment adaptiveness, nitrogen-fixing capacity, metabolic mechanism, pathogenicity and natural produces' discovery were systematically reviewed in this study, which was expected to be the basis for promoting Actinobacterial proteomics study in the near future.
Actinobacteria
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genetics
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metabolism
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Genomics
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Proteomics
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Streptomyces coelicolor
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genetics
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metabolism
10.Analysis of missed diagnosis of acute kidney injury in non-nephrological hospitalized adult patients
Ping CHEN ; Yun ZHOU ; Min MAO ; Rongshan LI ; Tao WEN
Chinese Journal of Nephrology 2014;30(9):645-649
Objective To raise the awareness of acute kidney injury (AKI) and improve the level of diagnosis.Methods All the hospitalized adult patients in the Affiliated People's Hospital of Shanxi Medical University from January 2012 to July 2013 were screened.Those patients diagnosed as AKI were retrospectively analyzed in terms of incidence,the rate of missed diagnosis,etiology,distribution and prognosis of AKI.Results (1) The incidence of AKI in the patients was 0.67% (381/56 835),the ratio of male to female was 1.91 ∶ 1,and the average age was (63.45±16.95) years.(2)There were 321 cases diagnosed as pre-renal AKI (84.25%),42 cases diagnosed as renal AKI (11.02%) and 18 cases diagnosed as post-renal AKI (4.72%).(3)There were 189 cases(49.61%) missed diagnosed among all the screened cases.The rates of missed diagnosis in neurosurgery,general surgery and cardiac surgery department was 62.96%,59.09% and 50.00%,in cardiology,respiratory and neurology department was 50.00%,50.00% and 45.45% respectively.(4) Multivariate Logistic regression analysis showed that hypertension [odds ratio(OR)=1.631],renal replacement therapy(RRT)(OR=23.256) and oliguria history (OR=1.936) were independent risk factors of missed AKI diagnosis.Conclusion The missed diagnosis rate of AKI is high and has certain characteristics in different departments.Hypertension,RRT and oliguria history are independent impact factors of missed AKI diagnosis.