In order to probe into the mechanism of low-dose chemotherapeutic agents in the treatment of leukemia, we studied the effects of low concentrations (l-40ng/ml) of daunorubicin (DNR) on proliferation and differentiation of human promyelocytic leukemia cell line (HL-60). 1 ng/ml DNR could inhibit the proliferation of the cells, but no induction of differentiation was found. DNR at more than 5 ng/ml had both cytotoxicity and induction of differentiation. Through the count of survival cells, [3H]TdR and [3H]UR incorporation, cloning efficiency, cell morphology and NBT reduction test, it is concluded that low-dose DNR could have inhibitory and cytotoxic effects on the cells, and its inducing differentiation was not important.

The effects of 1, 25 (OH)2D3 in combination with low-dose chemotherapeutant on the human promyelocytic leukemic cells (HL-60) were investigated. In 4-day-cultured cells, 1, 25 (OH)2D3 and low-dose harringtonine (HH) or daunorubicine (DNR) had synergic effect in inducing differentiation of HL-60 cells, and HH or DNR significantly potentiated the effect of 1, 25 (OH)2D3 in inhibiting the cell proliferation, which were demonstrated by NBT reduction test and [3H]-TdR incorporation, respectively. Cell cycle analysis by flow cytometery revealed that the cells in S phase decreased remarkably and most cells stopped at G0/G1 phase. The results provide a new way for the inducers in treatment of leukemia.

The effect of inhibition of proliferation of a novel human megakaryoblastic leukemia cell line (HIMeg) by two physiological factors, 1.25-dihydroxyvitamin D3 [1,25(OH)2 D3] and 13-cis-retinoic acid (RA), was investigated. At the range of 10-9 - 10-6 mol/L, 1,25(OH)2 D3 and RA showed significant inhibition of proliferation of the megakaryoblastic leukemia cells,which was demonstrated by count of survival cells,incorporation of [3H]-TdR and [3H]-UR, and cloning efficiency. From above, it can further explain the m.echanism of differentiation-inducers and the effect of 1,25(OH)2D3 on myelofibrosis. It is possible for 1,25(OH)2D3 and RA to be used for a new -treatment.

Recently, LC-MS/MS-based molecular networking has been widely used in the field of natural products from microorganisms, marine organisms, fungi, plants, and so on, while there are few reports on the research and application of LC-MS/MS-based molecular networking in the field of Chinese materia medica (CMM). Covering molecular networking topic, what is molecular networking was firstly introduced and the literatures related to molecular networking was retrieved. Then the application of molecular networking on five aspects were reviewed, including qualitative characterization of components, quantitative characterization of components and quality control, guiding the active compounds separation, optimizing the extraction process and disease diagnosis as well as personalized treatment. In the end, this study forecasted the prospects of molecular networking in the field of CMM, aiming to provide new thought and methodology for the research of CMM and expect to expedite the development of traditional Chinese medicine.

Humans ; Organophosphorus Compounds ; analysis ; pharmacokinetics

Objective To explore the method and effect of prosthetic replacement for the management of intertrochanteric fracture in the elderly.Methods 24 elderly patients with intertrochanteric fracture were treated with prosthetic replacement.According to the Evans classification,there were 4 cases of Evans Ⅱ,16 Evans Ⅲ and 4 Evans Ⅳ.Results The mean time to ambulation following the surgery was (6.0 ± 2.2) days.No prosthetic loosening or infection were observed in the follow-up of 6 to 27 months(average 12 months).According to the Harris hip score,the excellent and good rate was 83.3％.Conclusion Prosthetic replacement is safe and effective for intertrochanteric fractures in elderly patients,which allows early ambulation and have a relatively low rate of complications.

Objective To explore the method and effect of treating thoracolumbar fracture with posterior bone grafts on first stage and transpedicular internal fixation .Methods The clinical data of 22 cases with thoraco-lumbar fracture ,treated with posterior bone grafts on first stage and transpedicular internal fixation at the same time , were analyzed and assessed .Results In this group,the vertebral height and physiological curve were recovered satis-factorily,no loss of which was found in the long-term follow-up.There were no loosening of internal fixation ,infection and iatrogenic nerve injury .All patients had achieved bone fusion in one year after the operation of posterior bone grafts,and their nerve functions were recovered .Conclusion The operation of transpedicular internal fixation is safe and reliable ,and is proved to be an effective method of treatment for patients with thoracolumbar fracture .

Thermostable L-arabinose isomerase (L-AI) is the most potential enzyme for the biological production of D-tagatose from D-galactose, a novel functional factor. Gene araA encoding the L-arabinose isomerase from Bacillus stearothermophilis IAM 11001 was cloned and expressed in Escherichia coli. The araA gene of 1491 bp has 95% identity with L-AI from Thermus sp. IM6501. The GenBank accession number for the nucleotide sequence of this araA gene determined in this work is EU394214.The bacterium was induced by IPTG and analyzed by SDS-PAGE, approximately 59 kDa exogenous protein was observed on the SDS-PAGE. The recombinant L-AI was purified to electrophoretical homogeneity with affinity chromatography, and the activity of recombinant L-AI was also studied. The bioconversion rate of D-galactose to D-tagatose reached 39.4% after 24h whole cell reaction.

L-arabinose isomerase (L-AI) can isomerize L-arabinose and D-galactose into L-ribulose and D-tagatose, respectively, which is currently the most effective biological catalyst for D-tagatose production. The crystal structure of L-AI has been solved recently and its gene has been cloned, sequenced and overex- pressed. L-AI improved by protein engineering will be the dominant enzyme for industrial production of D-tagatose. This paper reviewed researches on protein structure and function, properties and application in D-tagatose production of L-AI, and the long-term potential development of L-AI was prospected.

Objective To investigate the effects of surgery-induced menopause on neuropsychological symptoms in women with perimenopause. Methods Sixty-eight women with perimenopause who underwent bilateral oophorectomy for benign indications were evaluated by the modified Kupperman index on the perimenopause syndrome pre-and post-surgery,including the score in neuropsychological symptoms.Patients were further requested to remake a decision on the acceptance of surgery,and were divided into "accept the surgery" group(n=30) and "postpone the surgery" group(n=38).The neuropsychological symptoms were reevaluated and comparison was made between these two groups. Results The prevalence of perimenopause syndrome pre-surgery was 67.65%(22/68),and that of moderate and severe symptoms was 30.88%.The prevalence of perimenopause syndrome post-surery(97.06%)(66/68)was significantly increased,as well as that of moderate and severe symptoms(70.59%)(P