Objective Observing the effect of general anesthesia (GA) with remifentanil on parturients undergoing cesarean section and Apgar score of child.Methods 40 parturients were divided into two groups,GA combined remifentanil (group A,n =20) and standard GA(group B,n =20).Observe the change of hemodynamics parameter and Apgar score of child.Results In group A than in group B maternal hemodynamic index smooth,two groups of intubation after lmin SBP,DBP,HR decreased,while the5 min after intubation returned to normal.SBP DBP,HR before induction,the difference between groups was not statistically significant (P＞ 0.05),the two group after induction,SBP,DBP,HR intubation after 1 min were (120.8 ± 19.6 mm Hg,80.6 ± 11.2 mm Hg,98.1 ± 17.1 BPM),(128.1 ± 17.5 mm Hg,98.9 ± 13.8mm Hg,108.7 ± 20.7 BPM) after intubation of 5 min were (98.3 ± 24.7 mm Hg 75.3 ± 13.8 mm Hg,79.9 ±22.6 BPM),(121.5 ± 16.2 mm Hg,88.6 ± 13.7 mm Hg,110.5 ± 17.4 BPM) had a significant difference (P ＜ 0.05),neonatal 1 min,5 min Apgar score,no significant differences between the two groups (P ＞0.05).Conclusion When remifentanil is used in the general anesthesia,its inducing is smoothly and it is no significant bad effect on the newborn.

Objective To study the feasibility of laparoscopic procedures in the treatment of ectopic pregnancy with hemorrhagic shock. Methods Laparoscopic procedures were carried out in 35 patients with ectopic pregnancy concurrent with hemorrhagic shock. Patients' blood pressures were all above 60/30 mmHg, and B-ultrasonography revealed the intra-abdominal blood loss

Objective To investigate the regulatory effect of transforming growth factor-? 1 (TGF-? 1)on the proliferation and apoptosis of human prostatic epithelial cells in vitro. Methods The primary culture was prepared by collagenase dissociation of minced prostatic tissue obtained from 8 BPH patients (53 to 70 of age) on open prostatectomy and from 5 organ donors (23 to 36).Cells were cultured in serum free prostate epithelial cell growth media and identified by immunocytochemical studies.The cells were passaged and cultured in media free of bovine pituitary extract and EGF and TGF-? 1 and EGF in different doses were then added either alone or in combination according to experimental requirement.The rate of cell proliferation was assessed by counting the cell number and by 5-bromo-2'-deoxyuridine (ELISA).Apoptosis induced by TGF-? 1 was examined by in situ transferase-mediated dUTP nick and labeling. Results The cultured cells had the typical morphological features of epithelium and stained positive for PSA and Pan-Keratin.The cells from normal prostates of young donors showed more mitogenic activity than those from BPH prostates.EGF enhanced cell proliferation whereas TGF-? 1 inhibited cell proliferation which could be reversed by increasing concentration of EGF.TGF-? 1 induced apoptosis of human prostatic epithelial cells.The effect of TGF-? 1 and EGF was concentration dependent. Conclusions TGF-? 1 is a direct physiological regulator of cell proliferation and cell apoptosis of human prostatic epithelial cells.

Objective To investigate the effect of TGF ? 1 and EGF on the proliferation of cultured human prostatic epithelial cells. Methods The primary culture was prepared by collagenase digestion of minced prostatic tissue obtained from prostatectomies of 8 benign prostatic hyperplasia(BPH) patients (aged 53 to 70) and 5 organ donors (aged 23 to 36). Cells were cultured in serum free prostatic epithelial cell growth media and identified by immunocytochemical method. The cells were passaged and cultured in media without bovine pituitary extract (BPE) and then, EGF and TGF ? 1 with different concentration were added into the culture either alone or in combination according to the experimental protocol. The cell proliferation was assessed by counting the cell number (BrdU) and ELISA. Results Cultured cells showed typical morphological features of epithelium and stained positive for PSA and Pan Keratin. After 5 7 passages cells went senescence. BPE and EGF enhanced whereas TGF ? 1 inhibited the cell proliferation and the inhibition was reversed by increasing the concentrations of EGF. The effect of both TGF ? 1 and EGF displayed concentration dependent manner. Conclusions Cell proliferation of the cultured human prostatic epithelial cells could be modulated by TGF ? 1 and EGF. These results indicated that TGF ? 1 and EGF, as the negative and positive growth factors might play an important role in the pathogenesis of BPH.

The incidence of the lung cancer increased as the aging of population. Lung cancer in the aged characterized in feature and treatment. This paper discussed the characteristics, early features, the problems of routine treatment, the alternative managements such as green therapy, assessment and improvement of quality of life, etc., in old lung cancer.

To improve the precision of image registration based on corner detection, a relative position function between multiple points to determine matching points accurately. First the corners in images are detected using Harris detector, and clustering method is used to eliminate most wrong matches after coarse screening. Then the proposed relative position function is used as a criterion of precise matching. Finally the image registration process is accomplished by affine transformation. Results show that the proposed algorithm is more effective and accurate than conventional registration algorithm.

Objective: To study the diagnosis and treatment of primary hyperparathyroidism.Methods: The clinical data of 8 primary hyperparathyroidism patients(1992 2002) in our hospital were summarized and the references were reviewed.Results: It was found that primary hyperparathyroidism was liable to be misdiagnosed.Information on blood calcium,parathyroid hormone(PTH) and imaging may be helpful for diagnosis. Surgical resection(4 cases), anhydrous alcohol injection (2 cases), electron radiation (1 case) and internal medicine (1 case) were applied. Conclusion:Information on blood calcium,PTH and early imaging is helpful for diagnosis.Operation is the first choice of treatment, and for patients who can not tolerate surgery, anhydrous alcohol injection is an effective and safe treatment.

Objective: Surfactant protein A (SP-A) is protein that appears to play an important role in mammalian first-line host defense. The objective of this study was to immunolocalize SP-A in human sinonasal tissue. Method:Eleven cases of allergic rhinitis, fifteen cases of polyp and seven cases of normal middle turbinate were studied with immunohistochemistry and immunofluorescence method to detect the expression of SP-A. Result:The expression of SP-A in allergic rhinitis and polyp were dramatically higher than that in controls(P<0.05), and there was no remarkable difference in the expression of SP-A between allergic rhinitis and polyp(P>0.05). The result demonstrated that SP-A was positivly correlated with eosinophils within the basement membrane of epitheli-um(R=0.81,0.55). In the result of immunofluorescence, there was significantly higher expression SP-A in nasal mucosa of allergic rhinitis and nasal polyp than that in control group(P<0.05). Conclusion:SP-A is likely to play key roles in the inflammatory reaction process of allergic rhinitis and polyp. Its secretion in the upper airway indicates that future studies may allow manipulation of this protein and development of novel treatments for sinonasal pathology.

Objective To study the characteristics of injuries in patients,and provide basic evidence for injury prevention and control.Methods 3 hospitals were sampled by stratified sampling,and the data of all injured patients visiting the 3 hospitals in 2006～2007 were analyzed.Results 11 142 injured patients were collected.The ratio of male to female was 1.81∶1.The average age was 34.56?16.Injured patients were mainly peasants(40.04%) and students(20.62%).Most injuries occurred at home and highway,and at 9～10AM and 3～4PM.The cause of injuries were fall(32.12%),traffic accidents(20.33%) blunt force(16.60%),and animal attacks(11.67%).Unintentionally injured patients accounted for 87.70% of all cases.Conclusions The occupation injury,traffic injury and injury to young adolescents are seriously endangering the health of peasants and students.

Objective To evaluate the application effect of clinical pharmacy services in the secondary prevention ot stroke.Methods Hospitalized stroke patients were selected in Taihe Hospital from June 2013 to August 2014.The patients meeting the inclusion criteria were paired and sequentially numbered,and randomly divided into observation group (n =100) and control group (n =102).The patients were treated routinely during thc hospitalization.The clinical pharmacists conducted the drug education.The control group implemented the telephone follow-up for guiding compliance behavior one month after discharge.The observation group continued to perform clinical pharmacist-leading service projects.The medication compliance (Morisky scale),systolic blood pressure (SBP),diastolic blood pressure (DBP),glycated hemoglobin (HbA1 C),serum total cholesterol (TC),low density lipoprotein-C (LDL-C) and relapse rate were compared three months,six months and twelve months after the two groups discharged.Results The medication compliance,SBP,DBP,HbA1C,TC,LDL-C and recurrence rate after hospital discharge were no statistically significant (P ＞ 0.05) three months after the two groups discharged,but statistically significant between the two groups (P ＜ 0.05 or P ＜ 0.01) six months and twelvc months after the two groups discharged.Levels of SBP,DBP,HbA1 C,TC and LDL-C in the two groups were both significantly lower than before (P ＜ 0.05 or P ＜ 0.01).The relapse rate of the observation group three months,six months and twelve months after they discharged was 2.00％,5.00％ and 15.00％,respectively,and that of the control group was 2.94％,13.72％ and 28.43％,respectively.Conclusion The pharmacy services in the secondary prevention of stroke can improve the patient medication compliance and better control the blood pressure,blood glucose and blood lipid lcvcls,and reduce the relapse rate.It should be recommended in the clinical practice work.