Objective To investigate the cytocompatibility of the liching liquids of Co-Cr alloy and Ti alloy on human gingival fibroblasts (HGF). Methods The HGF were treated in vitro with leaching liquids of Co-Cr alloy and Ti alloy, respectively. The DMEM cell medium containing 10% fetal bovine serum was served as a negative control. The viability of HGF treated by two dental alloys were evaluated by means of MTT, and the contents of intracellular reduced glutathione (rGSH) were assayed by kits. The tumor necrosis factor-α(TNF-α) contents were determined in the culture supernatant by ELISA in two groups. The effects of these alloys on the expression of caspase-3 were examined by real time-PCR method. Results Compared with the control group, HGF treated with Co-Cr alloy leaching liquids showed a lower viability ( P ＜0.05), while Ti alloy leaching liquid promoted the proliferation of HGF. In Co-Cr alloy group, the rGSH content was significantly decreased (P＜0.05), while TNF-α content was significantly increased (P＜0.05) compared with control group. There were no significant differences in rGSH and TNF-α contents between the Ti alloy group and control group (P＞0.05). The expression of caspase-3 was significantly higher in Co-Cr alloy group than that of control group (P＜0.05), while there was no significant difference in the expression of caspase-3 between Ti alloy group and control group. Conclusion Results suggest that Co-Cr alloy possesses cytotoxicity, while there is better cell compatibility for Ti alloy.

The paper suggests appling various teaching methods comprehensively to raise the students' interest and activate the class atmosphere to improve the biochemistry teaching effect on the basis of teaching materials and the level of the students during the teaching practice.

To observe the pathological changes of vascular aging in spontaneously hypertensive rats (SHRs), and the effects of extracts from Panax ginseng, Panax notoginseng (Burk.) and Ligusticum chuanxiong on vascular aging.

Objective To investigate the significance of detecting the level of CRP,TNF-α,IL-6 and IGF-1 in the patients with type 2 diabetes mellitus(T2DM).Methods Sixty patients with T2DM in the Pingliang Municipal People′s Hospital from February 2013 to March 2015 were selected as the observation group And contemporaneous 60 individuals undergoing healthy physical exami-nation were selected as the control group.The levels of FBG,2 hBG,FINS,HbA1c,CRP,TNF-α,IL-6 and IGF-1 were detected in these two groups.The correlation between CRP,TNF-α,IL-6 and IGF-1 with FBG,FINS and HbA1c were analyzed.Results The levels of FBG,2 hBG and HbA1c in the observation group were significantly higher than those in the control group,the difference was statistically significant (P<0.05).The FINS level in the observation group was significantly lower than that in the control group,the difference has statistically significant (P<0.05).The levels of CRP,TNF-α,IL-6 and IGF-1 in the observation group were (8.46±2.45)mg/L,(18.56±4.23)ng/L,(13.53±3.24)pg/L and (125.33±23.14)ng/L respectively,which were signifi-cantly higher than (3.23±1.02)mg/L,(11.25±3.01)ng/L,(7.24±1.78)pg/L and (98.45±18.56)ng/L in the control group, the difference was statistically significant(P<0.05).CRP,TNF-αand IL-6 were positively correlated with FBG,FINS and HbA1c;IGF-1 was negatively correlated with FBG and HbA1c.Conclusion Detecting the CRP,TNF-α,IL-6 and IGF-1 levels can provide a basis for the diagnosis of T2DM and thinking for its treatment.

Objective B-type natriuretic peptide(BNP) have been used widely in the diagnosis and prognosis of cardiogenic diseases, but the association between BNP concentrations and non-cardiogenic critical illness is unknown. Our study aims at investigating the role of natriuretic peptide testing for predicting 28-day mortality of non-cardiogenic critically ill patients in emergency department (ED). Methods A total of 255 non-cardiogenic critically ill patients treated in the emergency department were prospectively enrolled and a sample of blood was obtained for BNP measurement from each patient. A variety of clinical and laboratory variables were recorded. After 28 days, the vital status of each patient was ascertained and the association between BNP values at presentation and mortality was assessed. Results 71 patients died during the 28-day observation period. Median BNP concentrations at presentation among decedents were significantly higher than those of survivors (326. 0 ng/L versus 50. 9 ng/L,P <0. 001). The optimal BNP cut point for predicting 28-day mortality was 114. 0 ng/L. In a multivariate analysis (Cox-regression), a BNP concentration greater than 114. 0 ng/L(RR 7. 268, 95% CI 3. 864-13. 672) and an Acute Physiology and Chronic Health Evaluation Ⅱ (APACHE Ⅱ) scores greater than 20 (RR 3. 330, 95% CI 1. 815-6. 109)were the independent predictors of the 28-day mortality. BNP concentration alone had an area under the receiver operating characteristic curve (AUC) of 0. 825 for predicting mortality. Conclusions BNP concentrations at presentation are strong predictor of 28-day mortality in patients with non-cardiogenic critically ill patients in ED, which is better than Rapid Acute Physiology Score (RAPS) , Rapid Emergency Medicine Score (REMS) and APACHE Ⅱ score.

Objective To evaluate the value of uhrasonography in diagnosing the occult dorsal carpal ganglion. Methods Sixteen patients with chronic dorsal wrist pain received ultrasound examination. Eleven ofthem were treated with surgical therapy. And they were followed up for 1-8 months. Results The ultrasound characteristics of oecuh dorsal carpal ganglion were depicted as follows;①The dorsal cortical bone of scaphoid and lunate was continued and without damage. ② An oval anecho lesion with clear boundany, smooth ganglion wall, lateral shandowing and posterior enhancement could be detected in the dorsal part of scapholunate joint. ③Color Doppler flow imaging showed there was not obvious blood flow signals inside and around the ganglion. Conclusions Uhrasonography could be acted as a routine examination technique after the X-ray examintion in the diagnosis of the the occult dorsal carpal ganglion.

AIM:To observe the effect of phacoemulsification and intraocular lens ( IOL ) implantation combined with goniosynechialysis on corneal endothelial cells for the treatment of primary angle closure glaucoma ( PACG ) combined cataract, and to analyze the relative factors.METHODS: Ninety-five eyes of 95 patients with PACG combined cataract were documented in this study. Twenty-two patients were male, and 73 were female. The age ranged from 46 to 85y old with a mean of(66±7) y. All patients were examined for endothelial cell count ( ECC ) , intraocular pressure ( IOP ) and best corrected visual acuity ( BCVA ) 1wk, 1, 2, 3 and 6mo after operation. Meanwhile, the range of anterior chamber closure and anterior chamber depth ( ACD ) were recorded before operation and postoperative 6mo.
RESULTS: The mean IOP was 36. 1±4. 3mmHg ( 28-42mmHg) preoperatively and 15. 8±3. 5mmHg ( 8-28 mmHg)(1mmHg=0. 133kPa) 6mo after operation. There was a decreasing trend in IOP after operation( t=17. 173, P<0. 01). There was an increased trend in BCVA after operation(Z=-8. 351, P<0. 01). The mean ACD was 1. 95±0. 34mm preoperatively and 3. 11±0. 33mm 6mo after operation. There was an increased trend in ACD after operation(t=11. 483, P<0. 01). There were 74 eyes in the range from 90o to 180o of anterior chamber and 21 eyes less than 90o before operation. There were 12 eyes in the range from 90o to 180o of anterior chamber and 83 eyes less than 90o postoperatively. The most of anterior chamber were open(Z=-9. 013, P<0. 01). ECC was 2304±135 cells/mm2 before operation and 2 243±152 cells/mm2 , 2 135±177 cells/mm2 , 2 028±172 cells/mm2 , 2 017±181 cells/mm2 , 2 006±143 cells/mm2 1wk, 1, 2, 3 and 6mo after operation respectively. ECC loss rates were 2. 6%, 7. 3%, 12. 0%, 12. 5% and 13. 0%. ECC had a decreased trend 2mo after operation(F=5. 568, P<0. 01) and then kept stable(P3mo=0. 067, P6mo=0. 073).
CONCLUSION: Phacoemulsification and IOL -implantation combined with goniosynechialysis is an effective method to treat PACG combined cataract. It can increase BCVA and decrease IOP. ECC decreases after operation, but it is in the normal range. It is a safe and effective operation mode.

The accelerating pace of exchanges and cooperation in medical field between China and foreign countries demands a large number of medical papers, medical-related materials to be ac-curately translated into multiple languages , especially between English and Chinese . Oriented at English majors (medicine directed), based on eco-translatory approach and student-centered teaching method by creating simulations of the medical English ecological translation environment, this paper provided a better and effective practice-based teaching idea, which can promote students' medical English translation competence and strategies, even cultural consciousness and pragmatic ability in a comprehensive manner.

The causes of transient ischemic attack (TIA) are diverse and complex.It often attacks repeatedly and its early risk of stroke is high.Identifying etiology and pathogenesis and start the correct treatment in time are the important measures for the prevention of cerebral infarction.However,the conventional CT and MRI reveal no abnormalities in patients with TIA,and it brings difficulties to the clinical diagnosis and treatment.CT perfusion imaging can reflect abnormal cerebral blood flow perfusion in patients with TIA.CT perfusion imaging in combination with CT angiography may visually display its corresponding abnormal changes in the feeding artery and provide the valuable diagnostic information for the location of vascular stenosis,severity,plaque characteristics and collateral circulation.It has very an important significance for guiding clinicians to choose the best treatment options,improve clinic efficiency and observe efficacy.

10.3969/j.issn.1000-8179.2013.12.007