Objective To analyze the clinic and imaging characteristics as well as development of intervertebral disc calcification in children.Methods 6 cases underwent intervertebral disc calcification.X-ray films and CT scan of cervical spine had been taken in all cases,two cases had MRI examination.All cases were followed-up for 2 to 3 years by radiograph and CT.Results Only one intervertebral disc involved in all cases.X-ray and CT findings included disc calcification,disc herniation and change of vertebral boy in form.The clinical symptoms disappeared after conservative therapy 2 to 4 weeks.But the disc calcification disappeared until 6 months to 3 years.Conclusion Intervertebral disc calcification in children is a self-limiting disease with excellent prognosis,its diagnosis depends on imageology.

On the clinic,the main treatment of autoimmune diseases were used immunosuppressant,but large-scale use of immunosuppressive agents could cause of the delayed dysfunction,side effects such as infection,tumors and so on,so-induced immune suppression and immune tolerance response was the most effective way.So far,dendritic cells(DC) was the most powerful professional antigen-presenting cells.On one hand,DC as a full-time antigen-presenting cells and trigger regulation of the inherent and acquired immune response,start the immune response;On the other hand,DC through a variety of mechanisms induced antigen-specific T cells incompetent,show strong plasticity in immune tolerance and play an important role in immune regulation.Nuclear factor(NF)-?B is a nuclear transcription factor.In the immune response,NF-?B in the dendritic cells regulation of their own including development,maturity,the antigen-presenting,to stimulate T cells functions and so on.Such adjustments play an important role in the incidence of autommine diseases,could became a new target treatment of autommine diseases.

Objective To compare the effects of transcranial and trans-spinal low frequency magnetic stimulation (MS) on the expression of growth-associated protein-43 (GAP-43) in the spinal neurons of rats after spinal cord injury (SCI). Methods Forty-five Sprague-Dawley female rats were divided randomly into a transcranial magnetic stimulation (TMS) group,a trans-spinal cord magnetic stimulation group and a model control group with 15 in each group.Another 6 rats served as a normal control group.Spinal cord injury at the T9-10 level was modeled according to the modified Allen's method.The rats in the two MS groups received magnetic stimulation at 0.5 Hz and 75％ of the maximum output intensity ( 1.9 T) of the magnetic stimulator beginning 24 h post injury.They received 30 pulses daily for 28 days while the rats in the model control group were not exposed to MS.Basso-Beattie-Bresnahan (BBB) scale scores and motion evoked potentials (MEPs) were measured at the 1st,7th,14th,21 st and 28th day post injury.The expression of GAP-43 protein was studied using immunohistochemical methods and semi-quantified by Western blotting. Results At the 4th week post injury the average BBB scores in the model control group,the TMS group and the trans-spinal cord magnetic stimulation group were all significantly different. MEP amplitudes decreased significantly in all groups after the injury,and the latency was prolonged,but there were no significant differences among groups at any time point.GAP-43 expression also increased after SCI,and it was significantly higher in the MS groups than in the model control group at 4 weeks. Moreover,the expression of GAP-43 in the trans-spinal cord MS group was significantly greater than in the TMS group.Conclusion Magnetic stimulation can promote nerve regeneration and improve locomotor recovery after spinal cord injury,at least in rats.The effect of trans-spinal cord magnetic stimulation is superior to that of TMS.The mechanism may be related to the activation of central pattern generators.

Objective To explore the influence of late pregnancy complicating uterus myoma on the maternal and fetal out‐comes .Methods A total of 368 patients with late pregnancy complicating uterus myoma were taken as the observation subjects . The influence situation of size ,number and location of uterus myoma on the pregnancy ,delivery ,puerperium and maternal‐fatal out‐comes was analyzed .Results The incidence rate of threatened abortion ,threatened premature delivery ,abnormal fetal position ,pla‐centa praevia and placental abruption in complicating uterus myoma with diameter≥5 cm ,multiple ,intramural and cervial myoma was obviously higher than that in complicating uterus myoma with diameter<5 cm ,single and subserous myoma or submucous my‐oma (P<0 .05) .The cesarean section rate for the uterus myoma with diameter≥5 cm ,multiple and intramural myoma was signifi‐cantly increased (P<0 .05) .The incidence rate of fetal distress ,fetal anomaly ,premature infant ,low birth weight infant ,and neo‐natal asphyxia in uterus myoma with diameter≥5 cm ,multiple ,and intramural myoma was obviously increased(P<0 .05) .Conclu‐sion The late pregnancy complicating large ,multiple and intramural myoma has more obvious adverse reactions on maternal‐fetal prognosis .

Based on the research project of the French language teaching reform in Kunming Medical College——the Application and Analogy of Modern Foreign Languages Pedagogy,the successes and disadvantages of the visual-audio-oral course were studied.The teaching reform's purpose and focal points were primarily introduced.Then,the successful experiences of academic period arrangement,teaching materials selection,in-class instruction and students' academic achievements were elaborated.Finally,by making a comparison of students' French language proficiency between the present experimental classes and the former ordinary classes,a few disadvantages of the reform were interpreted.

Hospitals frequently end up losing medical lawsuits due to the following factors: inappropriate appointment of legal representatives, misconception and wrong application of the standard of proof required for lawsuits, ignorance of the time limit for submitting lawsuit documents, and lack of communication with the judges on the proof value of medical literature. In order to guarantee their legitimate rights and interests, hospitals ought to appoint health lawyers and medical experts as their legal representatives, have a good grasp of the standard of proof of reasonable probability, adhere to the time regulations in lawsuits, strengthen communication with the judges with regard to medicine, and understand the legal rules of arbitration. Only by doing so can their legal rights and interests avoid being encroached upon.

Objective:To analyze the changes of chemical compositions and relative contents of volatile constituents in raw Xanthi-um sibiricum Patr. and fried Xanthium sibiricum Patr. Methods: Headspace solid phase micro extraction ( HS-SPME) combined with gas chromatography-mass spectrometry ( GC-MS) was used to study the volatile components in raw and fried products of Xanthium sibir-icum Patr. . A peak area normalization method was applied to calculate the relative percentage content of each composition. Results:Totally 26 and 27 kinds of compounds were isolated and identified respectively from raw and fried xanthium including 19 species and re-spectively accounting for 68. 69% and 90. 08% of the total volatile components. In the raw products, the components with high con-tents were camphor (15. 3%), eucalyptus oil alcohol (9. 6%) and borneol (6. 89%), while in the fried products, those were men-thol (24. 09%), borneol (18. 48%) and eucalyptol (7. 19%). Conclusion:The relative contents of components with effects on rhi-nitis in the fried products are obviously higher than those in the raw products. HS-SPME-GC-MS can be used to detect the volatile com-positions and their relative contents in the raw products and fried products of Xanthium sibiricum Patr. . From the changes of volatile components, theoretical basis can be laid for the components with certain efficacy in raw and fried Xanthium sibiricum Patr. , and scien-tific basis can be provided for their further study and comprehensive utilization.

Objective:To compare and analyze the volatile components in different medicinal parts of silktree Albizzia julibrissin and their relative contents .Methods:Chromatography-mass spectrometry using headspace solid phase microextraction combined with gas method ( HS-SPME-GC-MS) was used to analyze the volatile components in the bark and flowers of silktree Albizzia julibrissin and the relative contents were calculated by a peak area normalization method .Results:In the bark and flowers of silktree Albizzia julibris-sin, totally 30 and 33 compounds were respectively identified , which respectively accounted for 73.20%and 88.29%of the total vola-tile.A total of 15 components, including alkanes, alkenes, aldehydes, furans, alcohols and acids were in the bark and flowers of silk-tree Albizzia julibrissin, among them, the content of cineole was the highest (bark:15.02%and flowers:21.9%).Conclusion:The HS-SPME technique can be used to analyze and compare the volatile components in the bark and flowers of silktree Albizzia julibrissin, which provides scientific basis for the reasonable development and application of the bark and flowers of silktree Albizzia julibrissin.

Objective To evaluate the usefulness of combined three-dimensional (3D) and two-dimensional (2D) contrast enhanced magnetic resonance angiography (CE-MRA) for checking the thrombus embolism of different positions within single examination on the pulmonary artery and pelvic-lower limb deep veins. Methods Fifteen patients with suspected pulmonary embolism and pelvic-lower limb deep venous thrombosis (DVT)were evaluated with combined 3D MRA and 2D CE-MRA. 3D spoiled gradient-recalled-echo bolus chase MR angiograms were obtained in four stations from the pulmonary artery to the ankle. Thereafter,3D CE MRA was reversely scanned from the ankle to the pelvic. 2D contrast-enhanced MRI was obtained in pelvis, thigh, and calf. Pulmonary CT angiography (CTA) and/or DSA were performed in 15 patients,and duplex ultrasonography of lower-limb vein was performed in 12 patients. Results Of the 15 cases, acceptable imaging of pulmonary vessel was acquired with 3D CE-MRA in 12 cases. The signal intensity was lower in the deep iliac vein and lower extremities than that in the artery, but vein frame was distinct after post processing. The artery and deep vein were clearly revealed with contrast enhanced FSPGR sequence in 15 cases. 3D CE-MRA imaging disclosed pulmonary embolism in fourteen patients and pelvis-lower limb DVT with multi-place involvement in nine patients. 2D contrast-enhanced MR imaging proved DVT in pelvis-lower limb. 2D contrast-enhanced FSPGR sequence was a complementation of 3D CE-MRA and it had larger scan field. Thrombus presented as low signals and eccentral or intraluminal filling defect. Local caliber of vein thrombus in 6 cases was evidently broadened. Conclusion Within only one MR examination procedure, it is capable of examining the pulmonary embolism and DVT of pelvis-lower limb with combined 3D MRA and 2D contrast enhanced MR. The results are promising as a non-invasion "one-stop shopping" tool in the evaluation of thromboembolic disease.

Objective To evaluate the performance of the Beckman Coulter ACT-5DIFF AL automated hematology analyzer and to verify whether it meets the clinical requirement.Methods The residual contamination rate,accuracy,precision,uncertainty,measurement range,reference interval,and sample injection pattern of detecting system were evaluated.Results The residual contamination of each parameter was less than or equal to 0.18％.According to room between qualitative evaluation results,compared to the target value,bias ranged from 0.32％ to 2.29％.Different concentrations of laboratory variation coefficient (namely precision) of each parameter ranged from 0.35％ to 4.46％,and both of which were less than a third of the CLIA'88 ability verification analysis quality requirements.The expanded uncertainty of each parameter was Uwhite blood cell (WBC) low =7.4％,UWBC high =3.8％,Ured blood cell (RBC) low =3.4 ％,U RBC high =2.8 ％,Uhemoglobin (HGB) low =3.9 ％,UHGB high =2.2 ％,Uplatelet (PLT) low =9.8 ％,UPLT high =7.6％,UMCV low =2.6％,and UMCV high =2.5％.Analysis had a wide measuring range:WBC (0.2 ～ 137.3) × 109/L,RBC(0.72 ～ 7.66) × 1012/L,HGB (20 ～ 231)g/L,PLT(25 ～983) × 109/L,and hematocrit (HCT) (6.1 ～68.0)％.All of them had a linear relationship,and the correlation coefficient of linear regression was close to 1.0.The reference interval quoted was suitable.Both of the automatic and the hand sample injection pattern had no significantly difference in result detection.Five categories of WBC were verified up to standard.Conclusions Under the circumstance of indoor quality control approved,each performance indicator approximately reached the laboratory quality requirements,and it also met the clinical requirements.