Objective To investigate the indication, methods and therapeutic effects of breast-conserving surgery in mammary cancer of early stage. Methods A retrospective study on 28 cases of Ⅰ and Ⅱ stage of breast-conserving surgery was underwent. Results Breast-conserving surgery does not destroy the appearance symmetry of breast, and maintains a low baldness rate. The recurrence rate in 5 years after surgery is 5.3%, and the 5 years' survival rate is 100%. Conclusion Breast-conserving surgery combined with the hinese herbal medicine therapy is an effective and safe way to treat mammary cancer in a early stage.

[Objective]To introduce micro-trauma technic with small incision treating the vertical fractures of the sacrum by intrasacral rod and evaluate the preliminary clinical outcomes.[Method]A total of 13 cases(9 males and 4 females) were treated with micro-trauma technic by intrasacral rod.The age ranged from 18 to 63 years,mean 36 years.Of all,6 cases were treated with anterior pelvis fixation,7 cases had sacral plexus injury.[Result]The operation lasted for 40～75 minutes(averaged 54 minutes) and hospitalisation period ranged from 8 to 20 days(averaged 11 days).A follow up lasted 9～24 months(averaged 17.5 months) in 12 patients.Ten cases were free from sacroiliac pain,2 cases had a slight pain. In 7 sacral plexus injury patients,6 cases completely recovered.[Conclusion]Micro-trauma technic with small incision treating the vertical fractures of the sacrum by intrasacral rod is a safe and effective method and has the advantages of minor trauma,less complication,short operation time and quick recovery.

Objective To assess the impact of recombinant pigment epithelium-derived factor (rPEDF) on the growth and invasion of the glioma cells.Methods The level of vascular enthothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) were evaluated by enzyme-linked immunoadsorbent assay (ELISA); cell proliferation assays were performed by MTT assay; the cell migratory assay was used for evaluating its inhibitory migration rate.Results The VEGF expression (0.29?0.03) of A172_ PEDF (added the PEDF to the A_ 172 cells) decreased 4.4 times compared with the A172_ con (not added the PEDF to the A_ 172 cells, 1.31?0.02, P

0.998 8).The minimum detection limit were 0.1～0.4 mg?L-1.The average recovery rates were no less than 96%(RSD≤7.19%).There were significant difference among the concentration of Ara-C and Ara-U in plasma,cerebrospinal fluid and testicular tissue.Ara-C in plasma hadn't been determined in low-dose group and medium-dose group except for Ara-U.CONCLUSIONS: The method is simple,rapid,accurate and reproducible for plasma concentration monitoring and pharmacokinetic study of Ara-C and Ara-U.

Objective To establish a new and convenient assay for whole blood lead concentration. Methods Whole blood sample was digested by nitric acid-perchloric. Under the condition of pH 0.8～1.0, lead concentration was examined by Hydridegeneration Atomic Fluorescence Spectrometry.Results The method had good linearity when lead concentration were between 0 to 600 ?g/L, r =0.999. The within-run CV of the method for high, middle and low lead level were 4.1%, 3.6% and 3.5% respectively. The between-run CV were 4.9%, 4.6% and 4.7% respectively. The recovery rates were 105.4%, 98.5% and 94.8% respectively. The single-blind test result of series standard lead samples provided by Chinese Academy of Preventive Medicine were within the acceptable range. The detecting Limit of this method was 0.3 ng. There was no significant interference from main two-valence positiveion. The sample can be storage at lest for 1 months at 4℃. Samples of 869 children from Tianjin were tested by this method. The mean value was 91.9 ?g/L, median was 83.8 ?g/L, all data were within 23.1～386.3 ?g/L. The result showed out positive skewness distribution.Conclusion This method were sensitive, accurate, precise, quick and low-cost. It was suitable for use in clinical laboratories.

Objective To express the pigment epithelium-derived factor (PEDF) in E.coli. and to assess its role in angiogenesis of gliomas.Methods The expression plasmid pET41/PEDF was constructed and the protein was purified by metal affinity tag (His Tag ) and anion-exchange chromatography. Tube formation was used for evaluating its biological activity. The changes of vascular enthothelial growth factor (VEGF) and thrombospondin-1 (TSP-1) were evaluated by the enzyme-linked immunosorbent assay.Results The high purified and inhibitory-actived PEDF proteins were acquired and PEDF had the function of decreasing the VEGF 1.8 times as well as increasing the TSP-1 5.3 times.Conclusions It is possible that PEDF expression is a potent factor for the inhibition angiogenesis in gliomas.

Objective To study the therapeutic effects of Cordyceps polysaccharide (CP) on adenine-induced chronic renal failure(CRF)in rats. Methods CRF model was induced by Adenine ig .The rats were randomized into normal group, model group, CP high, moderate, Low-dosage groups and Dexamethasone group. The models of adenine-induced nephrosis were established by intragastric administration of adenine 300 mg/kg for 21 days. After treatment, renal function, biochemical indicators, kidney index, renal pathologic changes and serum TNF-? were observed. Results Compared with the model group, the degree injury of renal function in CP groups was palliated significant, renal pathologic changes were palliated significant, renal index and content of serum TNF-? were both decreased significant. Conclusion CP has a therapeutic and preventive effect on adenine-induced CRF, and can prevent the generation and development of CRF, improve renal function.

According to the correlative analyses on Chinese medicine essence, dosage forms and quality control level, it expounds the precise concept of Chinese medicine, and its quality advantages and characteristics in this paper, furthermore discusses how to achieve the ideal drugs and Chinese medicine quality precision in expectation. Base on the Chinese medicine essence, using the concept of nature medicine and its drug system to construct Chinese medicine effective material basis and its drugs, with the correlative analyses of whole view and reductionism, the problems of uncertainty quality of original natural medicinal resources and preparations may well be solved, and further with the macroscopic to microcosmic construction of drug system, the precision in expectations of Chinese medicine quality and higher production lever may well be achieved.
Drugs, Chinese Herbal ; chemistry ; pharmacology ; standards ; Humans ; Medicine, Chinese Traditional ; Plants, Medicinal ; chemistry ; Precision Medicine ; Quality Control

Objective:To evaluate the effects of different denture cleaners on the discoloration of heat-cured denture base resin and artificial teeth.Methods:40 same specifications of the heat-curing denture base resin and 40 artificial central incisors were immerced in an acombination stain of coffee,tea and soy sauce for 4 weeks.Then specimens and artificial teeth were randomly distributed into 4 groups and soaked in Polident,Steradent,0.5％ sodium hypochlorite solution and distilled water for 4 hours respectivelly(n =10).Color differences(△E) were measured by using a colorimeter and a denture spectrophotometer before and after staining,and after cleaning.Results:Before or after staining there was no difference of △E among the groups of denture base risn or artificial teeth(P ＞ 0.05).After cleaning the denture base resin and the artificial teeth in the group of 0.5％ sodium hypochlorite solution presented higher △E than the other groups(P ＜ 0.05),in group of distilled water presented lower △E than the other groups(P ＜ 0.05).No significant difference was found between Polident group and Steradent group(P ＞0.05).The △E of the denture base resin and artificial teeth.in distilled water group before staining and after cleaning were the highest among the groups(P ＜0.05).Conclusion:0.5％ sodium hypochlorite solution,Polident and Steradent are effective in removing the discoloration from the heat-cured acrylic resin and artificial teeth.0.5％ sodium hypochlorite solution is the most effective,Polident and Steradent are the similar.

AimTo establish the model of adriamycin- induced nephritic syndrome in rats and its pathological change wasobserved. MethodsThe model of nephritic syndrome in rats was established by intravenous injection of adriamycin in the dose of 5mg · kg-1 and 3 mg · kg-1 respectively at the first day and 15 th day. The amount of urine protein was measured at the 1、2、3、4、6、8 and 12 th week and the serum biochemical pa rameters of TG, TCH, BUN, Cr, TP and ALB were detected at the 6 th and 12 th week. Results The urine protein and the serum biochemic parameters were increased significantly, except the TP and ALB, which were decreased. Pathological changes at the sixth week was similar to minimal changes in glomerulonephritis, and at the 12 th week, sclerosis of glomeruli of kidney could be found. ConclusionAfter first injection,the changes of kidney were like those inminimal change glomerulonephritis of human being. But after the second injection. The kidney had the changes of focal segmental glomeralosclerosis.