Objective To investigate the expression of stromal cell-derived factor-1(SDF-1)and its clinical significance in blood plasma of patients with breast tumor.Methods The level of SDF-1 protein was examined by enzyme linked immunosorbent assay(ELISA)in blood plasma of 26 patients with breast benign tumor and 52 patients with breast cancer.Results The SDF-1 protein in blood plasma was detected in both breast benign tumor patients and breast cancer ones.The level of SDF-1 protein in patients with breast cancer was higher than that in ones with breast benign tumor,and there was a statistical difference between them(P=0.000).In patients with breast cancer,the level of SDF-1 protein in axillary lymph node(ALN)metastasis positive patients was significantly higher than that in ALN metastasis negative ones(P=0.036).Conclusion The level of SDF-1 protein in blood plasma may be a specific tumor marker.Its level is correlated with lymph node involvement in breast cancer.

The recipe-analgesic compound decoction composed of herbs according to the theory of Traditional Chinese Medicine and the author's clinical experience of treating painful diseases. The experimental studies showed that the pain threshold of mice in hot-plate test was raised (p

green light(480 nm).

Objective To investigate the use of chitin derivatives-carboxylation chitosan immediate separation feasibility of PLT preparation,and to look for a new direction in separation feasibility of blood components.Methods 40 samples of blood donors were divided into the experimental group,natural sedimentation control group and centrifugal control group randomLy in Dalian.2 mL of whole blood were mixed with different concentrations of carboxylation chitosan which were diluted by blood preservation solution Ⅱ by according to the ratio of 1 ∶4.plasma precipitation amount were surveyed after 4 hours,with numbers of red blood cells,white blood cell and platelet,PLT aggregation and the changes of red blood cell morphology were observed.Finally,suitable amount of MAP were added into the optimal chitosan in preservation,and hemolysis of red blood cells were tested in 35 days.Results Suitable amount of chitin experimental group blood sedimentation rate were significantly faster than static device control group,and plasma remaining trace red blood cells,PLT-rich,and 35 days no obvious hemolysis.Conclusion carboxylation chitosan could be used in PLT preparation.

Objective To study a wavelet method for acquiring the characters of pulse wave based on the principle of wavelet transform which can effectively solve the problem of inaccuracy of the conventional methods. Method The method of wavelet module maximum was used to divide the pulse wave according to periods and basically decomposed it, by arranging in time order and symbolizing the module maximum character points of pulse wave signal at the ascending and descending edges as well as the crest and trough. Result Through the method, the unobvious dicrotism, trail wave and the anomalistic wave appearing at the ascending and descending edge of the main wave, and multi-scale characters and all kinds of characters in time domain of pulse signal were further acquired more accurately. Conclusion Simplicity, quickness and accuracy are achieved by focusing on the character points of pulse signals. It provides a new means for further studies of classification and identification of pulse signals.

Adolescent ; Adult ; Child ; Female ; Follow-Up Studies ; Hematopoietic Stem Cell Transplantation ; Humans ; Leukemia ; therapy ; Male ; Middle Aged ; Prognosis ; Young Adult

objective To observe the cyclooxygenase-2(COX-2)expression and the cell proliferation and apoptosis of gastric adenocarcinoma cells after transiently transfecting gastric cancer cells using specific COX-2 small interfering RNA(siRNA)and discuss the role of COX-2 in gastric tumorigenesis and the effect of RNA interference(RNAi)as anti-cancer gene therapy.Methods Three groups were included in the study,i.e.a COX-2 siRNA group,a non-sense siRNA group and a control group.Gastric adenocarcinoma cells SGC7901 were cuhured at 37℃ in an atmosphere containing 5%CO2.72 hours after transfecting SGC7901 cells with specific COX-2 siRNA or non-sense siRNA,RT-PCR was used to detect COX-2 mRNA,immunohistochemistry and Western blot were taken to detect the expression of COX-2 protein and flow cytometry was taken to detect the cell cycle and apoptosis.MTT method was used to detect the proliferation and activity of the cells every day for one week after transfection.Results The expression of COX-2 mRNA and protein in the COX-2 siRNA group was obviously suppressed as compared with non-sense siRNA group and control group.No change was found between the non-sense siRNA group and the control group.The reduced expression of COX-2 could inhibit SGC7901 cells proliferation and induce cell apoptosis,but had no effect on cancer cell cycle.Conclusions The experimental results suggest that effectively inhibiting the expression of COX-2 can suppress the proliferation of gastric adenocarcinoma cell and promote the process of cancer cell apoptosis.so RNAi is a powerful tool of gene therapeutic method.

Tumor angiogenesis is the uncontrolled growth of blood vessels in tumors,serving to supply nutrients and oxygen,and remove metabolic wastes.Kaposi's sarcoma (KS),a multifocal angioproliferative disorder characterized by spindle cell proliferation,neo-angiogenesis,inflammation,and edema,is associated with infection by Kaposi's sarcoma-associated herpesvirus (KSHV).Recent studies indicate that KSHV infection directly promotes angiogenesis and inflammation through an autocrine and paracrine mechanism by inducing pro-angiogenic and pro-inflammatory cytokines.Many of these cytokines are also expressed in KS lesions,implicating a direct role of KSI-IV in the pathogenesis of this malignancy.Several KSHV genes are involved in KSHV-induced angiogenesis.These studies have provided insights into the pathogenesis of KS,and identified potential therapeutic targets for this malignancy.

Objective To explore the vitamin D nutritional status of urban residents in Jinan,in order to provide the basis for the prevention and treatment of chronic diseases associated with vitamin D insufficiency.Methods 1478 cases aged 30-90 years,including 602 men (59.8± 13.0) years on average and 876 women (57.4±12.9) years on average were selected by a stratified random sampling from 3 community medical care institutions in Jinan.Subjects were divided into six groups according to the 10-year interval.Serum 25-hydroxyvitamin D [25(OH) D] level was measured by enzyme-linked immunosorbent assay (ELISA).Serum [25(OH) D] level ≥ 75 nmol/L was defined as vitamin D sufficient,(50.0 ～ 74.9) nmol/L as vitamin D critical value,25.0 ～ 49.9 nmol/L as vitamin D insufficiency,＜ 25 nmol/L as vitamin D deficiency.All data processing and statistical analysis were finished by SPSS 13.0.Results The average level of [25 (OH) D] was 58.60 nmol/L in males [95％ CI:56.7-60.5 nmol/L] and 54.17 nmol/L in females [95％ CI:52.8-55.8 nmol/L],which were less than 75 nmol/L.The average level of [[25(OH) D] was 49.1nmol/L in females aged 70-79 years (95％CI:45.7～52.5 nmol/L),41.7 nmol/L in females aged 80-90 years (95％CI:38.2 ～46.7 nmol/L),which were less than 50 nmol/L.The percentage of cases with vitamin D deficiency,insufficiency,critical value,and sufficiency was 5.6％,33.4％,36.5％,24.4％ in males,and 6.5％,42.9％,32.6％,18.1％ in female respectively.The incidence of vitamin D deficiency and insufficiency was increased with ageing (male:x2 =33.68 P＜0.01,female:x2 =55.7,P＜0.001).The percentage of vitamin D deficiency and insufficiency was more in females than in males (60-69 years old:x2=9.387,P＜0.05,80-90 years old:x2=8.896,P＜0.05).The [25 (OH) D] level was lowest in winter (48.8 nmol/L,95％CI:46.8～50.9 nmol/L) and highest in autumn (60.2 nmol/L,95％ CI:58.0～62.3 nmol/L).The incidence of vitamin D deficiency and insufficiency was highest in winter (x2 =18.364,P＜0.05).Conclusions Vitamin D deficiency and insufficiency are widely prevalent in urban residents,which is more severe in the elderly and females.Serum [25(OH) D] level is related to season,and the vitamin D deficiency and insufficiency are severe in winter.

Objective To investigate the efficacy and safety of laparoscopic splenectomy (LS) in the treatment of immune throm-bocytopenia(ITP) .Methods 230 cases of ITP were collected in this hospital from December 2011 to December 2012 and randomly divided into two groups with 115 cases in each group .Among them ,the control group was treated by the conventional treatment of open splenectomy(OS) ,while the observation group was treated by LS .The differences of the operative time ,hospitalization dura-tion ,intraoperative blood loss and platelet count ,clinical effects and the occurrence situation of adverse reactions were compared be-tween the two kinds of operation method .Results The operative time and the length of hospital stay in the observation group were significantly shortened compared with the control group (t=14 .706 ,t=13 .625 ,P<0 .05);intraoperative blood loss was significant-ly decreased(t=18 .238 ,P<0 .05) and the platelet count was significantly increased (t=17 .248 ,P<0 .05);the total effective rate of the observation group was 96 .52% ,which was significantly higher than 86 .09% of the control group ,(χ2 =9 .35 ,P<0 .05);the adverse reaction rate and the recurrence rate in the observation group were 6 .09% and 6 .96% respectively ,which were significantly lower than 10 .43% and 19 .13% in the control group(χ2 =8 .35 ,χ2 =8 .75 ,P<0 .05) .Conclusion LS for treating immune throm-bocytopenia has definite efficacy with high effective rate ,low adverse reactions and low recurrence rate .