High-throughput screening is a regular approach available for identitying new lead compounds for the growing validated drug targets in drug screening. However, it has also introduced a large number of peculiar molecules which interfere drug screening. Pan assay interference compounds (PAINS) interfere with the progress of drug screening in various ways, such as interfering with a biochemical assay, modifying the protein, aggregate-based inhibitors and so on. So it is of vital significance to remove them. This paper has consulted the concept, category of PAINS and reviewed the way of PAINS interfering and the countermeasures to cope with them to direct the approach of high through screening and improve the hits percent.

Type-B RAF (BRAF) gene is one of the most popular genes of thyroid carcinoma in recent studies,and its mutation has significant relationships with the occurrence,development,treatment,and prognosis of papillary thyroid carcinoma(PTC).The influence of BRAFV600E mutation on the expression of iodine uptake relative proteins in PTC cells and the value of molecular targeted therapy with BRAFV600Einhibitors in the clinical treatment of PTC in the future were summarized in this review.

According to the national residency training regulations in the teaching of clinical and scientific research, the department of thoracic surgery of First Affiliated Hospital of Chongqing Medical University has used seminar teaching , and has collected random sampling survey questionnaire of 110 students participating in seminar from September 2014 to March 2016. The investigation shows that through seminar teaching training, doctors will cultivate their clinical and research ability, and enhance their self-confidence. Therefore, the application of seminar teaching in department of thoracic surgery is helpful to improving the quality of clinical teaching and is worthy of further improvement and promotion.

High-throughput screening is a regular approach available for identitying new lead compounds for the growing validated drug targets in drug screening. However, it has also introduced a large number of peculiar molecules which interfere drug screening. Pan assay interference compounds (PAINS) interfere with the progress of drug screening in various ways, such as interfering with a biochemical assay, modifying the protein, aggregate-based inhibitors and so on. So it is of vital significance to remove them. This paper has consulted the concept, category of PAINS and reviewed the way of PAINS interfering and the countermeasures to cope with them to direct the approach of high through screening and improve the hits percent.
Drug Discovery ; High-Throughput Screening Assays ; methods

Abnormal eye blinking in children is a disease of frequent eye blinking in children, more than 15 times per minute, without facial spasms and neurological diseases, with relatively independent clinical symptoms. If combining with organic or neurological disease, we consider it as children eye blinking syndrome. It's a common and frequently encountered disease in pediatric ophthalmology. The etiologies include body and psychological disorders, such as refractive errors, ocular surface and ocular adnexal disease, bad habits, lead pollution, tic disorders and so on. We think that most of abnormal eye blinking in children firstly caused by oculopathy, neurological disorders and psychological illness are the important factors for increasing or making it repeatedly happen. Recognizing and identifying the etiologies of abnormal eye blinking in children, so as to take a targeted theray and avoid misdiagnosis and delayed treatment.

Objective To treat arteriosclerosis patients with a simple self-made oxygenation respirator in a re-respiration model. Methods 43 AS patients was enrolled in treatment group(Oxygenation Respirator only) and control group (drug therapy only). Results the efficacy was 92%, better than control group, the efficacy of which was 72%, and P

China ; Industry ; standards ; Occupational Health Services ; standards ; Reference Standards

Objective:To assess the accuracy of the portable blood glucose meters. Methods:The study design was equivalent clinical trial. The participants were selected according to the inclusion and exclusion criteria from inpatients, outpatients and outpatient volunteers of the department of endocrinology. The fingertip blood glucose was measured by five portable blood glucose meters and compared with vein blood glucose measured by biochemistry. The measurement error was assessed by the mean difference with biochemical results. The correlation coefficient was calculated to test the correlation between fingertip and vein blood glucose. Results:there were 230 cases in total including 86 males and 144females. The average age was (50.6±13.5) years old. There were 73 fasting blood glucose and 157 postprandial blood glucose. The range of biochemical blood glucose value was from 2.19 to 19.95 mmol/L and the averaged biochemical blood glucose concentration was (6.77±2.65) mmol/L. The minimum measurement error was 0.08mmol/L measured by blood glucose meter C. However, there was one meter E whose 95%confidence interval of measurement error exceeds the equivalent interval (-0.83, 0.83) mmol/L range and the difference does not meet the clinical equivalence. The measurement bias was all lower than 95%. Conclusion:The blood glucose meters can be used as the day-to-day monitoring of blood glucose in patients. The accuracy needs to be improved and continuously monitored.

ObjectiveTo study the clinical effect of endoscopic treatment for acute non-variceal upper gastrointestinal bleeding.MethodsThe clinical data of 78 cases in our hospital with acute non-variceal upper gastrointestinal bleeding were retrospectively analyze.All patients were checked by gastroscopy accompanied by endoscopic treatment.Another 50 cases received ogilvy pull thiazole treatment were analyzed.ResultsAfter endoscopic therapy,all patients were successfully achieved hemostasis,success rate 100.0％.Of which 27 cases were treated by spraying drugs in conducting hemostasis,25 cases succeeded at the first time,the other two turned to injection after the failure;21 cases were treated by injection to stanch bleeding succeeded for the first time;30 cases were treated with titanium clips made it at the first course.None of the patients transferred to surgical treatment,no complications such as perforation and no death.Curative effect of Imidazole group compared with ogilvy pull is different with,a statistical significance( P ＜ 0.05 ).ConclusionEndoscopic treatment for acute non-variceal upper gastrointestinal bleeding could be affirmed,and relatively safe,little trauma,and it could be used as the first method in treating non-variceal upper gastrointestinal bleeding.

Objective The medication scope of 581 prescriptions contained in the book of Two hundred one party governance inspection record is studied through data mining.Methods After regulating drug names individually according to the authoritative literature,the statistical range of medication is given using the software Excel 2003.Results A total of 360 kinds of medicines are obtained.Conclusion The spectrum of 581 optimized ancient prescriptions is formed,which is the focus of our further research.