Objective:To study the expression of vascular endothelial growth factor (VEGF) and VEGF mRNA after focal cerebral ischemia reperfusion in rats.Methods:MCAO (Middle Cerebral Artery Occlusion) models were set up in rats,and the rats were divided into six groups:control group,5 groups of ischemia for 2h then respective reperfusion for 6h,12h,24h,48h and 72h.The samples were analyzed with immunohistochemical methods.The expression of VEGF mRNA was detected by RT-PCR analysis.Results:VEGF protein expression started at 6h.It increased remarkably at 24h,and it was strongest at 48h.VEGF mRNA increased at 6h,while reached peak value at 12h and reduced at 24h,finally returned to normal level at 48-72h.VEGF expression was mainly detected in the periphery of infarcts at every timepoint.Conclusion:These findings demonstrate that the VEGF strong expression can be induced in focal cerebral ischemia reperfusion in vivo.The changes of VEGF levels suggest that VEGF has neuroprotective effect against cerebral ischemic injury.

Objective:To evaluate the body mass change in overweight and simple obesity patients 1 year after withdrawal of home-made sibutramine preparation.Methods: Seventy-five overweight or simple obesity patients were enrolled in this Ⅱ phase clinical trial of sibutramine,and they were divided into sibutramine group(n=43) and placebo group (n=32). The patients body mass, body fat rate, waist circumference, serum lipid, blood glucose, blood pressure and adverse effects were determined 12,24 and 48 weeks after withdrawal of sibutramine. Results: There were no significant differences in body mass,body mass index, body fat rate and waist circumference between sibutramine group and placebo group 48 weeks after withdrawal of sibutramine. For sibutramine group,there was no obvious difference before and after treatment, but there was significant difference between immediately and 48 weeks after withdrawal of sibutramine (P

There is a strong support for a role played by adiponectin in the function of ovary and placenta.There is evidence of direct effects of adiponectin on the late stages of folliculogenesis,and additive interactions of adiponectin with insulin and gonadotropins in inducing periovulatory changes in ovarian follicles.Adiponectin has demonstrated a potential effect on ovarian function as well as a possible effect on the formation of placenta,via multiple mechanisms,providing new strategies for the etiology investigation,prevention and treatment of reproductive and related metabolic diseases.

Objective To observe the clinical curative effect of Wumei Pill in the treatment of cold-heat complex diarrhea-predominant irritable bowel syndrome.Methods Modified Wumei Pill was adopted to treat 46 cases of cold-heat complex diarrhea predominant irritable bowel syndrome.Five main symptoms of stool frequency,stool shape and properties,abdominal pain and discomfort,bowel gurgling,vulnerable to the cold were observed.The symptom scores before the treatment and 1 week,2 weeks and 4 weeks after the treatment were evaluated.Results After the treatment of 1 week,2 weeks and 4 weeks,the overall efficiency was 58.70％(27/46),84.78％(39/46),and 97.83％(45/46) respectively,and there were significant differences in curative effect between the different treatment courses (between treatment of 2 weeks and 1 weekx2＝8.571,between treatment of 4 weeks and 2 weekx2=16.426,between treatment of 4 weeks and 1 week x2=35.622).Effective rate of symptoms after 4 weeks treatment were respectively:stool frequency 82.05％ (32/39),stool shape and properties 95.56％ (43/45),abdominal pain and discomfort 92.86％ (39/42),bowel gurgling 85.71％ (18/21),vulnerable to the cold 64.29％ (27/42),and there was obvious differences in effective percentage of five kinds of symptoms between treatment of 4 weeks and 1 week (Stool frequency x2 =7.123、stool shape and properties x2 =13.611、abdominal pain or discomfort x2 =7.721、bowel gurgling x2=10.096、vulnerable to the cold x2=10.769).Conclusion.The clinical effect of Wumei Pill for the treatment of cold-heat complex diarrhea predominant irritable bowel syndrome was defined.

Polycystic ovary syndrome (PCOS)is the most common cause of anovulatory infertility in women of reproductive age, exerting significant impacts on glucose and lipid metabolism, cardiovascular and endometrial deseases, as well as breast, skin, bone, and psychology fields. As a complex, multi-system of endocrine and metabolic disease, PCOS would be recognized as a component of metabolic syndrome in an overall consideration. The clinical treatment of PCOS is expected to turn simply regulating menstruation and ovulation adjustment into the combination of insulin resistance improvement with antihyperandrogen and ovulation treatment comprehensively.

OBJECTIVE:To establish a method for determination of Rifampin in human serum,and to individualize the medication for tuberculosis patients with liver damage.METHODS:Using d-nitrophenol as the internal standard,the serum samples were extracted with methanol.HPLC condition:analytical column:Lichrospher100(4.6?m?250mm),guard column:YWG-C 18 (?4mm?40mm),the mobile phase:methanol-acetonitril-0.04mol/L KH 2 PO 4 (31.5∶31.5∶37,V/V/V),the flow rate:1ml/min,the column temperature:40℃,the wavelength of UV detection:254nm.The serum concentrations were deter?mined at given time periods after patient taking an oral dose of450mg Rifampin,and need of dosage adjustment was judged.RESULTS:The chromatography was good and not interfered by the components of the serum.The dosage of Rifampin had obvious individual difference.After adjusting the dosage,the effect increased and ADRs decreased.CONCLUSION:This is a simple,rapid,accurate,sensitive and reliable method to determine the concentration of Rifampin with a wide concentration range.It is suitable for the analysis of individualized medication of Rifampin.

OBJECTIVE:To evaluate the effectiveness of TCM and antibiotics in the treatment of urinary tract infection(UTI) systematically.METHODS:According to the requirements of Cochrane systematic review,literatures were retrieved from CNKI and Wanfang database.A meta-analysis was performed on 18 literatures conforming to inclusion criteria.RESULTS:Compared with control group,the OR value of treatment group and subgroups including TCM group,TCM combined with antibiotics group,chronic UTI group and senile UTI group were 3.99,3.95,3.95,4.23,4.35,respectively.The 95%CI of those groups were(2.97,5.37),(2.80,5.80),(2.80,5.80),(2.61,6.83),(2.64,7.18).The OR value was of treatment group 0.25(95%CI:0.17,0.37;P

Objective To analyze the clinical efficacy of microsurgical clipping for the treatment of ruptured intracranial aneurysm in elderly patients.Methods 150 patients with ruptured intracranial aneurysms in our hospital from May 2009 to Apr 2013 were randomly divided into observation group and control group,with 75 cases in each group.The observation group was treated with microsurgical clipping,and the control group was treated with endovascular intervention.The efficacy,Glasgow outcome scale (GOS) and postoperative complications were compared between two groups.Results The success rate of microsurgical clipping was significantly higher in observation group than in the control group (96.0％ vs.78.7％,x2 =13.54,P＜0.05).The cure rate of GOS scale was significantly higher in observation group than in the control group (80％ vs.66.7％,x2 =4.525,P＜0.05).The incidence rate of postoperative complications was significantly lower in the observation group than in the control group (9.3％ vs.20.0％,x2 =4.58,P＜0.05).Conclusions Microsurgical clipping for the treatment of ruptured intracranial aneurysm has the high success rate and cure rate,with fewer complications and lower medical costs,and is worthy of promotion.

5-fluorouracil (5-Fu) as the most common adjuvant chemotherapy has been widely used for patients with colorectal cancer.As reported dihydropyrimidine dehydrogenase (DPD) has been known as an initial rate-limiting enzyme to catalyze approximately 80 ％ of administered dose of 5-Fu.However,patients with a partial or complete DPD deficiency because of the mutation of DPYD gene may suffer from diarrhea,stomatitis,hand-foot syndrome or neurotoxicity and even death.In this article,the application of DPYD gene screening in patients with colorectal cancer of current status and recent clinical application are reviewed.

Objective To explore the clinical effects and safety of ipratropium bromide combined with β receptor agonists in treatment of asthma in children. Methods The patients were divided into control group and therapy group randomly. On the basis of the treatment for asthma, the control group and therapy group were treated with ipratropium bromide, ipratropium bromide combined with salbutamol, respectively. Results After treatment, the clinical symptoms such as cough, suffocated feeling, cyanosis and dififcult breathing were improved on different degree for both of the two groups. The levels of lung function indexes including FVC、FEV 1、FEV 1% and PEF and the total effective rate in therapy group were signiifcantly higher than the control group. Besides, no signiifcant adverse effect was observed. Conclusion The ipratropium bromide combined with salbutamol can be used to relieve asthma in children effectively and synergistically. The bioavailability and the compliance of children are good.