1. Effect of glutathione on autologous fat graft survival of the rabbits
Liangliang WU ; Li MA ; Sijing JIANG ; Daping JIA ; Fang LIU ; Tangjun GAO ; Yu ZHAO
Chinese Journal of Plastic Surgery 2019;35(9):928-933
Objective:
To investigate the effect of adding glutathion(GSH) to tumescent solution on autologous fat graft survival.
Methods:
14 male and female New Zealand rabbits were divided into experimental group and control group randomly, 7 in each group. Experimental group: The donor areas of the rabbits were injected with 3 ml of tumescent solution with GSH. Control group: The donor areas of the rabbits were injected with 3 ml regular tumescent solution. DCFH-DA probe was used for fluorescent staining of harvested fat cells. Then stained fat cells were measured for the intracellular reactive oxygen species(ROS)content by fluorescence microplate. The grafts were harvested at 3 months after transplantation and assessed by general observation, volume measurement, wet weight measurement, HE staining for the number of fat cells, and CD34 immunohistochemical staining for the measurement of micro-vascular density. T test was performed by using SPSS 24.0.
Results:
The intracellular ROS content of harvested fat cells in experimental group was lower than that in control group, and the difference was statistically significant (