By using ~3H—TdR incorporation and CTLL—2 cell line,we observed effects of RU486,anantagonist of type Ⅱ glucocorticoid receptor,on dexamethasone inhibition of lymphocyte prolifer-ation and interleukin—2(IL—2)production in rat spleen.It was shown that RU486 obviouslyantagonized the inhibitory actions of dexamethasone on lymphocyte proliferation and IL—2 pro-duction.By means of dot hybridization and Northern blot analysis,effects of dexamethasone andRU486 on IL—2 gene expression were investigated in lymphocytes of rat spleen.The datademonstrated that dexamethasone markedly decreased IL—2 mRNA production,RU486 alonedidn't affect IL-2 mRNA levels,but obviously reversed dexamethasone-mediated downregala-tion of IL—2 mRNA production in ConA—activated lymphocytes.These results suggest thatthe above effects of dexamethasone may be mediated by glucocorticoid receptor in lymphocytesof rat spleen.

OBJECTIVETo observe clinical therapeutic effect of electroacupuncture combined with medicine on acute gouty arthritis and effect on blood uric acid content.

METHODSOne hundred cases of acute gouty arthritis were randomly divided into 3 groups, an electroacupuncture group treated by acupuncture at Zusanli (ST 36), Fenglong (ST 40) and Ashi points, a Western medicine group by oral administration of indometacin and benzbromarone, and a combined electroacupuncture and medicine group by electroacupuncture combined with the Western medicine. The changes of their blood uric acid levels before and after treatment were detected and their therapeutic effects were compared.

RESULTSThere was a significant difference among the three groups in clinical therapeutic effect (P < 0.05), and the effective rate was respectively 97.5%, 90.0% and 86.7% in the combined electroacupuncture and medicine group, the electroacupuncture group and the Western medicine group, the combined electroacupuncture and medicine group being significantly higher than the electroacupuncture group and the Western medicine group; after treatment, the blood uric acid level in all the 3 groups had significant change (P < 0.05), the blood uric acid in the combined electroacupuncture and medicine group had more obvious decrease as compared with those in the electroacupuncture group or the Western medicine group.

CONCLUSIONElectroacupuncture combined with the Western medicine has a definite therapeutic effect on acute gouty arthritis.

Acupuncture Therapy ; Administration, Oral ; Arthritis, Gouty ; therapy ; Combined Modality Therapy ; Electroacupuncture ; Humans

OBJECTIVETo probe a better therapy for chronic persistent asthma.

METHODSThirty-six cases of chronic persistent asthma were randomly divided into a beat-sensitive moxibustion group (17 cases) and a western medication group (19 cases). In heat-sensitive moxibustion group, the sensitization points between Feishu (BL 13) and Geshu (BL 17) were selected with heat-sensitive moxibustion; in western medication group, Seretide inhalant was applied. The patients of both groups were continuously treated for 3 months and the therapeutic effects were evaluated by clinical symptom scores and pulmonary ventilation function test.

RESULTSAfter treatment, the effective rate of 88.2% (15/17) in heat-sensitive moxibustion group was superior to that of 47.4% (9/19) in western medication group (P < 0.05); the clinical symptom scores and pulmonary ventilation function were obviously improved in the two groups, and the heat-sensitive moxibustion group was superior to the western medication group (all P < 0.05).

CONCLUSIONFor chronic persistent bronchial asthma, heat-sensitive moxibustion can improve the clinical symptoms and the pulmonary ventilation function, and it is better than Seretide inhalant.

Adolescent ; Adult ; Aged ; Albuterol ; analogs & derivatives ; therapeutic use ; Androstadienes ; therapeutic use ; Asthma ; drug therapy ; physiopathology ; therapy ; Chronic Disease ; therapy ; Drug Combinations ; Female ; Fluticasone-Salmeterol Drug Combination ; Humans ; Lung ; physiopathology ; Male ; Middle Aged ; Moxibustion ; Pulmonary Ventilation ; Treatment Outcome ; Young Adult

Objective To validate if the continuous passive motion instrument we developed is practical and sta- ble for animal experiment.Methods Ten New Zealand male rabbits were used to establish the animal model of full- thickness defect of articular cartilage in the facies patellaris of femur with their both knees.The rabbits were then randomly divided into groups A and B(10 in each group).The rabbits in group A were administered with CPM of both knees daily for 4 weeks,while those in group B remained normal cage activity only.At the end of the treatment,all the animals were sacrificed,and their articular cartilage was harvested for HE staining and observation.Results It was found that group A had a significantly better repair of the full-thickness defects of articular cartilage than that of the group B,as reflected by the range of motion and morphological observation of the knees(P

Objective To observe the change of stanniocalcin 1 (STC1) and calcium content in brain of coal-burning-borne fluorosis rats,and to explore the role of STC1 in brain injury of coal-burning-borne fluorosis.Methods Twenty four male SD rats were randomly divided into control,low,medium,and high fluoride groups according to body mass.Control group was fed conventional rat chow(fluorinated 1.3 mg/kg),and low,medium and high fluoride groups fed with fluorinated feed(20.0,40.0,60.0 mg/kg).All rats were given distilled water and feed ad libitum.One hundred and eighty days after modeling,STC1 protein and gene expression in the brain tissue of rats were detected using immunohistochemistry and RT-PCR and calcium content of brain tissue was detected.Results The cell positive rates of STC1 in low,medium,high fluoride groups [(48.10 + 2.11)％,(54.90 ± 1.73)％,(79.30 ± 3.71)％] were significantly higher than that of the control group[(24.70 + 3.53)％,all P ＜ 0.05],the cell positive rate of high fluoride group was significantly higher than that of the low and medium fluoride groups (all P ＜ 0.05).The STC1 mRNA expression of low,medium and high fluoride groups (0.58 ± 0.09,0.85 ± 0.17,1.75 ± 0.04) were significantly higher than that in the control group(0.37 ± 0.12,all P＜ 0.05),the STC1 mRNA expressions of high fluoride group was significantly higher than that of the low and medium fluoride groups (all P ＜ 0.05).The brain cortex calcium ion concentrations of low,medium and high fluoride groups[(138.62 + 4.19),(167.43 + 6.57),(189.45 + 3.72)nmol/L] were significantly higher than that in the control group [(101.47 + 9.46)nmol/L,all P ＜ 0.05],the brain cortex calcium ion concentrations of high fluoride group was significantly higher than that of the low and medium fluoride groups(all P ＜ 0.05),and the medium fluoride groups was higher than the low groups (P ＜ 0.05).Conclusion STC 1 may be involved in brain damage of coal-burning-borne fluorosis rats through regulating calcium balance.

OBJECTIVETo search for a new method for treatment of acute gouty arthritis.

METHODSSixty cases of acute gouty arthritis were randomly divided into a treatment group and a control group, 30 cases in each group. The treatment group were treated with electroacupuncture combined with point-injection, and the control group with western medicine. Their therapeutic effects were observed and serum uric acid contents were detected before and after treatment.

RESULTSThere was a very significantly difference between the two groups in the therapeutic effect (P<0. 01) and the analgesic effect in the treatment group was better than that in the control group (P<0. 01). The treatment group was superior to the control group in decrease of blood uric acid (P<0. 01).

CONCLUSIONElectroacupuncture combined with point-injection is an effective method for treatment of acute gouty arthritis, and it can decrease blood uric acid content.

Acupuncture Points ; Adult ; Aged ; Arthritis, Gouty ; therapy ; Electroacupuncture ; Female ; Humans ; Injections ; Male ; Middle Aged ; Uric Acid ; blood

OBJECTIVETo study the therapeutic effect of treatment of acute gouty arthritis (AGA) respectively by electroacupuncture (EA) with different frequency and oral intake of Western medicine.

METHODSSeventy-two patients of AGA were randomly assigned into three groups, 24 in each group. Group A was treated with EA 100 Hz; Group B with EA 2 Hz; and Group C with Western medicine. The analgesic effect, initiating time and sustaining time of analgesia were observed and the level of serum uric acid was measured before and after treatment.

RESULTSThe initiating time of analgesia was shorter while the sustaining time of analgesia was longer in Group A and B than those in Group C (all P < 0.01). The efficacy of analgesia was higher in Group B than that in Group A, and a better effect was shown in Group B in reducing serum uric acid level than that in Group A (P < 0.01), which was near that in Group C (P > 0.05).

CONCLUSIONEA is an effective treatment for AGA, and low frequency (2 Hz) EA showed a better efficacy.

Acupuncture Analgesia ; methods ; Acute Disease ; Adult ; Aged ; Arthritis, Gouty ; blood ; therapy ; Electroacupuncture ; methods ; Female ; Humans ; Male ; Middle Aged ; Pain ; blood ; Pain Management ; Treatment Outcome ; Uric Acid ; blood

OBJECTIVETo understand the distribution of diseases in inpatients from the department of acupuncture and moxibustion and to provide the references for the scientific research direction of acupuncture and moxibustion.

METHODSBy use of information management system of the medical records, the first page of the medical record of 6435 cases from January 1, 2003 to December 31, 2010 was extracted.

RESULTSIt shows a clear upward trend in the department of acupuncture and moxibustion in 8 years. The vast majority are elderly patients in 6435 cases, involving in 309 kinds of diseases, the major cases focus on the diseases of motor system and cerebrovascular system. The top 10 ranking of diseases and the rates are lumbar spondylosis (17.73%), cerebral infarction (13.77%), cervical spondylosis (13.66%), sequelae of cerebral infarction (7.47%), lumbar disc herniaion (4. 41%), osteoarthropathia (3.40%), cerebral hemorrhage (2.91%), herpes zoster (2.33%), postherpetic neuralgia (2.33%), sequelae of cerebral hemorrhage (2.32%).

CONCLUSIONThe people's acceptance of acupuncture and moxibustion is increasing and the sufferers treated by acupuncture and moxibustion are mostly elderly patients. The research on the motor system diseases and cerebrovascular diseases should be strengthened.

Acupuncture Therapy ; Adolescent ; Adult ; Aged ; Aged, 80 and over ; Child ; Child, Preschool ; Female ; Hospitalization ; statistics & numerical data ; Humans ; Inpatients ; statistics & numerical data ; Male ; Middle Aged ; Moxibustion ; Young Adult

OBJECTIVETo evaluate the oncological and functional outcomes in patients undergoing supracricoid partial laryngectomy (SCPL).

METHODSForty-three cases underwent supracricoid partial laryngectomy with functional reconstruction in stage T1b-T4. In these cases, 16 were supraglottic cancers, 21 were glottic cancers, and 2 were subglottic cancers. Three types of supracricoid partial laryngectomy (cricohyoidopexy CHP, cricohyoidoepiglottopexy CHEP and tracheocricohyoidoepiglottopexy TCHEP) were employed accordingly.

RESULTSThe 3- and 5-year accumulative survival rates were 90.7% and 83.7% respectively (Kaplan-Meier method). Median follow-up time was 57 months. Decannulation rate was 95.3% (41/43) in those. The mean time of decannulation was 14 days in patients preserved both cricoarytenoid units and 43 days in those only preserved one cricoarytenoid unit. The removal of epiglottis increased the risk of aspiration and prolonged time of decannulation (P < 0.05).

CONCLUSIONSSupracricoid partial laryngectomy for selected laryngeal cancer is feasible. The patients can gain satisfied survival rate and quality of life.

Aged ; Carcinoma, Squamous Cell ; mortality ; surgery ; Cricoid Cartilage ; surgery ; Female ; Humans ; Laryngeal Neoplasms ; mortality ; surgery ; Laryngectomy ; methods ; Male ; Middle Aged ; Retrospective Studies ; Survival Rate ; Treatment Outcome

OBJECTIVETo compare therapeutic effects of acupoint-injection and Chinese herbs for treatment of lumbago-leg pain induced by primary osteoporosis.

METHODSNinety cases of lumbago-leg pain induced by primary osteoporosis were randomly divided into a Jiaji point group, a reinforcing kidney and strengthening spleen point group and a medication group, 30 cases in each group. For the Jiaji point group, bilateral Jiaji points T11, L1, L3, L5 and S2 were selected; for the reinforcing kidney and strengthening spleen point group, Pishu (BL 20), Shenshu (BL 23), Taixi (KI 3), Taibai (SP 3), Taichong (LR 3), Sanyinjiao (SP 6), Xuehai (SP 10) were selected and the medication group were treated with oral administration of Qianggu Capsules. Changes of bone mass density (BMD) and pain cumulative score after treatment were observed.

RESULTSThe total effective rate was 93.3% in the Jiaji point group, 73.3% in the reinforcing kidney and strengthening spleen point group and 60.0% in the medication group, the Jiaji point group being better than both the reinforcing kidney and strengthening spleen point group and the medication group (P<0.01, P<0.05). In reduction of pain cumulative score and improvement of BMD, the Jiaji point group was better than both the reinforcing kidney and strengthening spleen point group and the medication group (P<0.01), and the reinforcing kidney and strengthening spleen point group was better than the medication group (P<0.05, P<0.01).

CONCLUSIONAcupoint-injection has a definite therapeutic effect on lumbago-leg pain induced by primary osteoporosis, and the therapeutic effect of the Jiaji point group is better than the reinforcing kidney and strengthening spleen point group.

Acupuncture Analgesia ; methods ; Acupuncture Points ; Aged ; Bone Density ; Female ; Humans ; Injections ; Leg ; Low Back Pain ; therapy ; Male ; Middle Aged ; Osteoporosis ; therapy