Objective To explore the relationship between Monoamine oxidase A (MAOA) receptor gene,catechol-O-methyltransferase (COMT) receptor gene,dopamine D3 receptor(DRD3) gene and 5-HT2C receptor gene(5-HT2c) of Han population in northern China and obsessive compulsive disorder.Methods Polymerase chain reaction amplification determination of MAOA-T1460C,COMT-Val158Met,DRD3-Ser9Gly,5-HT2c-C ys23Ser four loci receptor gene polymorphism in 164 patients with OCD patients including 103 core pedigrees of fragment length polymorphism,and association and linkage disequilibrium (TDT)analysis.Results There was no significant difference of MAOA-T1460C,COMT-Val158Met,DRD3-Ser9Gly,5-HT2c-Cys23Ser four receptor gene in the patient group and the control group of genotype and allele distribution difference(P＞0.05),four receptor gene loci were in accordance with the balance of the H-W,the MAOA-T1460C receptor gene in female patients group and control group,the early group and control group,which has forced thinking and difference of compulsive behavior group and the control group,only the obsessional group and the control group of genotype and allele distribution was statistically significant(P＜0.05),and the family group between chain(P=0.0001) ;5-HT2c-Cys23Ser receptor gene in the case group and the control group,male both forced thinking and compulsive behavior group and control group differences in genotype and allele distribution was statistically significant (P＜ 0.05),and between family groups exist chain (P=0.0389) ; COMT-Val158Met receptor gene in the control experiments were no significant difference(P＞0.05),and with the house group does not exist between the chain (P=0.0622) ;DRD3-Ser9Gly receptor gene in the control experiments were no significant difference(P＞0.05),and with the family groups there is no chain(P=0.1101).Conclusion MAOA-T1460C receptor gene polymorphism and 5-HT2c-Cys23Ser receptor gene polymorphisms may be the susceptible gene of obsessive compulsive disorder.

Objective To detect the effect of exogenous IL-6 on the proliferation of cholangiocarcinoma cell line QBC939.Methods MTT was used to observe the proliferation of cell line QBC939 treated with exogenous IL-6.Following the annexin V/FITC and PI staining,FCM was used to detect the apoptosis of QBC939 treated with exogenous IL-6.Results The proliferation of cell line QBC939 was increased after treatment with IL-6 and was positively related with the concentration of IL-6(P

Diabetic microangiopathy is one of the most common chronic diabetic complications,which is associated with the genetic predispositions and environmental factors.Well-controlled blood glucose alone can not reduce the morbility or prevent the process of diabetic microangiopathy.The therapy aiming at the pathogenesis is an urgent request.The various proteins related to the development and progression of diabetic microangiopathiy were reviewed,aiming at providing the potential target for early diagnosis and efficient therapy.

Objective:To assess the efficacy and safety of oncoplastic breast-conserving surgery (OBCS) in the treatment of early breast cancer. Methods:The clinicopathological data of breast cancer patients who were treated with OBCS (67 cases) and standard breast-conserving surgery (SBCS;117 cases) in Affiliated Cancer Hospital of Guangxi Medical University were retrospectively analyzed. Postop-erative complication, specimen weight, margins, and surgery re-excision rate between the two groups were compared. Results:Sero-ma (14.9%versus 48.7%, P<0.001), hematoma (4.5%versus 14.5%, P=0.035), and poor wound healing (3.0%versus 11.9%, P=0.036) were more common in the SBCS group than in the OBCS group. The patient satisfaction in the OBCS group was statistically higher than in the SBCS group (P<0.05). Compared with standard surgery, oncoplastic techniques can be employed for significantly larger tumors (25.04 mm versus 21.14 mm, P<0.001). OBCS resulted in higher mean specimen weights (92.24 g versus 57.44 g, P<0.001), wider clear nearest margins (12.04 mm versus 9.58 mm, P<0.001), and wider furthest margins (24.16 mm versus 15.24 mm, P<0.001). No statisti-cal increase was observed in further surgery re-excision of margins. Conclusion:OBCS is more successful than standard wide local exci-sion in treating larger tumors and obtaining wider radial margins. Oncoplastic approach showed no increase in postoperative complica-tion rate. The postoperative complication was excellent. OBCS is a safe and effective procedure for early breast cancer.

Cerebral microbleeds are new biomarkers of the cerebral small vascular disease.With the development of neuroimaging techniques,the related research of cerebral microbleeds has been paid more and more attention.The research of the correlation between cerebral microbleeds and cognitive impairment and stroke,imaging features,pathological changes,and clinical outcomes can provide an important basis for clinical treatment decisions.

Objective To evaluate the efficacy and safety of ultrasound-guided percutaneous ethanol injection(PEI)in the treatment of solitary benign thyroid adenoma.Methods One hundred and twelve patients with thyroid adenoma underwent PEI.The amount of anhydrous ethanol injected was approximately one third of the volume of thyroid nodule and used once a week,the injection was repeated two to five times.Results The average volume of pure adenoma and adenoma associated with cystic degeneration is ( 4.93 ? 0.48 )cm 3 and ( 7.89 ? 0.91 )cm 3;the nodules decreased markedly to ( 3.22 ? 0.40 )cm 3 and ( 3.85 ? 0.55 )cm 3 3 months after PEI treatment (P

A wide range of uncomfortable symptoms are attributed to menopause.It is also during this time that women are at increasing risk of poor medical conditions such as osteoporosis,cancers and dementia.Most physicians pay attention to the proper selection of effective and reasonable therapeutic methods,unfortunately,no agreement exists on the clinical recommendations and choice.The article reviewed hormone replacement therapy(HRT),common therapy and traditional medical therapy.

Objective: To study Bacterial Endotoxins Test(BET) for Herbe Houttuyniae Injection.Methods: The interfering test for Herbe Houttuyniae Injection was carried out. Results: The test wasn't interfered when TAL(0.25EU/ml) was used and sample was diluted in a ratio of 1 to 4.Conclusions: The BET may replace the rabbit pyrogen test.

Objective To study the content of taxol in different parts of Taxus Yunnanensis and the effects of different growth time on taxol content. Method A HPLC method was developed for the detetmination of taxol content in bark, needle, twig, root from Taxus Yunnanensi, and the effects of different growth time. Results Taxol content in bark was the highest, that in root was the lowest and that in needle and twig were higher. Taxol content was higher with the growth time in needle and twig of Taxus Yunnanensi. Conclution It was significant to take taxol from needle and twig of Taxus Yunnanensis which is more than five years growth time.

Objective To control the quality of Herba Leonuri by studying the content of stachydrine hydrochloride in different parts of it. Method A HPLC method was developed for the content detetmination of stachydrine hydrochloride in flower, stem, leafage from Herba Leonuri. Spherisorb SCX colum was used with mobile phase of 20 mmol/L sodium dihydrogen phosphate (containing 0.04% triethylamine and 0.15% phosphoric acid). The colum temperature was 25 ℃, the detective wavelength was 192 nm. Results Stachydrine hydrochloride content was the highest in leafage and the lowest in stem. Conclusion To ensure the quality of Herba Leonuri, it is significant to choose medical material with more leafage.