Objective To explore the levels of interferon ?(IFN-?) and interleukin-10(IL-10) in sera and in hepatitis B virus(HBV) core antigen(HBcAg) stimulated culture supernatants of peripheral blood mononuclear cells(PBMC) from chronic HBV carriers children and their clinical significance.Methods Five milliliter peripheral blood was collected from 21 HBeAg-positive children(group A),20 HBeAg-negative children(group B) and 19 normal controls(group C),sera and PBMC was isolated,after 72 h culture of PBMC with HBcAg or not.The levels of IFN-? and IL-10 in sera and in HBcAg stimulated or not stimulated culture supernatants of PBMC were detected.Results The level of sera IFN-? had no difference between each group;the sera level of IL-10 in group A and B were significantly higher than that of group C.The level of IFN-? in HBcAg stimulated culture supernatants of PBMC from group B and C were higher than that from group A;while IL-10 levels in HBcAg stimulated culture supernatants from group A and B were higher than that from group C.Conclusions The level of IL-10 and the lower reactivity to HBcAg of PBMC play roles in the mechanisms of chronic HBV carriers children.

Brain Diseases ; etiology ; Brain Neoplasms ; surgery ; Glioma ; surgery ; Humans ; Infection ; etiology ; Postoperative Complications ; etiology ; Recurrence ; Risk Factors

Through searching literature about cytochrome P450 from PubMed, CNKI, CBM and Wanfang database in the recent 10 years, this article concluded, summarized and analyzed vivo and vitro research methods of cytochrome P450 to provide references for the study on TCM cytochrome P450 enzyme.

OBJECTIVE To explore the pathogen distribution and their resistance in ICU of Geriatric hospital.METHODS 111 strains isolated from lower respiratory tract were collected from Sep 2006 to August 2007.The antibiotic resistance were tested.RESULTS Among 111 strains,G-strains accounted for 70.27%,in which Pseudomonas aeruginosa was the most common(20.72%).The G+bacteria were multi-resistant to most of the antibiotics except impenem.CONCLUSIONS The most common pathogens in ICU of geriatric hospital is G-bacteria with multi-resistance.The Antibiotics should be selected reasonably.

Objective To study the relationship between the microvesel density(MVD) ,vascular endothelial growth factor(VEGF) , matrix metalloproteinases 2 ( MMP2 ) with the metastasis, prognosis of cholangiocarcinoma. Methods MVD,expression of VEGF and MMP2 were investigated by means of immunohistochemistry in 45 cholangiocarcinoma tissues and 8 normal bile duct tissues. Results (1)MVD and positive expression rates of VEGF , MMP2 in cholangiocarcinoma tissues were significantly higher than those in normal bile duct tissues ( P

This article analyzes a series of ethical issues in the psychological health management of college students,puts forward some corresponding ethical countermeasures from the perspectives of reasonable objectives,principles,contents and operational process of the psychological health management on the basis of people-oriented and harmonious concept in order to correctly deal with ethical issues of psychological health management,and promote the development of psychological health management.

Objective To understand the status and developmental trends of the research on anterolateral thigh flap. Methods The PubMed search was downloaded and the results were imported into the EndNote database,then the distribution was analysed by age,nation,language,periodical and frequency.Results There were 236 articles on anterolateral thigh flap in PubMed from 1996 to August 2006.The amount of articles multiply increased from 2002 to August 2006,at eighty percent of all.They were published in 8 languages,in 47 journals and from 9 countries.China and the USA are the major countries of the publication,with 77 articles each,which altogether took up 65.2% of the total.Words of a high frequency are as follows: free skip flap,island skin flap,ultrathin skin flap,perforating artery,and descending branch of lateral circumflex femoral artery,the frequency ratio of which is up to 97.46%.Conclusion The papers on anterolateral thigh flap are written mainly by China and USA;English is the main publishing language;The articles are chiefly published by Plast Reconstr Surg and other 8 journals.The research emphases of the anterolateral thigh flap are on free skip flap,perforating flap,ultrathin skin flap,and island flap.Angiography and 3D-reconstruction are the recent techniques for the research.

AIM: To investigate the therapeutic effect and its mechanisms of musk eye drops on allergic conjunctivitis (AC) in guinea pigs.METHODS: AC was developed by intraperitoneal injection of a mixture containing Ovalbumin (OVA) and Al(OH)3 in guinea pig.Sixty guinea pigs with AC were randomly divided into 6 groups,i.e.,AC model group,three treated groups treated with musk eye drops in high,middle and low dosage,group treated with dexamethasone sodium phosphate eye drops.Together with solvent group as sixth group.After one week of administration,the changes of guinea pigs in symptoms,histamine and OVA-specific IgE concentration of ocular tissues,and morphological features of conjunctiva were observed under light microscopy.RESULTS: Compared with the vehicle group,the conjunctiva symptoms scale,histamine and OVA-specific IgE concentration of ocular tissues and morphological changes scale of conjunctiva of guinea pigs in the model group were fairly good.Compared with the model group,the ocular symptoms of guinea pigs in each of the musk eye drops treated groups showed relieved (P

Objective To evaluate the value of DSA image mosaic in arteriography in lower extremities. Methods 22 suspected cases were examined by bolus chase, and then the images were mosaiced to a full picture after processing by Paste to judge the image effects of vessels. Results This method could display the whole image by one injection of contrast media. Vary lesions were found in the 22 cases. Conclusion This method can display the lower extremities lesions directly, which is better than traditional DSA.

Objective To compare the safety and efficiency of non -ionic monomer diagnostic opaque, iodixanol (Visipaque) and that of non -ionic disomer diagnostic opaque, iohexol (Omnipaque). Methods By comparing retrospectively the DSA opacification of 60 patients from 2006 to 2007, patients′ indispositions, adverse reactions and pictorial efficiency were recorded respectively. Results In the control group, iodixanol showed less indisposition caused by injection than iohexol and there′ s no obvious difference of indisposition. Both images met diagnostic requirements. Conclusion Both iodixanol and iohexol are safe, efficient and well -tolerant diagnostic opaque. Iodixanol is better than iohexol in indisposition caused by injection.