Objective To explore the levels of interferon ?(IFN-?) and interleukin-10(IL-10) in sera and in hepatitis B virus(HBV) core antigen(HBcAg) stimulated culture supernatants of peripheral blood mononuclear cells(PBMC) from chronic HBV carriers children and their clinical significance.Methods Five milliliter peripheral blood was collected from 21 HBeAg-positive children(group A),20 HBeAg-negative children(group B) and 19 normal controls(group C),sera and PBMC was isolated,after 72 h culture of PBMC with HBcAg or not.The levels of IFN-? and IL-10 in sera and in HBcAg stimulated or not stimulated culture supernatants of PBMC were detected.Results The level of sera IFN-? had no difference between each group;the sera level of IL-10 in group A and B were significantly higher than that of group C.The level of IFN-? in HBcAg stimulated culture supernatants of PBMC from group B and C were higher than that from group A;while IL-10 levels in HBcAg stimulated culture supernatants from group A and B were higher than that from group C.Conclusions The level of IL-10 and the lower reactivity to HBcAg of PBMC play roles in the mechanisms of chronic HBV carriers children.

Objective To evaluate the value of DSA image mosaic in arteriography in lower extremities. Methods 22 suspected cases were examined by bolus chase, and then the images were mosaiced to a full picture after processing by Paste to judge the image effects of vessels. Results This method could display the whole image by one injection of contrast media. Vary lesions were found in the 22 cases. Conclusion This method can display the lower extremities lesions directly, which is better than traditional DSA.

Objective To compare the safety and efficiency of non -ionic monomer diagnostic opaque, iodixanol (Visipaque) and that of non -ionic disomer diagnostic opaque, iohexol (Omnipaque). Methods By comparing retrospectively the DSA opacification of 60 patients from 2006 to 2007, patients′ indispositions, adverse reactions and pictorial efficiency were recorded respectively. Results In the control group, iodixanol showed less indisposition caused by injection than iohexol and there′ s no obvious difference of indisposition. Both images met diagnostic requirements. Conclusion Both iodixanol and iohexol are safe, efficient and well -tolerant diagnostic opaque. Iodixanol is better than iohexol in indisposition caused by injection.

Objective To study arsenic trioxide induces apoptosis of human cholangiocarcinoma cell line QBC939. Methods MTT was applied to detect the inhibitory effect of arsenic trioxide on cholangiocarcinoma cell line QBC939. Morphological changes were observed by light microscopy and fluorescence microscopy. Rhodamine123 dyeing and analysis of DNA content and cell cycle were examined by flow cytometry. Results Cell growth was significantly inhibited by different concentration(1-16?mol/L) of arsenic trioxide. The inhibition ratio was dependent on arsenic concentration and time. The typical morphological character of ~apoptosis was observed, apoptotic peak of hypodiploid was detected and fluorescence intensity of ~Rhodamine 123 decreased when the cells were cultured with 4?mol/L As_2O_3. Conclusions As_2O_3 can induce ~apoptosis of cell line QBC939. The mechanism may be related to the depolarization of mitochondrial ~membrane potential.

Through searching literature about cytochrome P450 from PubMed, CNKI, CBM and Wanfang database in the recent 10 years, this article concluded, summarized and analyzed vivo and vitro research methods of cytochrome P450 to provide references for the study on TCM cytochrome P450 enzyme.

[ ABSTRACT] AIM:To investigate the effects of everolimus on the experimental IgA nephropathy in rats and its possible mechanisms .METHODS:The rat model of experimental IgA nephropathy was established .The rats were ran-domly divided into control group , IgA group and everolimus treatment group .After the corresponding treatments were gi-ven, urinary red blood cells , protein and N-acetyl-β-D-glucosaminidase ( NAG) were examined .Immunofluorescence stai-ning was used to analyze the level of IgA precipitation in the renal tissues .Additionally, the protein expression of myeloid differentiation factor 88 (MyD88), TLR4, NF-κB, IL-4 and IL-13 was determined by Western blot.The mRNA levels of IL-4 and IL-13 were detected by qPCR .RESULTS:Everolimus significantly inhibited the increases in the urinary levels of red blood cells, protein and NAG in experimental IgA nephropathy rats .Furthermore, IgA nephropathy-induced increases in the protein expression of MyD88, TLR4, NF-κB, IL-4 and IL-13 were attenuated after everolimus treatment .Similar re-sults were obtained in the mRNA levels of IL-4 and IL-13 by qPCR detection .CONCLUSION: Everolimus improves the impairments of renal function in experimental IgA nephropathy rats as evidenced by decreasing urinary red blood cells , pro-tein and NAG, which may be related to the inhibition of MyD 88, TLR4, NF-κB, IL-4 and IL-13 expression.

Objective To understand the status and developmental trends of the research on anterolateral thigh flap. Methods The PubMed search was downloaded and the results were imported into the EndNote database,then the distribution was analysed by age,nation,language,periodical and frequency.Results There were 236 articles on anterolateral thigh flap in PubMed from 1996 to August 2006.The amount of articles multiply increased from 2002 to August 2006,at eighty percent of all.They were published in 8 languages,in 47 journals and from 9 countries.China and the USA are the major countries of the publication,with 77 articles each,which altogether took up 65.2% of the total.Words of a high frequency are as follows: free skip flap,island skin flap,ultrathin skin flap,perforating artery,and descending branch of lateral circumflex femoral artery,the frequency ratio of which is up to 97.46%.Conclusion The papers on anterolateral thigh flap are written mainly by China and USA;English is the main publishing language;The articles are chiefly published by Plast Reconstr Surg and other 8 journals.The research emphases of the anterolateral thigh flap are on free skip flap,perforating flap,ultrathin skin flap,and island flap.Angiography and 3D-reconstruction are the recent techniques for the research.

AIM: To investigate the therapeutic effect and its mechanisms of musk eye drops on allergic conjunctivitis (AC) in guinea pigs.METHODS: AC was developed by intraperitoneal injection of a mixture containing Ovalbumin (OVA) and Al(OH)3 in guinea pig.Sixty guinea pigs with AC were randomly divided into 6 groups,i.e.,AC model group,three treated groups treated with musk eye drops in high,middle and low dosage,group treated with dexamethasone sodium phosphate eye drops.Together with solvent group as sixth group.After one week of administration,the changes of guinea pigs in symptoms,histamine and OVA-specific IgE concentration of ocular tissues,and morphological features of conjunctiva were observed under light microscopy.RESULTS: Compared with the vehicle group,the conjunctiva symptoms scale,histamine and OVA-specific IgE concentration of ocular tissues and morphological changes scale of conjunctiva of guinea pigs in the model group were fairly good.Compared with the model group,the ocular symptoms of guinea pigs in each of the musk eye drops treated groups showed relieved (P

0.05).However,the presence of TC genotype was significantly associated with an increased risk of HBV infection(P0.05).Conclusions The presence of GG genotype at the site-1082 and CC genotype at the site-819 are significantly associated with decreased risk of HBV infection,while the presence of TC genotype at the site-819 is associated with an increased risk of HBV infection.The presence of other genotypes and the frequencies of all the alleles are not associated with the risk of HBV infection.

Application of the techniques of pedicle screw fixation in t horacolumbar spine has forcefully promoted the development of spinal surgery. Th e key to the techniques is that the pedicle screw insertion must be located into the sole exact routeway of the three-dimensional-space, that is, into the inn er vertebral body through the pedicle according to the exact arrow plane angle a nd horizontal plane angle. Recently great development has been made in the resea rch on the techniques of pedicle screw insertion in thoracolumbar spine, especia lly in the supervising means during operation. The means have been developed fro m the traditional X-ray perspective or photograph apposition to the application of computer-assisted technique. This paper reviews and discusses the current d evelopment and prospects in the research on the techniques of pedicle screw inse rtion in thoracolumbar spine.