BACKGROUND:To measure the occlusal force index of patients with missing teeth before and after restoration is an effective way to evaluate the restoration effect and oral function recovery after dental restoration.OBJECTIVE:To observe the changes of occlusal force and periodontal tissue in patients undergoing CO-Cr alloy-based porcelain-fused-to-metal crown and bridge restoration.METHODS:100 male patients were randomly divided into five groups (n=20 per group), and respectively received CO-Cr alloy porcelain crown restoration of the anterior teeth (group A), CO-Cr aloy porcelain crown repair of the premolar (group B), CO-Cr aloy porcelain crown restoration of the molar (group C), CO-Cr alloy porcelain bridge restoration of the anterior teeth (group D), or CO-Cr alloy porcelain bridge restoration of the posterior teeth (group E). Changes of occlusal force and periodontal related indexes were detected before, immediately and 6 months after dental restoration.RESULTS AND CONCLUSION:(1) Occlusal force: The occlusal force in all the five groups was increased immediately and 6 months after restoration (P < 0.05). Before, immediately and 6 months after dental restoration, the occlusal force was higher in the groups C and E than the groups A, B, D (P < 0.05), as well as higher in the group B than the groups A and D (P < 0.05). (2) Periodontal detection: there were no significant differences among the five groups in the periodontal probing depth, plaque control index, clinical attachment level, and volume of gingival crevicular fluid (P > 0.05) before and after restoration. To conclude, the CO-Cr alloy-based porcelain-fused-to-metal crown and bridge restoration can significantly enhance the patient's occlusal force of the abutment teeth, exert little effect on the periodontal tissue and have good biocompatibility.

21 classII malocclusion adolescents (9 boys and 12 girls) were selected as the subjects, aged from 10.6 to 14.5 years, and the initial mean age was 12.8 years. All were treated with the modified Pendulum appli-ance. The duration for distalization of maxillary molars was from 3.2 to 5.7 months (4.3 months on average). Lat-eral cephalograms were obtained before and after distalization. Changes produced by the modified Pendulum appli-ance were analyzed with paired t tests. The mean space opening on lateral cephalograms was 7.31 mm, and maxil-lary first molar distalization accounted for 64.8% of the space,with a mean distal crown tipping of 18.64o. The rate of molar movement was 1.27 mm per month. The maxillary first molars intruded 0.69 mm,and the premolars extru-ded 1.02 mm. Lower anterior facial height increased 2.19 mm. The maxillary incisors had increased 3.39oof labial tipping and 1.13 mm of protrusion.

Objective To reconstruct three-dimensional finite element analysis all-ceramic crowm model of the first maxillary molar.Methods After the CT scanning and image processing to the first maxillary molar in vivo,a 3-D geometric model was established by the Imageware software.The mesh model was then built in Abaqus,the finite element analysis software.Result The three dimensional finite element all-ceramic crown models of the first maxillary molar was constructed on the computer,which had 60 160 nodes and 186 328 tetrahedrons elements.Conclusion The finite element model constructed has the advantages of intact structure and precise elements,can simulate the natural condition and facilitate the further biomechanical research.

Objective To observe the changes of plasma ghrelin in patients with severe sepsis and to explore the correlation of the plasma ghrelin and cytokines with sepsis.Methods Choose the healthy volunteer(n=10)as control group,and measure the plasma ghrelin(by ELISA),TNF-?,IL-1?,PCT(procalcitonin)and CRP at the 1st day,3rd day and 10th day in sepsis group(n=26).Results The plasma ghrelin level in sepsis group increased significantly compared with control group(P

BACKGROUND:Nano-calcium titanate, prepared with different modifiers, have properties of good osteogenesis, bone conductibility and biocompatibility.
OBJECTIVE:To observe the influence of nano-calcium titanate materials on the proliferation and differentiation of osteoblasts.
METHODS:Nano-calcium titanate was prepared with modifiers such as polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate. Meanwhile, nano-calcium titanate without modifier was prepared. Al the four kinds of nano-calcium titanate materials were used to prepare nano-calcium titanate extracts under the concentration of 0.1, 1.0, 10 g/L, respectively. The proliferation of osteoblasts was observed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and alkaline phosphatase activity of osteoblasts was observed. Osteoblasts cultured in normal condition served as contrast.
RESULTS AND CONCLUSION:The proliferation of osteoblasts cultured in the calcium titanate extracts prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier was obviously improved under the concentration of 1.0 and 10 g/L. Absorbance value of the cells cultured in the above-mentioned four kinds of calcium titanate extracts 1.0 and 10 g/L) for 3, 6, 9 days exceeded the contrast (P<0.05, P<0.01), as wel as the alkaline phosphatase activity (P<0.05, P<0.01). Calcium titanate prepared with polyethylene glycol, acetyl trim ethyl ammonium bromide and sodium citrate and without modifier has no cytotoxicity effect on osteoblasts, and can promote the proliferation and differentiation of osteoblasts.

Aim To isolate and characterize the human circulating fibrocytes from human peripheral blood and explore the effects of curcumin on human circulating fi-brocytes.Methods The cells were isolated and puri-fied by density gradient centrifugation,and identified by flow cytometry and immunocytochemistry .Then , CCK-8 and flow cytometry were used to study the effect of curcumin on the proliferation as well as COL I ex-pression of human circulating fibrocytes,respectively. Results After being isolated the cells expressed CD34,CD45 and COLⅠ,among which 79.7% were both CD45 and collagen I positive,typical of human circulating fibrocytes.Curcumin could exert regulatory effects on proliferation of human circulating fibrocytes. Exposure of the cells to curcumin for as short as 24 hours promoted their growth,while prolonged treatment (72 h ) significantly inhibited cell propagation and downregulated the COLⅠ levels,best manifested at a concentration as high as 20 μmol · L-1 .Conclusion The proliferation of cells and COLⅠexpressions can be effectively inhibited by curcumin with the prolonged action period and high concentrations.

Objective: To evaluate the clinical effects of full-arch cement-retained implant-supported combined crowns and screw-retained implant-supported bridge dentures in complete or half edentulous patients. Methods : A total of 25 patients with complete or partial edentulous dentures followed up for 1, 3, and 5 years in our hospital from June 2013 to June 2018 and were treated with Straumann bone horizontal implantation, cobalt-chromium stenting and cobalt-chromium porcelain restoration with cement-retained and screw-retained implant-supported fixed dental prostheses to evaluate the accumulative implant survival rate, accumulative prosthesis survival rate, mechanical complications, and biological complications in both groups. Results : There were 25 complete or half edentulous patients who received 165 Straumann implants and 28 implant-supported fixed dental prostheses in this study. There were 11 cases with 69 implants in the cement group and 17 cases with 96 implants in the screw group. The accumulative implant survival rate was 100% in the cement group and 96.9% in the screw group. The accumulative prosthesis survival rate was 100% in both groups. The cumulative peri-implant mucositis rate was 23.2% in the cement group and 29.2% in the screw group, and the peri-implantitis rate was 6.8% in the cement group and 7.3% in the screw group. There was 1 case of porcelain collapse (n=1/11) and no screw of abutment loosening in the cement group and 4 cases of porcelain collapse (n=4/17) and 1 case of screw loosening in the screw group. No fracture of abutment was observed in either group. There was no difference in bone loss between the two groups in the first year (P > 0.05), and a higher rate of bone loss was found in the screw group in the third and fifth years (P < 0.05). There was no difference in the sulcus bleeding index(mSBI) between the two groups in the first year and the third year (P > 0.05) and a higher modified mSBI value in the cement group in the fifth year (P < 0.05). Conclusion The survival rates of the implant and prosthesis for cement-retained or screw-retained implant-supported fixed dental prostheses were both high, but there were more mechanical and biological complications in the traditional cobalt-chromium alloy screw-retainer group. The removal of residual adhesives must be reasonably considered when choosing the cement retention method.

OBJECTIVE: To investigate clinical effect of individual light-curing resin tray as edge plastic material in complete denture modulo.
 METHODS: A total of 30 patients with poor condition for alveolar ridge of mandible were chosen individual tray with individual light-curing resin tray for material edge shaping or traditional individual impression tray for edge shaping cream to produce complete denture. The operability, questionnaire about denture retention, comfort, mucosal cases and chewing function in the process of shaping the edge were investigated three months later after wearing dentures.
 RESULTS: There was no significant difference in retention, comfort, mucosa and the chewing function between the two mandibular denture impression methods. However, the patients with individual light-curing resin tray as edge shaping material felt better in the process than that in the patients with die-cream as the edge shaping material (P<0.05). Furthermore, the manipulation with individual light-curing resin tray as edge shaping material is easy for doctor.
 CONCLUSION Although the clinical effect of Individual light-curing resin tray material as the edge shaping material is equal to that of impression cream, it saves time and human resource. Moreover, it is more acceptable for the patients and thus it can be spread in clinics.
Acrylic Resins ; Dental Impression Materials ; Dental Impression Technique ; Denture, Complete ; Humans ; Light-Curing of Dental Adhesives ; instrumentation

BACKGROUND: Regenerative medicine by using stem cells from dental pulp is promising for treating patients with critical limb ischemic (CLI). Here, we investigated the difference in the angiogenetic ability of stem cells from human exfoliated deciduous teeth (SHED) and human dental pulp stem cells (DPSC). METHODS: SHED and DPSC were harvested from dental pulp and analyzed in flow- cytometry for detecting the expression of surface markers. Levels of angiogenetic marker were examined by RT-PCR and Western-blot. Eighteen immunodeficient mice of critical limb ischemic model were divided into three groups: SHED, DPSC and saline, which was administered with SHED, DPSC or saline intramuscularly. Histological examination was performed to detect the regenerative results. RESULTS: A highly expression of CD146 was detected in SHED. Moreover, cells with negative expression of both CD146 and CD31 in SHED were more in comparison with those in DPSC. Expression of angiogenesis factors including CXCL12, CXCR4, Hif-1a, CD31, VEGF and bFGF were significant higher in SHED than DPSC by the RT-PCR and Western-Blot results. SHED induced more CD31 expression and less fibrous tissue formation in the critical limb ischemic model as compare with DPSC and saline. CONCLUSION Both SHED and DPSC possessed the ability of repairing CLI. With expressing more proangiogenesis factors, SHED may have the advantage of repairing CLI.

Objective:To discuss how to avoid the occurrence of adverse events and provides basis for improving the extracorporeal membrane oxygenation (ECMO) transport safety management to formulate the corresponding preventive measures through analyzing the causes and characteristics of adverse events in transport of ECMO.Methods:By using a self-designed ECMO transport observation table to collect data, with a retrospective study of adverse events in patients with ECMO transport in ECMO center of Beijing Chaoyang Hospital from January 2013 to June 2017, carrying out classification and analysis according to the causes of adverse events and the potential risks of the patients, thus put forward the feasible preventive measures.Results:There were 53 cases of ECMO transport in study period, with 18 cases (33.96%) of adverse events, among which the incidence of adverse events in inner-hospital transport was 34.21% (13/38) and that in inter-hospital transport was 33.33% (5/15). There was no patient died in ECMO transport. In the adverse events of ECMO transport, the main causes were related to transport staff, transport equipment and patient, which accounting for 1/3 of each. Among them, the most prominent was 4 cases (22.22%) of equipment lacking and 3 cases of battery and power supply (16.67%). In classification according to the risk degree of patients, 6 cases (33.33%) of third grade risk were found.Conclusions:It is safe and feasible to carry out ECMO transport in inner-hospital transport and inter-hospital transport based on ECMO transport team and transport process of this hospital. However the unexpected events of high risk or crisis of life is inevitable in ECMO transport. Through standardized training for ECMO team, with full assessment before transport, by the use of ECMO checklist and strict implementation of various transport processes and specifications, the incidence of adverse events in ECMO transport may be reduced.