Objective To study the relationship of Hp infection and gastric juice vitamin C concentration to gastric mucosa lesions,as well as its mechanisms of pathogenesis and carcinogenesis.Methods Vitamin C concentration in gastric juice was measured by using reduced ferric method.Hp was determined by fast urease test,pathologic Giemsa stain and bacterial culture.Hp eradication treatment was performed on part of Hp positive patients,and the vitamin C concentration in gastric juice before and after Hp eradication was compared. Results Gastric juice vitamin C concentrations in each Hp positive test groups were:(18.3±4.5)mg/L(chronic superficial gastritis group),(9.1±3.3)mg/L(atrophic gastritis with intestinal metaplasia group),(8.2±2.7)mg/L(dysplasia group),and (5.2±1.6)mg/L(gastric cancer group) respectively,which were significantly lower than that in control group [(35.2±7.2)mg/L],P＜0.05.After eradication of Hp,the vitamin C concentrations in gastric juice returned to normal,whereas it had no significant difference in those with failure of Hp eradication.Conclusions Hp infection may cause a significant lowering of gastric juice vitamin C concentration,which may be one of the mechanisms of its pathogenesis and carcinogenesis.Eradication of Hp can return the vitamin C concentration in gastric juice to normal,which may contribute to guard against the occurrence of gastric cancer.

Postoperative pain is an important factor which affects patients'postoperative rehabilitation training.With the application of ultrasound guided nerve block and multimodal analgesia,the quality of postoperative pain management has improved obviously among patients after foot and ankle surgery.The study has reviewed the related research.

With the progresses in medical research, reconstruction of body's deformation because of tumor or injury has been drawn more and more attentions. As autograft was limited by in short supply and dissatisfied performance, material scientists and medical researchers worked hard together and laid emphasis on manmade materials. Early studies were focused on absorbable materials. Since there was great discrepancy between absorption time and tissue repairing time, the studies on absorbable materials were blocked. From 1960's, more and more studies focused on bioceramics because of its excellent biocompatibility and mechanical properties. But the brittleness of bioceramics limited it for clinical use. In recent time, the strengthened ceramics and nano-bioceramics have been paid more attention and made great success. Scientists foretell that nano-technology would be the strategy of changing the brittleness of ceramics. The characters of nano-compound bioceramics were fit for the mechanical properties demand of man's hard tissue more. That infused new vigor into the research of bioceramics.

Objectire To investigate female urogenital fistula on the cause,diagnosis and treatment.Methods Thirty nine cases of patients were diagnosed ashaving genitourinary fistula by cystoscopy,intravenous urography,and blue methvlenelest.In 51%of cases were uretero-vaginal fistulas(20/39),one of them were trated byrenal ostomy.17 cases by early emergency ureteroneocystomy,2 cases by ureteralcatheter.In two complex patients with vesico.uretero-vaginal fistula(2/39),the repairwas associated with badder approach vesicovaginal fistula and early emergencyureteroneocystomy.In 44%of cases with vesicovaginal fistula(17/39)were trated bybladder approach after 3～27 months.Results 36 cases of patients were cured after surgery in one time,2 cases of ureteral intubation,1 case of nephrostomy and nephrectomy later with renal infection,1 case of uroclepsia complication.Conclusion Surgical repair of genitourinary fistula will have high rates of success if performed in proper time and appropriate approaches on the basis of patientinf eirmation and urinary fistula pathological type by experienced hands.

Objective To detect the normal value of the fetal middle cerebral artery peak systolic velocity (MCA-PSV) with Doppler ultrasonography, and to investigate the association between MCA-PSV and fetal anemia. Methods Doppler ultrasonography was performed in 535 normal pregnant women from the 14th to the 40th week of gestation. MCA-PSV was measured, and the standard value of the normal fetal MCA-PSV was established. Fetuses with abnormal high MCA-PSV were assessed with a standard of MCA-PSV＞mean vale of gestational weeks+2 standard deviation or 1.5 multiples of mean value of gestational weeks. Results The value of normal fetal MCA-PSV correlated well with the gestational weeks (linear regression equation was: MCA-PSV＝-9.955+1.561×weeks; r＝0.795, P<0.0001). Using 1.5 multiples of the mean of different gestational weeks to diagnose the moderate and severe fetal anemia, 7 fetuses were screened out of 209 of obstetric ultrasonography; 4 of them had MCA-PSV＞1.5 multiples of mean value of gestational weeks and were proved with induced labor. Conclusion The value of 1.5 multiples of mean value of gestational weeks of fetal MCA-PSV in prenatal ultrasonography might be an important clue for the diagnosis of fetal anemia.

Objective　To analyse the CT appearance of ependymomas and to improve the accuracy of diagnosis. Methods　To review the CT appearance of 15 cases of ependymoma that confirmed by surgery and pathology. Results　12 cases were superior, 8 cases were brain parenchyma among of them; 3 cases were subtentorial, among them the fourth ventricle 2 cases. The age of 6 cases vary from 20 to 35 years. Ependymomas superior to the tentorium in the brain parenchyma were the most common, and in malignant parts there were cystoid changes and edema around the tumor. In the other parts were largely solid neoplasm. On unenhanced CT, the solid parts were indense and caicificable and were easy to dicern on enhanced CT . Conclusion　The CT appearances of ependymoma were specific, we can dram an accurate conclusion according where the tumor taking place, the age etc.

Objective To explore the anti-inflammatory and anti-angiogenic effect of miR-15a in diabetic retinopathy(DR).Methods High glucose model(HG)was established by treatment with 25 mmol/L glucose in human retinal pigment epithelial cell line HARPE-19,and HARPE-19 cells treated with 5 mmol/L glucose served as control(LG).HG group and LG group were divided into 4 subgroups:miR-15a mimic group,NC-mimic group,miR-15a inhibitor group and NC-inhibitor group.Cell proliferation ability was detected by MTT proliferation assay.Cell migration ability was detected by cell scratch assay.The relationship between miR-15a and ASM was identified by 3′UTR luciferase reporter assay.miR-15a,ASM,VEGF,IL-1β,IL-6,TNF-α mRNA expression was detected by RT-PCR method.Results In NC-mimic cells and NC-inhibitor cells,the expression of miR-15a in HG group was significantly lower than that in LG group(all P<0.05).The expression level of ASM mRNA in miR-15a mimic group was significantly lower than that in NC-mimic group (P<0.05),the expression level of ASM mRNA in miR-15a inhibitor group was significantly higher than that in NC-inhibitor group (P<0.05).The cell scratch width in miR-15a mimic group was significantly higher than that in NC-mimic group(P<0.05).The cell scratch width in miR-15a inhibitor group was significantly lower than that in NC-inhibitor group(P<0.05).The absorbance value in miR-15a mimic group was significantly higher than that in NC-mimic group(P<0.05),the absorbance value in miR-15a inhibitor group was significantly lower than that in NC-inhibitor group(P<0.05).In NC-mimic cells and NC-inhibitor cells,the mRNA expression levels of VEGF,IL-1β,IL-6 and TNF-α in HG group was significantly higher than those in LG group(all P<0.05).Conclusion miR-15a downexpression induces inflammatory reaction and angiogenesis in retinal cells.miR-15a is involved in the pathogenesis of DR by dual regulation of inflammation and angiogenesis.

Objective To know rural drinking water safety and the affecting factors in Guangxi Zhuang Autonomous Region.Methods The survey was conducted through a combination of census and spot-checks during January to May 2005,taking villages as the survey units,the basic situation of water supply,coverage of tap water,running of water supply facilities,and the distribution and types of water sources,pollution of water sources,water sources and beneficiary population were investigated.The water samples were collected and tested with related standard methods on July and November,2005.Results The total rural population in Guangxi Zhuang Autonomous Region was 41.424 0 million,13.858 1 million people were served with central water supply,accounted for 33.45%.There were 14 260 township and village level central water supply systems.Of these systems,only 1 157 had water purification facilities,accounted for 8.11%.A total of 475 water samples were tested,the eligible was 25.68%.The eligible rates were 23.71% and 27.57% in rainy and dry seasons,respectively.The bacteriological indexes were the main noneligible indexes,as for total coliform,the eligible rate was less than 40%.The rural population consuming unsafe drinking water was 17.768 6 million,accounted for 42.9%.Conclusion The drinking water safety in rural areas in Guangxi Zhuang Autonomous Region is to be solved urgently.Special focus should be paid to the construction and management of rural drinking water safety projects.

The clinical data of one case of laryngeal neuroendocrine carcinoma in our hospital were respectively analyzed. The patient was a 61-year-old male, and complained of progressive pain of right ear for 7-year, it was misdiagnosed as glossopharyngeal neuralgia and larynx hemangioma. This patient was achieved total laryngectomy and bilateral cervical lymph nodes dissection. As of 12 months after surgery, the patient remains under follow-up observation, and no findings indicating obvious recurrence or metastasis has been observed. This disease is a rare and highly malignant tumor that is lack of specific feature in clinical performances. Recognition at larynx is essential so that proper patient management can be initiated.
Carcinoma, Neuroendocrine ; diagnosis ; therapy ; Humans ; Laryngeal Neoplasms ; diagnosis ; therapy ; Male ; Middle Aged

Objective To explore the risk factors of asthma in children with bronchiclitics.Methods One hundred and two cases hospita-lized patients with bronchiolitis were analyzed and followed-up for 2-7 years,items of follow-up included sex,morbidity age,food feeding,severity of disease,atopy physique,family history of atopy/asthma,environment exposure,passive smoking,and so on.The factors were carried on the Logistic regression analysis to determine the influence on later years to having recurrent asthma.Results Forty-seven cases had transferred asthma,which was remarkably higher than the domestic urban survey data(0.12%-3.34%).The multi-factors Logistic regression analysis demonstrated that environment(OR 6.099,95%CI 1.824～20.396),passive smoking(OR 49.222,95%CI 8.219-294.774),atopy physique (OR 45.548,95%CI 6.799-305.117)and family history of atopy/asthma(OR 47.921,95%CI 8.626-266.226)were the risk facter of asthma( Pa