ObjectiveTo study the effect of salmon calcitonin in treating knee osteoarthritis.MethodsFortyeight cases of knee osteoarthritis were randomly divided into four groups,twelve cases each group.A group:acetaminophen+glucosamine hydrochloride,B group:meloxicam+glucosamine hydrochloride,C group:salmon calcitonin + glucosamine hydrochloride,D group:salmon calcitonin.Acetaminophen and meloxicam can stop as pain symptoms disappeared.Other drugs were treated for 12 weeks.A follow up was carried out to compare visual analogue score and 12-item short form health survey.ResultsTwelve weeks later,A,B,C,D four groups' visual analogue score decreased from before treatment 5.6±1.2,5.5±1.2,5.4±1.0,5.1±l.2 to 1.9±0.8,1.2±0.7,0.8±0.6,0.9±0.7,12-item short form health survey score from before treatment - 23.4±3.1,- 23.8±3.0,-24.8 ±3.8,-22.4±2.8 to -15.1±4.7、- 14.5±4.7,-9.3±6.2,-10.2±5.3.There was significant difference before and after treatment in every group(P＜ 0.05).The visual analogue scores of B,C and D group outweigh A group's (P＜ 0.05).A and B groups'scores of 12-item short form health survey were lower to C and D groups' ( P ＜ 0.05 ).ConclusionsSalmon calcitonin can relieve pain in treating knee osteoarthritis.

Objective: To investigate frequencies of microsatellite instability (MSI) and loss of heterozygosity LOH) in renal cell carcinoma (RCC), and to discuss the relationship of clinicopathological characteristics of RCC with MSI and LOH. Methods: Twelve microsatellite markers located at chromosomes 3p, 9p and 14q were selected to investigate microsatellite alterations (MSI and LOH) in 31 RCC specimens and their paired metastasis specimens by polymerase chain reaction-polyacrylamide gel electrophoresis-ethylene dibromide (PCR-PAGE-EB) staining and sequencing. Results: The frequency of MSI could reached 61.3% and that of LOH could reach 54.8%. The highest frequency of MSI was at locus of D9S168 (32.3%); the highest frequency of LOH was at locus of D3S1289 (21.4%). No correlation was found between MSI or LOH and the patients' age, sex, pathology type and metastastis, except that MSI was correlated with TNM stage of RCC (P<0.05). Conclusion: MSI and LOH of 12 microsatellite markers, except for D3S1566, might be risk factors for RCC. D9S168 and D3S1289 are two sensitive loci in RCC, and they might be close to RCC associated oncogenes or anti-oncogenes, which may influence the development and progression of RCC.

Objective To explore the application of magnetic resonance angiography and virtual reality technique in preoperative planning for sphenoid ridge meningioma. Methods Ten patients with sphenoid ridge meningiomas were examined by brain CT, MRI and MRA before operation. Then the image data were transferred to the Dextroscope system. The images were reconstructed using the Radiodexter software to display the three dimensional image. The tumor and its adjacent anatomic structures could be viewed and the system tools allowed for simulation of the operation, so the full information of the tumor, basilar texture, and nerves and vessels could be obtained. A pre-operative planning was done and was compared with intra- operation situation. Results The virtual reality images of the 10 cases were successfully conducted, and all the procedures were performed according to the pre-operative planning, with a consistent rate of 100%. Radical resection was achieved and all the 10 patients and there were no sequelea. Conclusion Magnetic resonance angiography and the virtual reality system can provide the information of tumor and its adjacent structures, so it can Hp the neurosurgeons no planning the details for operation, contributing to operation safety and tumor resection.

Objective To explore the value of computed tomographic DacryocystographyCT-DCGand computed tomographic three-dimensional3Dreconstruction with lacrimal passages in asymptomatic lacrimal flow patients with nasal structural abnomalities. Method: In 2006-2010, 18 nasal structural abnomalities cases with asymptomatic lacrimal flow and nasal obstruction were enrolled, the lacrimal flow caused by ocular region was obviated. Of all the 18 hypertrophic inferior turbinate cases, there were 12 cases accompanied with nasal septum deviation. The radiofrequency ablation of inferior turbinate and nasal septum deviation surgery under nasaI endoscope were takenThe CT-DCG and 3D reconstruction examines were observed preoperative and postoperative. All the patients were followed-up for 12 months. Result: 18 nasal structural abnomalities were corrected. The nasal obstruction were recoveried and improved in 1477.8%and 422.2% cases respectively. The symptom of epiphora had disappeared and improved in 1372.2% and 5 cases27.8%. Partial obstruction of the internal ostium was found preoperative and resolved postoperative with CT-DCG and 3D reconstruction. Conclusion: CT-DCG and 3D reconstruction can evaluate the asymptomatic alterationsof the normal lacrimal outflow in cases with nasal structural abnomalities, suggesting that they have great value in clinical applications.

Amyloid beta-Peptides ; analysis ; Animals ; Frontal Lobe ; drug effects ; physiology ; Hippocampus ; drug effects ; physiology ; Learning ; drug effects ; Memory ; drug effects ; Rats ; Rats, Wistar ; Sodium Azide ; pharmacology ; Space Perception

With the increasingly aging population,osteoporosis morbidity had been gradually increased and the incidence of the hips and vertebral fractures showed a trend of rapid growth,which brought great pain and economic burden to patients and society.There were a variety of anti-osteoporosis drugs clinically,which interfere with the development of osteoporosis via multi-approaches to reduce the clinical symptoms and improve their quality of life.In this paper,the author aimed to make a review of the research progress of anti-osteoporosis drug clinical application.

AimTo evaluate the effects of different doses of valsartan on blood pressure and left ventricular hypertrophy in spontaneously hypertensive rats (SHR) . MethodsEighteen SHR(fourteenweek-old, male) were divided into three groups (six rats in each group ): SHR control group in which the rats were fed with normal saline; low dose valsartan group in which the rats were fed with valsartan 8 mg· kg-1 · d-1 and high dose valsartan group in which the rats were fed with valsartan 24 mg · kg -1 · d-1, all for 8 weeks. The rats in the WKY control group(n ＝ 6) were fed with normal saline for 8 weeks. Results SBP, LVM/ BW and TDM of SHR were remarkably lower than those of the control after drug intervention, and the effect on SBP, LVM/ BW and TDM was most remarkable in the high dose valsartan group. ConclusionDifferent doses of valsartan can decrease SBP of SHR and inhibit the progression of ventricular hypertrophy.

OBJECTIVETo evaluate the clinical effects of the massage method of micro-regulating with vertical cross pressing lying on one side in treating cervicogenic headache (CEH).

METHODSTotal 136 patients with CEH were collected in the study from August 2012 to April 2014. They were divided randomly into two groups according to random digits table. Sixty-nine patients accepted the treatment of micro-regulating with vertical cross pressing lying on one side (pressing micro-regulating group), including 29 males and 40 females with an average age of (50.55 ± 11.38) years old; 67 patients received the treatment of traditional massage (traditional massage group), including 28 males and 39 females with an average age of (51.20 ± 11.90) years old. Clinical effect was observed according to the standard of curative effect of State Administration of Traditional Chinese Medicine; the function of cervical vertebra and all body status were evaluated according to NDI score. VAS score, frequency and time of headache were recorded and compared before and after treatment.

RESULTSNo adverse reactions were found after treatment, all patients were followed up from 1 to 6 months with an average of 3.1 months. In pressing micro-regulating group, 25 cases got fully recover, 26 excellence, 14 effectiveness and 4 inefficiency; and in traditional massage group, the results were 12,21,22, 12;clinical effect of pressing micro-regulating group was better than that of traditional massage group (P<0.01). NDI score in pressing micro-regulating group decreased from preoperative 13.48 ± 4.83 to postoperative 6.23 ± 3.76; in traditional massage group also decreased from preoperative 13.82 ± 5.78 to postoperative 8.25 ± 4.75; the improvement of the pressing micro-regulating group was obviously better than that of traditional massage group (P < 0.01). VAS score in pressing micro-regulating group decreased from preoperative 4.75 ± 0.97 to postoperative 1.88 ± 1.78; and in traditional massage group decreased from pre-operative 4.78 ± 0.98 to postoperative 2.84 ± 1.94; pressing micro-regulating group was more notable than that of traditional massage group (P < 0.01). The frequency per week,the pain time in pressing micro-regulating group decreased from preoperative (5.38 ± 1.96) times and (6.87 ± 3.67) hours to postoperative (1.71 ± 2.04) times and (0.97 ± 1.74) hours,respectively,in traditional massage group the above parameters decreased from preoperative (5.22 ± 1.81) times and (6.90 ± 3.79) hours to postoperative (2.81 ± 2.42) times and (1.83 ± 2.21) hours;pressing micr-regulating group was more notable than that of traditional massage group (P < 0.01 or P < 0.05).

CONCLUSIONUsing the tuina method of micro-regulating with vertical cross pressing lying on one side to treat CEH can improve function of cervical vertebra and all body status, lessen the intensity, frequency, duration time of pain, and had advantage of higher security, simple operation, and evident effect.

Adult ; Aged ; Female ; Humans ; Male ; Massage ; methods ; Middle Aged ; Physical Therapy Modalities ; Post-Traumatic Headache ; therapy

Objective To study the chemical constituents of Acanthus ilicifolius. Methods Compounds were isolated and purified on silica gel,SephadexLH -20 and HPLC,and their chemical structures were elucidated by spectral evidence,physical and chemical analyses. Results and Conclusion Nine compounds,named as stigmasta-4-en-3-one(1),stigmasta-4,22-diene-3-one(2),campest-4-en-3-one(3),tigmasta-4-en-3,6-dione(4),Stigmasta-4,22-diene-3,6-dione(5),Stigmasta-4,22-diene-6?-ol-3-one(6),6?-hydroxyl-stigmasta-4-en-3-one(7),3?-hydroxyl-stigmasta-5,22-diene-7-one(8),3?-hydroxyl-stigmasta-5-en-7-one(9),respectively,were obtained from the petroleum ether soluble portion in the methanol extracts of Acanthus ilicifolius. Among them,compounds 4~8 were reported from this plant for the first time,compounds 2,4 and 9 showed moderate activity against selected tumor cell lines.

Objective: To observe the effect of muscle regions of meridians warm needling method plus pricking Jing-Well points for blood-letting in improving nail fold microcirculation in the patients with shoulder-hand syndrome (SHS) after stroke, and the effects on hemorrheology, calcitonin gene-related peptide (CGRP) and serum substance P (SP). Methods: A total of 72 patients were randomized into an observation group and a control group by the random number table method, with 36 cases in each group. The control group was treated with physical rehabilitation training, and the observation group was treated with additional muscle regions of meridians warm needling method plus pricking Jing-Well points for blood-letting treatment. The treatment course lasted for 4 weeks. After treatment, the clinical efficacy of the two groups was compared. The changes in shoulder-hand syndrome scale (SHSS), simplified Fugl-Meyer assessment-upper extremity (FMA-UE), visual analog scale (VAS), activities of daily living (ADL), traditional Chinese medicine (TCM) syndrome score, nail fold microcirculation hemorheology indictors [whole blood viscosity (high-shear, low-shear), hematocrit, erythrocyte sedimentation rate (ESR)], CGRP and SP levels were observed. Results: The total effective rate in the observation group was 86.1％, higher than 63.9％ in the control group (P<0.05). The overall curative effect in the observation group was better than that in the control group (P<0.05). After treatment, the scores of pain sensation, edema, external turn and rotation of the arm in SHSS, and the total score were significantly decreased in both groups (all P<0.05), and each score in the observation group was lower than that in the control group (all P<0.05). After treatment, the scores of VAS and TCM syndrome in both groups decreased significantly (all P<0.05), and the scores of FMA-UE and ADL increased significantly (all P<0.05). The scores of VAS and TCM syndrome in the observation group were lower than those in the control group (both P<0.05), and the scores of FMA-UE and ADL were higher than those in the control group (both P<0.05). After treatment, the whole blood viscosity (high-shear and low-shear) and hematocrit in both groups decreased obviously (all P<0.05), and ESR increased obviously (both P<0.05), and the whole blood viscosity (high-shear and low-shear) and hematocrit in the observation group were lower than those in the control group (all P<0.05), and ESR was higher than that in the control group (P<0.05). After treatment, the peritubular state, loop shape, blood flow and total score of nail fold microcirculation in both groups decreased significantly (all P<0.05), and each score in the observation group was lower than that in the control group (all P<0.05). After treatment, SP in both groups decreased obviously (both P<0.05), CGRP increased obviously (both P<0.05), and SP in the observation group was lower than that in the control group (P<0.05), CGRP was higher than that in the control group (P<0.05). Conclusion: Compared with conventional physical rehabilitation training, muscle regions of meridians warm needling method plus pricking Jing-Well points for blood-letting treatment can significantly reduce the clinical symptoms of SHS, promote the recovery of physical functions, improve the nail fold microcirculation and hemorrheology indictors, and regulate the serum cytokine levels such as CGRP and SP.