Objective To study the effect of using improved loop electrosurgical excision procedure for diagnosis and treatment of cervical diseases. Methods The clinical data of 70 cases of cervical intraepithelial neoplasia or carcinoma in situ from January 2006 to Decemder 2008 in our hospital was collected.The operation time, the mean volume of blooding, days of blooding, relationship between the mean blood and areas of pyramid area of pyramid was compared between study group and control group. Improved loop electrosurgical excision procedure was executed on 21 cases of study group ( CIN Ⅰ 4 cases, CIN Ⅱ 10 cases,CIN Ⅲ4 cases, CIS3 cases ) and loop electrosurgical excision procedure was used on 49 cases in control group (CIN Ⅰ7 cases,CIN Ⅱ21 cases, CIN Ⅲ 16 cases, CIS5 cases). Results Blood loss [(16.57 ±17. 47 ) ml], operative time [( 9.07 ± 3.34 ) min] and postoperative bleeding time [(9.10 ± 2.23) d] in study group were lower than that in control group [( 39.80 ± 37.43 ) ml, ( 12. 19 ± 5.34 ) min, ( 10. 49 ±2. 76)d], and the differences were statistically significant ( P < 0. 05 ). Cervical cone volume [( 14. 70 ±13. 35 ) mm3] and blood loss [(32. 83 ± 14. 32 ) ml] were positively correlated ( r = 0. 430, P < 0. 05 ). No significant difference between prognosis of two groups was found( P >0. 05 ). Conclusion There was positive relationship between the averge blood loss in surgery and areas of pyramid excision. It'd be better to stitch the ramus descendents of uterine artery at three and nine o'clock of the cervix uteri, when the patients were expected to have large pyramid areas and abundant blood flow who may bleed more than normal. which can reduce the blood loss after surgery and reduce the time of surgery.

According to topographic anatomy, pathogenesis and by retrieving, summarizing and analyzing literature regarding needle-knife and needles with knife-edge for carotid cardiac syndrome, it is found out that clinical misdiagnosis rate of carotid cardiac syndrome is considerably high. Needle-knife and needles with knife-edge could significantly improve the clinical symptoms of carotid cardiac syndrome, showing characteristic and advantage in treatment, but it is deficient in technique standard and efficacy criteria that should be united and authoritative. Researches regarding pathogenesis of carotid cardiac syndrome are not systematic. Clinical observation regarding long-term efficacy and relapse of needle-knife and needles with knife-edge treatment is rare. It is believed that the awareness on carotid cardiac syndrome should be increased to reduce misdiagnosis; scientific and standardized technique standard and efficacy criteria should be established; systematic and comprehensive researches regarding mechanism of needle-knife and needles with knife-edge for carotid cardiac syndrome should be launched; besides, clinical discussion regarding its long-term efficacy should start to provide a better clinical guideline.
Acupuncture Therapy ; instrumentation ; methods ; Heart Diseases ; therapy ; Humans ; Needles ; Treatment Outcome

Objective To evaluate the effectiveness and safety of percutaneous subclavian venous catheterization through the point of insertion medial to the middle of clavicle in infants.Methods One hundred and fifty infants(88 male,62 female)aged 1-12 months scheduled for major operation were enrolled in this study. Subclavian venous catheterization was performed via supraclavicular approach.The needle was inserted at a point midway between the middle of clavicle and the inner third of clavicle(group Ⅰ,n=50)or at the point where the posterior border of sterno-cleidomastoid muscle(SCM)meets the upper border of clavicle(Yoffa method group Ⅱ, n=50)or at the posterior border of SCM,0.5-1.0 cm above the upper border of clavicle(modified James method,group Ⅲ,n=50).The overall success rate,the success rate at first attempt,the failure rate,the depth of insertion and complications were recorded.Results The 3 groups were comparable with respect to sex ratio,age and body weight.There were no significant differences in overall success rate and failure rate among the 3 groups. The success rate at first attempt in group Ⅰ(86%)was significantly higher than that in group Ⅱ(74%)and group Ⅲ(80%)(P＜0.05).The depth of insertion was significantly greater in group Ⅰ[(2.21+0.28)cm]than in group Ⅱ[(1.79?0.33)cm]and group Ⅲ[(1.84?0.38)cm].No pneumothorax and in advertent arterial puncture and nerve injury occurred in the 3 groups.Conclusion Supraclaricular subclavian venous catheterization through the insertion point medial to the middle of clavicle is better than the conventional methods(Yoffa,James) in terms of success rate at first attempt in infants.

Objective To evaluate the feasibility of catheterization of subclavian vein via supra-clavicular puncture in children less than six years of age, in comparison with subclavian vein catheterization via infraclavicular puncture and cannulation of internal jugular vein (IJV) from a high medial approach. Methods One hundred and eighty children (104 male, 76 female) aged 15 d-6 yr, undergoing major surgery were randomly divided into 3 equal groups of 60 patients: group I subclavian vein catheterization via supra-clavicular puncture; group Ⅱ subclavian vein catheterization via infra-clavicular puncture and group Ⅲ IJV catheterization from a high medial approach. (1) The overall success rate of puncture, (2) the success rate of puncture at first attempt, (3) the success rate of cannulation, (4) the duration from beginning of puncture to aspiration of blood, (5) the duration from the beginning of puncture to successful cannulation and (6) complication were recorded. Chest X-ray was taken in 30 patients in group I for confirmation of the position of catheter in subclavian vein. Results (1) The overall success rate of puncture was 100% and the success rate of puncture at first attempt was 86.67% in group I significantly higher than those in group Ⅱ (91.96% and 51.67%) and group Ⅲ (93.33% and 58.33%) (P

Objective To investigate the coordinate repression of angiostatin(AS)and Fas gene on human colon carcinoma LOVO.Methods Plasmid pcDNA3-AS and pcDNA3-Fas were constructed,and AS,Fas,AS and Fas gene were transfected to human colon carcinoma LOVO cells by liposome method.The expressions of target protein were detected by Western blot.The effects of transfection of AS and Fas gene on the growth of human colon carcinoma cells were detected by MTr methods.AS,Fas,AS and Fas gene were transfected to the human colon carcinoma subcutaneously implanted in nude mice by direct injection into the tumor.The tumor sizes were detected after 14 days of the first gene transfection.Results The effect of gene transfeetion in LOVO cell after twelve hours,24 h,48 h and 72 h were observed and compared with control group,co-transfection of Fas and AS gene group and Fas group significantly inhibited the growth of human colon carcinoma LOVO line cells(P＜0.01).Fourteen days after plasmid transfection,the tumor volume in group Fas and group co-transfection were signifieandy smaller than that of control,AS and Fas group(P＜0.05).The tumor volume in AS group and Fas group were significantly smaller than that of control(P＜0.05).Conclusion Angiostatin and Fas gene have coordinate repression effect on human colon carcinoma in vivo.

Objective To investigate the expression and distribution of leptin in pre-implantation embryo,and its effect on mouse embryo development in vitro. Methods Adult NIH female mice were superovulated and then mated with fertile males of the same strain. On the morning of 1st to 4th d of pregnancy,the oviduct and horn of uterus were dissected out and flushed with DMEM/F12 medium adequately. The embryos collected were subjected to the following procedures: ①Total RNA was isolated from embryos of each stage respectively and mRNA expression of leptin was detected by RT-PCR. ②Immunofluorescence staining was performed on blastocysts to analyze the expression and the distribution of leptin protein under laser confocal microscope. ③Eight-cell embryos were cultured in medium containing leptin antibody at various concentrations to observe the formation and hatching of blastocysts. Results ①Leptin mRNA expression was only detected in blastocyst,and no expression was detected in embryos in other stages. Leptin protein was detected in cytoplasma,membrane of trophoblastic cell and inner cell mass,however no expression was detected in cell nucleus. ②Leptin polyclonal antibody significantly inhibited formation of blastocysts (1∶400,P

Objective To study the DNA damage effect of the organic extracts from Chlorinated domestic sewage coming from three different places in vivo and in vitro. Methods The domestic sewage samples were collected from different places:university A, university B and a hotel. The DNA damage effect was tested in the bacteria, cells and BALB/C mouse by single cell gel electrophoresis (comet assay), the micronucleus test, Ames test. Results Comet assay indicators:all three samples, before chlorinated, at the dosages of 1.0, 5.0 mg/ml, after chlorinated, at the dosages of 0.04, 0.2, 1.0, 5.0 mg/ml for university A, 0.2, 1.0, 5.0 mg/ml for a hotel, 1.0, 5.0 mg/ml for university B, were significantly higher than those in the negative group(P

[Objective]To evaluate the clinical value in treatment of subtrochanteric fracture of the femur by dynamic condylar screw(DCS).[Method]This study retrospectively reviewed 29 patients who suffered from subtrochanteric fracture of the femur.There were 18 males,11 females,with an average age of 52.2 years.Seven cases were caused by traffic accident,8 cases caused by falling from hight,14 cases caused by pedestrian injury.According to Seinsheimer classification,there were 5 cases of typeⅡ(2 of typeⅡB,3 of typeⅡC),12 cases of typeⅢ(7 of type ⅢA,5 of type ⅢB),5 cases of type Ⅳ,7 cases of type Ⅴ.All patients accepted the treatment with traction reduction and dynamic condylar screw fixation and were fellowed up for 9～24 months(average 16 months).Outcoms were assessed by Sanders traumatic hip rating scale and postoperative complications were analyzed.[Result]All patients obtained bone union in average 3.5 months(3～5 months),Only one case who had obtained bone union suffered subtrochanteric refracture 16 months after operation because of plate breakage.All patients were encouraged to early exercise and walk by nonweight-bearing.There was no postoperative infection,deep venous thrombosis,internal fixation loosening,limb shortening and varus deformity.According to sanders traumatic hip rating scale,18 cases were excellent,10 cases were good,1case was fair.The excellent and good rate was 96.5%.[Conclusion]In treatment of subtrochanteric fracture of the femur,DCS not only maintain satisfying reduction with reliable fixation,but also prevent femoral shortening,rotation and varus deformity,thus get good curative effect,fast recovery,satisfying function and so on.So it is one of the best choices to heal subtrochanteric fracture.

HIV-1 integrase (IN) is an essential enzyme for retroviral replication. There is no analogue for this enzyme in human cells so that inhibition of IN will not bring strong effect on human body. Thus, HIV-1 IN has become a rational target for therapy of AIDS. This review provides a comprehensive report of alpha, gamma-diketo IN inhibitors discovered in recent years. Compilation of such data will prove to be beneficial in developing QSAR, pharmacophore hypothesis generation and validation, virtual screening and synthesis of compounds with higher activity.

[Summary] Berberine is akind of alkaloids extracted from Chinese herb medicine in cludingphellod endron ,coptis and radix berberidis .In recent years ,the pharmacological effects of berberinewas investigated extensively including anti-infection ,anti-arrhythmia ,protection of ischemic myocardium ,anti-hypertension , anti-tumor ,anti-HIV .And there are increasing reports about its hypoglycemic effect ,but its mechanism remains unclear .Here we summarize the possible mechanism of hypoglycemic effect and clinical efficacy of berberine .