Objective:To investigate the expression rule of Cyclin-E in endometrium during the early pregnancy and its role in blastocyst implantation in mice.Methods:RT-PCR and immunohistochemistry techniques were applied to detect Cyclin-E gene and protein expression in endometrium from pregnant mice on days 1～7.In addition Anti-cyclinE antibody was injected into horns of uterus of day 3 pregnant mice to observe the numbers of implanted blastocysts.Results:Expression of Cyclin-E gene and protein in pregnant mice were steady increasing since day 2 and reaching maximal level on day 5 of pregnancy.Injection of Anti-cyclinE antibody could decrease the numbers of implanted blastocyst compared with that of saline-injected group.Conclusion:Cyclin-E were persistently expressed in mouse endometrium during the early pregnancy and might be concerned with proliferation differentiation of endometrium and participates in the regulation process of mouse blastodyst implantation.

Chemotherapy plays a significant role in cancer therapy,but chemotherapy-induced nausea and vomiting(CINV)common happened and affects patients satisfaction with treatment.With the development ot physiology on emesis,the new antiemetics such as 5-HT3 receptor antagonist(5-HT3RA),Aprepitant and Olanzapine greatly improved the control of CINV,the guidelines for antiemeties were deftned.Research progress of antiemetics was summarized.

Objective To investigate the quality of life (QOL) of patients with frequent premature ventricular contractions (PVCs) and the effects of radiofrequency catheter ablation (RFCA) on them.Methods 120 patients with frequent PVCs,100 cases with frequent APCs and 60 healthy persons were assessed by 36-Health survey questionnaire (SF-36) originally.Three months later,the QOL of 48 cases received RFCA and 72 cases with drug therapy were evaluated again by SF-36.Meanwhile,decrease of PVCs were also calculated between these two groups.Results The scores of physiological function and mental health in patients with PVCs were lower than that of cases with APCs,while the score of bodily pain in patients with PVCs was higher (P value:0.0002-0.0380).In addition,the quality of life in patients with frequent PVCs were worse than healthy control(P value:0.0001-0.0915).Three months later,PVCs in 48 cases with frequent PVCs received RFCA were much fewer than that of 72 cases with drug therapy (t=18.8682,P＜0.01),and the quality of life in the former was much better than the latter (F=15.329-39.274,P＜0.01).Conclusion Frequent PVCs can deteriorate the patients' quality of life.RFCA can control or eliminate PVCs in these patients and markedly improve the quality of life of them.

Aim To investigate the effect of angioten-sin 1-7 (Ang 1-7 )on the cardiac hypertrophy and my-ocardial fibrosis in deoxycorticosterone acetate (DOCA)-salt hypertensive rats and its possible mecha-nism.Methods Male Sprague-Dawley rats were used to establish DOCA-salt hypertensive model,which un-derwent uninephrectomy surgery and were subcutane-ously injected with a DOCA,and replaced drinking water with 1% saline solution for 4 weeks.DOCA-salt animals were implanted with osmotic minipumps, which delivered Ang 1-7 chronically for 4 weeks (200 ng ·kg-1 ·min-1 ).Arterial blood pressure,left ven-tricular function in rats,the area of myocardial cells in HE stained specimens,and the area of myocardial fi-brosis Sirius red staining specimens were measured. Real time PCR was used to detect the expression of at-rial natriuretic factor (ANF ) and β-myosin heavy chain (β-MHC)mRNA in heart,and TGF-β1 protein expression was observed by Western blot in myocardial tissue.Results In the first week,DOCA salt rat had a significant increase in arterial blood pressure,and reached a peak in the fourth week;while the left ven-tricular end-systolic pressure (LVESP),left ventricu-lar end-diastolic pressure (LVEDP ) and ventricular contraction the maximum rate of pressure rise (+dp/dt)had also undergone significant changes in DOCA salt rats. After chronic infusion of Ang 1-7 for 4 weeks,the arterial pressure,LVESP and LVEDP were significantly reduced and +dp/dt were increased sig-nificantly in DOCA salt rats (P<0.05 ,n=7 ).Ang 1-7 significantly reduced the cardiac index and myocar-dial cell area,as well as the up-regulated expression of ANF and β-MHC mRNA in DOCA salt rats (P <0.05 ,n =7 ).Meanwhile,Ang 1-7 also significantly decreased the perivascular fibrosis and interstitial fibro-sis area, and significantly inhibited the increase of TGF-β1 expression in DOCA salt rats (P<0.05 ,n=7 ).Conclusion These results indicate that Ang 1-7 has a cardioprotective effects through reducing arterial pressure and improving cardiac fibrosis and hypertro-phy in the DOCA-salt model of hypertension.

Urotensin-Ⅱ is a vasoactive 'somatestatin-like' cyclic peptide. Recently, human urotensin-Ⅱ has been cloned and demonstrated to be the most potent vasoconstrictor identified so far. The receptor of urotensin-Ⅱ has now been identified as the orphan receptor GPR 14 . This peptide may influence cardiovarscular homeostasis, pathology and also influence respiratory system, central nervous system and endocrine function.

OBJECTIVE:To raise the exigency capacity of drug system to cope with the unexpected events of public heal_ th.METHODS:To set up a national drug contingency system(NDCS)for the unexpected events of public health.RESULTS:The NDCS should consist of the reserves of drugs,capacity,information and plan for emergent needs.CONCLUSION:Estab?lishment of NDCS is a way to access health mordernization.

Objective To investigate the clinical pathologic features of ovarian malignant mesoderm mixed tumor (malignant mixed mesodermal tumor of uterus ,MMMT ) ,and its differential diagnosis ,clinical treatment and prognosis .Methods A retrospec‐tive analysis of the clinicopathological data of 9 patients with MMMT in our hospital from January 1985 to December 1985 was per‐formed ,and the clinicopathological data included routine paraffin section of HE staining and special staining and immunohistochemi‐cal study .Results Results showed that 8 cases were examined by ultrasound with side ovarian tumor ,1 case of patients with bilat‐eral ovarian tumors .Histological examination showed that there was 5 cases of endometrial carcinoma in epithelial components ,3 ca‐ses in clear cell carcinoma ,1 case in mucous adenocarcinoma .Composition of mesenchymal cells were fusiform or irregular shape , weaving or beam pattern ,or chaotic ,which 4 cases showed chondrosarcoma ,2 cases showed bone sarcoma component and glandular epithelial cell keratin and stromal elements vimentin (Cytokeratin ,CK) of 9 cases were + ,and estrogen receptor (estrogen recep‐tor ,ER) detection of 3 cases were + ,Progesterone receptor(PR) detection of 2 cases were + ,protein (desmin) of 2 cases were + ;highly glycosylation type I transmembrane glycoprotein (CD34) of 1 case was + .Conclusion Histological appearance is the main detection methods of ovarian malignant germ layer in the diagnosis of mixed tumor ,and B ultrasonic and CA125 inspection at the same time can effciently improve the accuracy of clinical diagnosis .

Objective To discuss the effect of the PDCA cycle in enhancing the case care ability of nurses in order for nurses to adapt to the assessment model of cases tracking.Methods The training was carried out for enhancing nurses' ability of case care according to the PDCA cycle.Results The scores of the case care,the quality of care in wards and the patient satisfaction degree increased significantly after applying the PDCA cycle compared with those before.The aims of ensuring medical safety,improving the work quality,and increasing patient satisfaction were achieved.Conclusions Training nurses' ability of case care following the PDCA cycle can effectively improve the nurses' ability of case care,make them adapt to the case tracking assessment model,which is worthy of promotion.

Dyslipidemia is a risk factor for stroke, and reducing low-density lipoprotein cholesterol (LDL-C) levels can decrease the risk of ischemic stroke. Both statins and PCSK9 inhibitors can effectively reduce LDL-C levels, but some patients cannot tolerate statins, and PCSK9 inhibitors are expensive. Animal experiments have shown that ezetimibe can reduce oxidative stress and endoplasmic reticulum stress response, inhibit cell apoptosis, increase autophagy, and protect nerve tissue by activating AMP activated protein kinase (AMPK) signaling pathway. Clinical studies have shown that ezetimibe can further reduce LDL-C levels on the basis of statin therapy, and reduce the risk of ischemic stroke in patients with acute coronary syndromes, and there is no obvious adverse reaction. However, ezetimibe cannot reduce the incidence of stroke in elderly people who are ≥75 years old, have elevated LDL-C and do not have coronary heart disease. At present, there are few studies on the use of ezetimibe for secondary prevention of ischemic stroke, and its effect needs to be further confirmed by large randomized controlled trials.

Primary nephrotic syndrome refers to one of the common glomerular diseases in children.Glucocorticoid is the first choice in the treatment of nephrotic syndrome, and the using cycle is long.Long term application of glucocorticoids can cause side effects, including Cushing′s disease, hypertension, decreased immune function, growth retardation and eye adverse reactions.Among eye adverse reactions, glaucoma and cataract are more common.Glucocorticoid-induced glaucoma and glucocorticoid-induced cataract are often concealed, and children often cannot accurately express their discomforts.At the same time, some clinicians lack the understanding of the adverse reaction, which is easy to cause missed diagnosis and misdiagnosis.Eventually, irreversible visual impairment occurred.Here, the etiology, pathogenesis, clinical diagnosis, and treatment of glucocorticoid-induced glaucoma and glucocorticoid-induced cataract are elaborated, thus aiming to improve clinicians′ understanding of glucocorticoid ophthalmopathy and the level of clinical diagnosis and treatment.