A novel H17N10 subtype of the influenza A viruses was found in bats in 2012. Protein sequence and structural analyses revealed that the HA17 and NA10 proteins of this strain are different from corresponding ones in known influenza A subtype viruses. Both HA17 and NA10 proteins cannot bind to sialic acid,which indicates that they may have novel functions. This article briefly describes the state of current research into the H17N10 subtype of bat influenza A virus.
Animals ; Chiroptera ; virology ; Influenza A virus ; classification ; genetics ; isolation & purification ; metabolism ; Orthomyxoviridae Infections ; veterinary ; virology ; Viral Proteins ; genetics ; metabolism

Human papillomavirus (HPV) is one of the most common infection of the virus in the reproductive tract,a growing number of studies found that HPV infection is closely related to female infertility.This paper mainly introduces the structure characteristics of HPV,the correlation of reproductive tract infection of HPV and infertility,the related factors of infertility by reproductive tract infection of HPV,the influence of early pregnancy and adverse pregnancy outcomes,the relationship with assisted reproductive technology,etc.

Objective To study the formulation and preparation of Qinglan Jingao tabales.Methods The orthogonal design was taken for the optimization,the contents were selected by granule,rigidity,crack and disintegration.Results The optimum dropping condition was as follow:the rate of Qinglan extraction to starch and dextrin was 25∶4∶1(kg),the added amount of 95%alcohol was 0.25 times(kg),mix 8 minutes by the wet granule compression technique.Conclusion The optimum extracting procedure is scientific,reasonable and stable.

Influenza poses a great threat to life and health in populations worldwide. Studies regarding the protein components of influenza viruses will facilitate the research and development of vaccines and diag nostic reagents. The influenza virus contains both structural and non-structural proteins. From the outset, it has been accepted that an influenza A virus possesses eight gene segments that encode eight corresponding viral proteins, respectively. Research has demonstrated that the M gene encodes the M2 ion channe! protein and the NS gene encodes the non-structural protein, NS2. In recent years, several novel viral proteins have been identified from influenza A viruses. This article will briefly describe the state of current research into PA-related proteins of influenza A viruses.
Animals ; Humans ; Influenza A virus ; enzymology ; genetics ; Influenza, Human ; virology ; RNA Replicase ; genetics ; metabolism ; Viral Proteins ; genetics ; metabolism

Background C57BL/6.NOD-Aec1Aec2 mouse is considered to be an idea model for the study of the pathogenesis of Sj(o)gren syndrome,but the cause of dry eye in these mice is unclear.Objective This study was to investigate the histopathological change of the ocular surfaces of C57BL/6.NOD-Aec1Aec2 mice,and to determine whether dry eye is developed spontaneously in these mice.Methods Forty-five clean C57BL/6.NOD-Aec1 Aec2 mice were used as the experiment group and forty-five C57BL/6J mice(both male and female)were used as the control group in this study.Detection of fasting blood-glucose,Schirmer' s test Ⅰ (S Ⅰ t),lissamine green staining and scoring of the corneal and conjunctival epithelium in the mice were performed at the age of 4,8,12,16 and 20weeks.Five mice from each group were sacrificed and their corneas were obtained to measure the central corneal epithelium thickness and to count the number of conjunctival goblet cells.In addition,lymphocyte infiltration in the lacrimal gland of the mice was examined with hematoxylin-eosin staining.The uhrastructure of the corneal epithelial cells and microvilli were assessed by scanning electron microscopy.The use and care of the mice were approved by the Experimental Animal Care Committee of the Third Military Medical University.Results No sign of dry eye was seen in both the 4-week-old C57BL/6.NOD-Aec1Aec2 mice and 4-week-old C57BL/6J mice.The S Ⅰ t values in 8-week-old,12-week-old,16-week-old and 20 week-old mice from the experiment group were (2.7 ±0.9) mm,(2.5 ±0.8) mm,(1.8±0.6) mm and (1.9± 0.1) mm,respectively,showing a significant reduction in comparison with those of the control mice of the same age(all P＜0.01).The amount of lissamine green staining in the C57BL/6.NOD-Aec1Aec2 mice gradually increased with age,showing elevated scores in 12-week-old,16-week-old and 20-week-old mice in the experiment group(all P＜0.01).The central corneal epithelium thicknesses were(20.18±3.75)μm,(17.01 ±5.25) μm,(14.19±5.72) μm and(12.00±3.25) μm in the 8-week-old,12-week-old,16-week-old and 20-week-old C57BL/6.NOD-Aec1Aec2 mice,respectively,which were significantly lower than those of the C57BL/6Jmice of the same age (all P＜0.01).The numbers of conjunctival goblet cells were (8.2±2.4),(6.2±2.1),(6.1 ±2.2) and (4.1 ± 2.0) in the 8-week-old,12-week-old,16-week-old and 20-week-old C57 BL/6.NOD-Aec 1Aec2 mice,respectively,showing a gradual decrease with age and a significant decline in comparison with those of the C57BL/6Jmice of the same age(all P＜0.01).Lymphocyte infiltration in the lacrimal gland and destruction of gland ducts were seen by hematoxylin-eosin staining,and acinar abnormality aggravated with aging.Reduction of corneal epithelial cells and the number of microvilli were distinguished with aging under the scanning electron microscope.The fasting bloodglucose levels of the two groups were both less than 6.0 mmol/L,and no significant difference was found between them at any age(P=0.637,0.610,0.163,0.086,0.938).Conclusions C57BL/6.NOD-Aec1Aec2 mice develop dry eye spontaneously with aging.The course of disease and characteristics of dry eye in C57BL/6.NOD-Aec1Aec2mice is similar to human dry eye.The C57BL/6NOD-Aec1 Aec2 mouse is the perfect model to study the pathogenesis of dry eye.

Cell cycle-related kinase (CCRK) as a novel CDK-activating kinase plays a significant role in cell cycle regulation.Related studies confirm that CCRK is overexpressed in various tumors,and is associated with poor prognosis of the patients,which indicates that CCRK takes part in the generation and development of the tumor.

The epiphysis is an unique structure in pediatric skeletons,which mainly reflect in its unique blood supply,anatomical structure,pathological basis and so on.Epiphyseal injury is common in children with skeletal injury.Fracture,tumor,infection can cause epiphysis injury,and inappropriate treatment is often easy to cause growth obstacle and skeletal deformity.Its treatment is relatively difficult.At present,there had not yet formed a consistent therapeutic strategy.Aiming at the pathobiologic basis,causes,mechanism and clinical manifestation of pediatric epiphyseal injury,now,its diagnosis,therapeutic strategy,the principle of treatment and the latest research progress were summarized,which would provide useful guidance for clinical treatment of pediatric epiphysis injury.

Objective This article is to explore the application of hospital in the new pattern of elderly health care service.Methods It takes 115 elderly who come from elderly health center as respondents , using questionnaire and inter-view, to study their satisfaction with the new pattern of elderly health care service , i.e.the combination of nursing , support and treatment , their living quality , happiness index and monthly average hospitalization expenses before their going to a nurs -ery and after.Results Their satisfactions are greatly increased after going to a nursery .Their living quality and happiness index are also higher when they go to a nursery (P<0.05, P<0.01), and the hospitalization expenses are lower after their going to a nursery (P<0.01).Conclusion Applying hospital in the new pattern of elderly health care service , elderly′s satisfactions with the new pattern of elderly health care service have been greatly increased , so have their living quality and happiness index .Meanwhile , the hospitalization expenses are also greatly decreased .The scope of the community has a cer-tain influence , and it is worth promoting .

OBJECTIVE:To obverse the efficacy and safety of Liuwei dihuang pill combined with TCM prescription acupoint application in the treatment of type 2 diabetic constipation. METHODS:120 patients with type 2 diabetic constipation were random-ly divided into control group(60 cases)and observation group(60 cases). All patients received controlling blood pressure and regu-lating blood lipids drugs,diet control combined with exercise,and fixed dose of Metformin hydrochloride tablet(0.5 g/times,2 times a day)and Acarbose tablet(100 mg/times,3 times a day);based on it,control group received 5 mg Mosapride citrate tablet, 3 times a day;observation group additionally received 8 Liuwei dihuang pills(3 times a day)combined with TCM prescription acu-point application (in double Tianshu Point,double Yongquan point,double Shenque Point and double Zusanli). 7 d was regarded as a treatment course,and it lasted 3 courses. Clinical efficacy,blood glucose [2 h postprandial glucose(2 h PG),blood glucose (FPG)],the clinical symptom indexes(defecation interval time,each defecation time,weekly defecation frequency)before and af-ter treatment,and the incidence of adverse reactions in 2 groups were observed. RESULTS:The total effective rate in observation group was significantly higher than in control group,and the difference was statistically significant (P<0.05). Before treatment, there were no significant differences in the 2 h PG,FPG,defecation interval time,each defecation time and weekly defecation fre-quency between 2 groups(P>0.05). After treatment,2 h PG and FPG in 2 groups were significantly lower than before,and obser-vation group was lower than control group;defecation interval time and each defecation time were significantly shorter than before, and observation group was shorter than control group;weekly defecation frequency was significantly higher than before,and obser-vation group was higher than control group,The differences were statistically significant (P<0.05). During treatment,6 patients showed adverse reactions in control group,and 5 in observation group,and there was no significant difference in the incidence of adverse reactions between 2 groups(P>0.05). CONCLUSIONS:Liuwei dihuang pill combined with TCM priscription acupoint ap-plication shows good efficacy in the treatment of type 2 diabetic constipation. It can effectively improve constipation,reduce defeca-tion interval time and each defecation time,increase weekly defecation frequency and have no influence on blood glucose control with small adverse reactions and high safety.