Female ; Humans ; Polycystic Ovary Syndrome ; drug therapy

Purpose To analyze the 64-row spiral CT manifestations and evaluate the diagnostic value in early primary ureteral carcinoma.Materials and Methods Fifteen cases of early primary ureteral carcinoma proved by surgery and pathology were reviewd.Unenhanced scan,enhanced scan in arterial phase,parenchymal phase,delayed phase and CPR construction were performed in all patients.MIP were performed in 10 cases.Results ① the localization of lesions:7cases were located at the right ureter and 8 cases at the left.2 cases were located at the upper portion of the ureter,6 at mid portion,and 7 at lower portion;② CT manifestations:8 cases showed irregular thickened wall and central lumen stenosis,4 cases eccentric soft tissue mass in lumen and crescent or linear vestigial lumen,3 cases soft tissue density and obsolescent lumen;③the enhanced features:the tumor was demonstrated apparent and uniform enhancement in 13 cases,inhomogeneous enhancement in 2 cases after administration of contrast medium;④ the accompanied signs:proximate renal and ureteral presented distention,dropsy in different degrees,5 cases appeared delayed development.Conclusion 64-row spiral CT volume scan and 3D reconstruction with multiplane and multiorientation,combined with axial enhanced scan,can show lesions dimensionly and increase the dignostic accuracy in early primary ureteral carcinoma,which has an important value.

Carrying out targeting an oncolytic adenovirus strategy in gene therapy of pancreatic cancer is a new direction.Currently,related research including vitro test and animal models vivo test,targeted strategies on molecular biology and gene level are carried out.Some of oncolytic adenovirus drugs have entered phase Ⅲ clinical trials.Oncolytic adenoviruses alone or in combination with other treatments,can enhanced anti-tumor effect.

Polycystic ovary syndrome (PCOS) is a disease of dysgenesia and dysmetabolism. Its clinical manifestations include hyperandrogenism, ovulation failure, hyperinsulinemia, elevated serum LH level, obesity and sterility. Histological examination of ovary reveals polycystic changes. It is difficult to carry out clinical research because of its complicated pathogenesis, pathophysiology and multiple biochemical changes and clinical manifestations. It is necessary to study PCOS using animal models. In this article, the authors introduce some methods of establishing animal models of PCOS, such as androgen administration (including testosterone propionate,dehydroepiandrosterone), combination of insulin with HCG, use of estradiol valerate,combination of progestrone with HCG, aromatic cyclase inhibitor method,and so on, and review the effects of traditional Chinese medicine on the intervention of PCOS.

Objective To investigate morphologic, structural and functional characteristics of cardiomyocytes from neonate rats and to set up a desirable technique for isolating and purifying the cardiomyocytes from neonate rats. Methods Using trypsin-digestion, mechanical separation, twice seedings and bromodeoxyuridine (BrdU)-treatment, the cardiomyocytes were isolated and purified from the hearts of neonate rats at 1-7 days after birth. Shapes and spontaneous pulsation of the cells were viewed. The cells were identified with cardiac isoform of tropnin T(cTnT) immunostaining. Ultrastructural features of the cells were examined with in situ transmission electron microscopy. Responses of the cells to adenine and isoprenaline were also examined. Results More than 95% cells isolated from the hearts of neonate rats are cardiomyocytes. The vital cells are more than 95%. Neonate rat cardiomyocytes include short columnar or rhabdoid cells and irregular cells. The most rhabdoid cells from the rats at 1-3 days after birth present the ultrastructural features of immature cardiomyocytes. The rhabdoid cells from the rats at 6-7 days after birth have some ultrastructural features of mature cardiomyocytes. Comparing with the cells at 1-3 days after birth, cTnT expression in the cells is slightly enhanced, the transverse striation was obvious. The irregular cells contain less bundles of myofilaments, the filaments are arranged irregularly. There are a few small cells which are in undifferentiated state. More than 80% cells show spontaneous pulsation at 72 hours after incubation. After treatment with adrenine and isoprenaline, the number of the cells with spontaneous pulsation increases and the intension of spontaneous pulsation is enhanced. The responses of the rhabdoid cells from the rats at 6-7 days after birth to adenine and isoprenaline are much stronger. Conclusion There are two kinds of neonate rat cardiomyocytes. They are different in ultrastructures, spontaneous pulsation and responses to adenine and isoprenaline. The cardiomyocytes from rats at 6-7 days after birth are suitable for experiments in vitro as mature cardiomyocyte. The method set up in this experiment is desirable for culture of neonate rat cardiomyocytes.

Objective　To investigate the alteration and clinical significance of NK cell and T lymphocyte subpopulatien in patients with rectal carcinoma. Methods　 The numbers of NK cell and T lymphocyte in 26 healthy persons and 35 rectal carcinoma patients were detected by 3H-releasing method and FCM. Results　NK cell and CD3, CD4, CD4/CD8 were decreased significantly in patients with rectal carcinoma (P<0.05), which was positively correlated with Dukes staging (P<0.01), and was inereased after redical or palliative operation (P<0.05), but NK cell and CD3, CD4, CD4/CD8 were further decreased in patients with unresectable tumor after operation (P<0.05). Conclusions　The immunity of rectal patients was decreased with the development of the disease, and it will be improved after radical of palliative operation.

This paper focused on the role conflicts owing to the multiple identity of roles and professional limi -tations in the dispute processing personnel , analyzed the probability in the medical processes and results , appeal of the patients and pattern of medical dispute processing , and made the conclusion that the medical dispute processing personnel is volunteers promoting social harmony and a practitioner of new medical model from the social perspec -tive and should carry forward the dedication , fraternity, mutual assistance and progressive spirit , the concept of the biological -psychology -social medical model to analyze the loss of patients in dispute and appeal , truly safe-guard the health rights and interests of patients , and give some advices to perfect the role ethics dispute processing .

Objective To explore the sensitivity and constancy of BNP in evaluating the therapeutic effect of congestive heart failure.Methods 80 patients made a definite diagnosis of congestive heart failure were selected and whose cardiac functional grading of NYHA,BNP and left ventricular ejection fraction (LVEF) were determined respectively pre-treatment,one week,one month and three months after-treatment.Statistical analysis on the difference of therapeutic effect was performed for each case.Results No statistical difference was found between the amplitude of BNP and LVEF in one week and on one month, three months pro-treatment by the scheme of captopril combining with metoprolol (P>0.05 ).But between the BNP and the cardiac functional grading,statistica difference was found in the fitst week pro-treament(P<0.05),when on one month and three months after treatment the statistical difference still not found(P>0.05).Conclusion BNP dynamic measuring had the hige sensitivity and constancy in quantitatively analysing the therapeutic effect of congestive heart failure which could be used as a reliable tool for evaluating the therapeutic effect of congestive heart failure.

of apoptosis-related gene.

The atlantoaxial articulation fracture or dislocation in children is one kind of common damage disease on upper cervical vertebra.Because the children are still in the stage of development,the atlantoaxial articulation ligamentum capsulare and the ligament are relax,so they are easy to be dislocated by external forces or be spontaneous dislocated caused by pathology reasons.The upper cervical vertebra damages on the children are quite different from that on the adults both in anatomical view and in biological term,therefore,the treatment are different from adults either.The treatments can be divided into the conservative ones and the surgery ones,the surgery treatment can also be divided into the posterior atlantoaxial fixed fusion technology,the occipitocervical fixed fusion technology and the odontid screw pexia.No matter using the conservative treatments or the surgical operation treatments,the root cure for the atlantoaxial articulation fracture or dislocation is to re-construct the stability of the articulation.The key point of freatment selection is to grasp the indications of each method.