Objective To analyze the clinical features and significance of the samples which the expression of ANA is positive and the expression of anti‐ENA antibody is negative .Methods Detect ANA and anti‐ENA antibody of all the 297 samples by indi‐rect immunofluorescence method and biological chip technology ,repectively .And analyze the fluorescence karyotype of ANA posi‐tive specimens with double‐blind method .Screen the samples which anti‐ENA antibody express negative from ANA positive sam‐ples .Compare and analyze the fluorescent karyotype .Results Of all the 297 samples ,74 cases were positive for ANA ,the positive rate was 24 .9% (74/297) .The majority of patterns were speckled pattern (43 cases ,58 .1% ) ,cytoplasmic granular pattern (9 ca‐ses ,12 .2% ) ,and antibody to centromere antigen(9 cases ,12 .2% ) ,respectively .In 74 cases of ANA positive specimens ,ENA anti‐body the negative rate of ENA antibody is 17 .6% (13/74) .Of the 13 cases ,the most frequently occurred pattern was the speckled pattern(11 cases ,84 .6% ) ,the rate of cytoplasmic granular pattern and antibody to centromere antigen was 7 .7% (1/13) respec‐tively .Conclusion In the serum samples which express ANA positive and ENA antibody negative ,the rate of speckled pattern is significantly higher than the other patterns ,and far more than the rate of speckled pattern in the samples which express ENA anti‐body and ANA positive (52 .5% ) (chi‐square= 5 .018 ,P< 0 .05) ,with statistical significance .We consider the speckled pattern samples be in favour of discovering new antibodys of this kind of serum sample .

ObjectiveTo observe the clinical efficacy of electroacupuncture plus magnetic resonance in treatingpostoperative gastroparesis syndrome.MethodPatients with postoperative gastroparesis syndrome were recruited and coded according to the onset time, and then randomized into a treatment group of 10 cases and a control group of 10 cases. The treatment group was intervened by electroacupuncture plus magnetic resonance, while the control group was by electroacupuncture. The recovery time of gastrointestinal motility and drainage volume of gastric fluid were observed after treatment.ResultThe drainage volume of gastric fluid was significantly reduced (P<0.05) and it took less time to recover the gastrointestinal motility in the treatment group;compared to the control group, it took a significantly shorter time to recover the gastrointestinal motility in the treatmentgroup (P<0.05); after 20-day treatment, the drainage volume of gastric fluid was markedly reduced in the treatment group (P<0.05). ConclusionElectroacupuncture plus magnetic resonance can produce a more significant therapeutic efficacy in treating postoperative gastroparesis syndrome thanelectroacupuncture alone.

Critical Discourse Analysis ( CDA) attempts to uncover the inequality in social reality and the way language presents power and ideology through the analysis of language features. Systemic functional linguistics ( SFL) focuses on the dialectical relationship between language and society and has been considered an important approach and theoretical basis to CDA. Doctors in Chinese medical clinic follow the Four Diagnostic Methods, within which the inquiry diagnosis strongly shows the fact that doctor and patient clash over the ideology and strug-gle for the power via language. The study carries out CDA on doctor-patient discourse in Chinese medical clinic within the theoretical framework of SFL from four aspects including nominalization, classification, transitivity and mood, which verifies that the language is the product of asymmetric power between doctor and patient. It is hoped that the paper would provide insight into CDA on doctor-patient discourse and would be of theoretical meaning.

Objective: Based on the same clinical laboratory automatic blood coagulation analyzer method comparison of different brands. Discuss different between blood coagulation analyzer test results are comparable or check whether the deviation of the results in the permitted range. Method:collecting 112 cases of fresh plasma specimens, using ACL ADVANCE and Sysmex CA7000 two kinds of blood coagulation analyzer, respectively prothrombin time (PT), activated partial prothrombin time (APTT), thrombin time (TT) and fibrinogen (FIB), and compare the results, to medical decision level of judging system error detection system for clinical result, and calculate the two blood coagulation instrument testing results of the correlation coefficient and linear regression equation. Results:the ACL ADVANCE and Sysmex CA7000 blood coagulation analyzer test of PT, APTT, TT and FIB result comparison, there were no statistically significant difference (t=0.974, t=0.649, t=0.765, t=1.163;P>0.05), the correlation coefficient respectively;Medical decision level of system error were less than half the total margin of error. Conclusion:to the ACL ADVANCE as standard test system, Sysmex CA7000 determination results have clinical acceptability, two blood coagulation test results with good correlation. Should be conducted on a regular basis in the same laboratory coagulation analyzer compares different experiments, to ensure the comparability of the same in the laboratory test results.

Objective To observe the clinical effect of umbilical cord blood stem cell transplantation in the treatment of decompensated hepatic cirrhosis .Methods The umbilical cord blood of healthy puerperal women was collected,and stem cells were isolated and injected into liver through hepatic artery to treat 20 patients withdecompensated cirrhosis.Blood,liver function,coagulation changes ,indicators of clinical symptoms and adverse events were observed after transplantation 1 week,2 weeks,4 weeks and 8 weeks.Results 1 week after transplantation ,the fatigue,loss of appetite situation were improved in more than 80% patients,but the changes were not significant ( t=0.88,0.80, 0.20,0.23,0.56,all P>0.05).2 weeks after transplantation,the bloating of 60% patients was improved,ALB in-creased significantly(t=2.03,P<0.05).After 4 weeks,the bloating of 70%patients relieved,50%of patients with ascites subsided.ALB,ALT,PT were significantly improved (t=3.84,P <0.01;t =1.75,3.02,all P<0.05);8 weeks later,75%of patients with ascites subsided .Except for AST was not obvious ,other indicators were improved significantly(t=2.20,2.22,5.93,all P<0.05).Except for one case had low fever ,there were no other adverse reac-tions.Conclusion Cord blood stem cell transplantation in the treatment of decompensated cirrhosis can achieve a certain short-term treatment effects , especially in increasing the level of ALB and reducing PT , could resume liver damage and has relatively high security at the same time .

Objective To investigate the skull base meningiomas communicate through the superior orbital fissure cranio-orbital approach of microsurgical Methods and the efficacy.Methods 80 cases with skull base meningiomas were randomly divided into the control group and observation group,40 cases in each group.The control group was given contact microsurgery,and the observation group received communication through the superior orbital fissure cranio-orbital approach of micro-surgery.The improvement of symptoms and postoperative complications were compared and analyzed.Results (1)The operative time,blood loss,blood transfusion,Simpson grade (Ⅰ,Ⅱ,Ⅲgrade),DKPS,postoperative hospital stay and mortality rate in the control group were (9.28 ± 0.97)h,(1 222.38 ± 89.07) mL,(1 099.35 ±56.72) mL,14/17/9,3.21 (0,6.44),(29.78 ±3.29)d and 7.50％ (3/40),which in the observation group were (7.02 ± 0.65) h,(877.34 ± 44.50) mL,(677.10 ± 32.28) mL,29/9/2,3.26 (-3.33,10),(20.00 ± 2.75) d and 5.00％ (2/40).The differences of operative time,blood loss,blood transfusion,Simpson level,postoperative hospital stay between the two groups were statistically significant(t =4.209,4.997,5.823,x2 =4.011,t =5.711,P =0.033,0.027,0.018,0.022,0.013),but the D KPS and mortality between the two groups had no significant differences (t =0.433,0.096,P =0.089,0.317).The monocular prominent,eye movement disorder and vision improvement rates in the control group were 66.67％,72.22％ and 75.68％,which were significantly lower than 92.31％,94.12％ and 97.14％ in the observation group (x2 =5.932,4.381,6.793,P =0.027,0.033,0.020).The postoperative eye movement disorder,on ptosis,eye abduction,subcutaneous fluid and intracranial infection rates in the control group were 10.00％,15.00％,30.00％,12.50％ and 7.50％,those in the observation group were 7.50％,5.00％,10.00％,10.00％ and 7.50％.The postoperative eye movement disorder,subcutaneous fluid and intracranial infection rates had no significant differences between the two groups(x2 =0.923,1.033,0.785,P =0.387,0.595,0.233).The incidence rates of postoperative ptosis,abduction eyeball had statistically significant differences between the two groups (x2 =6.299,7.889,P =0.018,0.009).Conclusion The skull base meningiomas communicate through the superior orbital fissure cranio-orbital approach of microsurgery has significant effect than the conventional contact microsurgery,which deserves to be promoted in clinical.

Objective: To explore the path selection of national obligations in realizing the social security rights. Methods:Exemplification is applied to explore the problems in the realization of social security rights through the analysis of the specific circumstances of the operation of the New Rural Cooperative Medical System(NRCM). Results: Through analyzing the problems in the realization of social security rights under NRCM in China, the realization progress of social security rights not only have imperfections in legal system, but also have problems in insufficient fund and incomplete system, which lead to the low social security level of residents. Conclusion: To improve the realization of residents’ social security rights, the specific pathway was analyzed according to the pathway selection of fulfilling the China National Obligations. Pathway 1: enhance the legislation, implement the legal system of social security; pathway 2: based on the function display of administrative organ, enhance the supervision of administrative management; pathway 3: based on judicial remedy, secure the realization of social security right.

Following the realization that involved in all of the key process within the cell nucleus such as DNA replication,transcription,reorganization,repair,human DNA topoisomerase Ⅰ(Topo Ⅰ) is a useful therapeutic target against tumor growth.Topo Ⅰ inhibitors represent a class of effective agents that have been extensively exploited and used for carcinomachemotherapy.Current Topo Ⅰ inhibitors being clinically used or developed are classified as campotothecins and non-camptothecins.This presentation introduces current status and trends of Topo Ⅰ inhibitors as anti-tumor agents with an emphasis on bioactive and pharmacological properties of these agents.

Radioiodine 131 treatment for Graves' hyperthyroidism began nearly 70 years ago,and it has been recognized as the mainstream therapy for Graves' hyperthyroidism in western countries due to its simplicity and high cure rate.Indications of radioiodine 131 treatment have been changing during the past decades,now its main indication is Graves' hyperthyroidism of adult patients,especially when the patients were complicated with heart disease,leucocytopenia or hepatic damage.In Western countries,the cure rate of radioiodine 131 treatment is approximately 100%,yet the incidence of hypothyroidism is above 50%.Now hypothyroidism is accepted as an inevitable outcome for many patients after radioiodine 131 treatment.Evidence-based experiments during the past 70 years also showed that the therapy did not increase incidences of malignancies or leukemia,and had no influence on genetics or reproduction.

Objective To study the migration of neuronal precursors around the infarct area after focal cerebral ischemia.Methods Animal models of middle cerebral artery occlusion(MCAO) were established in 16 adult rats,and four of another 8 rats served as normal control and four underwent sham operation.Immunohistochemistry and cell count were applied to explore and contrast the migration of neuronal precursors for up to three weeks after a 2-hour episode of focal cerebral ischemia.Results The migration of neuronal precursors toward the infarct area could be found at various timepoints.The number of neuronal precursors started to increase in 1 d after reperfusion around the infarct area and reached the peak in 7 d,then declined till day 21.However,the neuronal precursors could not be examined in the corresponding non-ischemia area.Conclusion The neuronal precursors could migrate into the ischemic penumbra.It indicates that after cerebral ischemia,neuronal precursors are directed toward the sites of brain injury,where they might be in a position to participate in brain repair and functional recovery.