OBJECTIVETo investigate the clinical efficacy of Ginkgo biloba injection combined with traditional therapy in the treatment of explosive deafness.

METHODSFifty-six (98 ears) participants who were all coal miners were selected. These patients were diagnosed with explosive deafness. and admitted to the otolaryngology department of our hospital from May 2010 to October 2013. They were.equally divided into control group and treatment group according to the therapeutic method. The patients in the control group only received traditional therapy, such as hyperbaric oxygen, while the patients in the treatment group were given Ginkgo biloba injection in addition to the therapy for the control group. The improvement in hearing was recorded and compared between the two groups.

RESULTSThe rate of improvement in tinnitus symptom in the treatment group was 78.0% (32/41), significantly higher than that in the control group (55.6%, 25/45) (χ2=4.857, P<0.05); compared with the treatment group (93.5%, 43/46), the control group had a significant lower overall response rate at 76.9% (40/52) (χ2=5.160, P<0.05).

CONCLUSIONCompared with traditional therapy alone, combined ginkgo biloba extract injection and traditional therapy can significantly improve tinnitus symptom and overall response rate in the treatment of explosive deafness in coal miners, which suggests that the combination therapy is worthy of clinical application.

Coal Mining ; Combined Modality Therapy ; Deafness ; drug therapy ; Explosions ; Ginkgo biloba ; chemistry ; Hearing Tests ; Humans ; Injections ; Noise, Occupational ; adverse effects ; Oxygen ; therapeutic use ; Phytotherapy ; Plant Extracts ; therapeutic use ; Tinnitus ; drug therapy

Vitamin E( VE) is a major human plasma fat-soluble antioxidant. It has been proved that VE can protect the nervous system,skeletal muscle,retina and the cardiovascular system,etc. Nowadays,more and more researchers focus on the relationship between vitamin E and asthma. This article reviewes the research pro-gresses of the physiochemical properties of VE,and its relationship with asthma.

Retained products of conception (POC) complicates nearly 1% of all pregnancies, occurring with greater frequency after termination of pregnancy than after vaginal or caesarean delivery. The presenting symptoms of retained products of conception are similar to those of gestational trophoblastic disease and hence accurate differentiation is difficult based on clinical history and physical examination alone. The distinction between these two entities is extremely important as the treatment differs dramatically. These patients often need to be further evaluated with either ultrasound, computed tomography (CT) or magnetic resonance imaging (MRI) of the pelvis. Hence, radiologists play a vital role in clinching the diagnosis although at times it may be challenging to differentiate between these two entities. Herein, we discuss a case of degenerated retained products of conception which was initially misdiagnosed as invasive trophoblastic disease in a 41-year-old woman whom last known pregnancy was 10 years ago.

Objective To explore the changes of bone metabolic markers during early stage of preterm infants, as well as the relationship with their nutrition status. Methods Preterm infants with gestational age 30-35 weeks admitted to our Hospital were collected from November 2012 to April 2013. Venous blood samples obtained within 24 hours after birth and between 8:00-9:00 AM two weeks after birth were used to determine the Serum β-CTx, osteocalcin ( OC) and propeptide of type I procollagen (PINP) levels by Electro-Chemiluminescence. Analysis of changes of these bone metabolic markers and their relationship with early stage nutrition related indicators were also performed. Results A total of 60 premature infants were collected. There was no significant correlation among serum β-CTx, OC and PINP within 24 hours after birth ( r=0. 170, P＞0. 05 ) . The Serum β-CTx within 24 hours after birth was negatively correlated with gestational age (r= -0. 603, P＜0. 05), whereas the serum OC within 24 hours after birth was positively correlated with gestational age ( r=0. 581, P＜0. 05 ) . However, PINP wasn′t correlated with gestational age significantly (r=0. 134,P＞0. 05). Serumβ-CTx, OC and PINP at 2 weeks after birth increased significantly from the baseline level detected within 24 hours after birth ( P＜0. 05 ) .Δβ-CTx was positively correlated with ΔOC (r=0. 600,P＜0. 05). There was no significant correlation between ΔPINP and Δβ-CTx (r=0. 045,P＞0. 05), as well as ΔOC and ΔPINP (r=0. 110,P＞0. 05).ΔOC was positively correlated with average daily calorie ( P＜0. 05 ) and average daily P/E ( P＜0. 05 ) , negatively correlated with cumulative loss of caloric ( P＜0. 05 ) . There was no significant correlation between Δβ-CTx or ΔPINP with nutrition related indicators of this study. Conclusions Serum OC within 24 hours after birth of preterm infants and their gestational age are positively correlated, while β-CTx detected at the same time and gestational age are negatively correlated. Vigorous metabolism of premature bone occurs during the first two weeks after birth, as the serum β-CTx, OC and PINP levels increased significantly. We suggest that reasonable calorie intake and appropriate protein calorie ratio at early stage after birth is very important for the development of bone of preterm infants.

A lot of reports suggested that inducible nitric oxide synthase (iNOS) has a very different nature from constitutive NOS including endothelial NOS (eNOS) and neural NOS (nNOS). When exposed to cytokines or bacterial products, iNOS could be greatly activated and produces hundreds or thousands fold more NO than it does usually. Whether iNOS activation is arterial pressure related is not clear. In the present experiment, we studied three groups(n=6) of Sprague Dawley (SD) rats with implanted aorta and venous catheters that were maintained on 1 mEq/d, 12.5 mEq/d and 25 mEq/d of sodium intake respectively. Pulsatile arterial pressure signals from the amplifier were sent to a digital computer and the urine samples were taken every other day for nitrate/nitrite excretion (UNOx) assay using Greiss Reaction. After 6 days infusion, the rats were euthanized with an overdose of sodium pentobarbital, and the renal medullas were rapidly removed and frozen on dry ice for iNOS activity assay. Morever separate groups of hypertensive rats including spontaneously hypertensive rat (SHR, n=6) and High NaCl-induced hypertensive rat (NaHR, n=6) were used to measure renal iNOS protein by Western Blotting. The results showed that the mean arterial pressure (MAP) were significantly increased with the increase intake of sodium, the MAP (mmHg) at day 6 were 99.6±3.5,116.65±4.2 and 125.43±4.5, and the iNOS activity (nmol*g-1 protein*min-1) were 122.3±23.4, 342.4±35.6 and 623.9±65.4 in 1 mEq/d, 12.5 mEq/d and 25 mEq/d of sodium intake-rats respectively. At the same time, UNOx at day 6 were also increased, in turn, to 5 865.6±343.0 (for 12.5 mEq/d intake-rats) and (9 642.8±1 045.3) (for 25 mEq/d sodium intake-rats) nmol/d from (3 834.9±234.8) nmol/d of 1 mEq/d sodium intake-rats respectively. Western blotting showed that the renal medullary iNOS protein in SHR and NaHR were increased by 178%±13% and 104%±9% of normal Wistar rats. The data indicates that elevated arterial pressure could be an effective stimulus for iNOS activation.

Objective To study the possibility of endoscopic thyroidectomy. Methods Benign thyroid tumors, with diameter between 22 mm and 38 mm, were successfully removed in 8 patients by endoscopic thyroidectomy through anterior chest wall. Results The mean operation time was 120 min (range, 60 min～180 min), and the mean intraoperative blood loss was 20 ml (range, 10 ml～50 ml). No intraoperative or postoperative complications occurred. And no scars remained. Conclusions Endoscopic thyroidectomy through anterior chest wall is a safe and effective procedure for benign thyroid tumors, with excellent cosmetic results in the neck postoperatively.

Objective: To develop a type of oral biofilm to treat ora l ulcer.Methods:Chitosan film was prepared with chitosan only,comp osite chitosan film was prepared with chitosan(7 mg/cm 2),aloe (51 mg/cm 2),di caine(0.55 mg/cm 2) and EGF(40 IU/cm 2).Oral ulcer was made by local applicati on of crystal NaOH in 90 SD rats. The rats were divided into 3 groups with 30 in each. The oral ulcer in each group was treated by chitosan film(group C), compo site chitosan film(group CC) or without treatment(control). The ulcer healing wa s obsered by naked eye and examined pathologically. Results:The average healing time(d) of the ulcer in the groups of C,CC and control was 6.2 5 ? 1.29 ,5.58?1.3 and 8.75?2.49 respectively (C or CC vs Control P0.05). 2 days after treatment higher PCNA expression was observ ed in group CC than that in control(P

Objective To study the relationship between promyelocytic leukemia(PML) protein and apoptosis and their roles in the pathogenesis of psoriasis. Methods Immunohistochemical techniques were employed to study the expression of PML protein in the uninvolved skin, progressive plaque lesions and regressive lesions. Apoptosis was detected with terminal deoxynucleotidyl transferase-mediated dUTP-DIG nick end labeling(TUNEL). Results There was a significantly upregulated expression of PML protein in progressive plaque lesions, compared with that in uninvolved skin adjacent to the lesions and regressive lesions, and there was nearly no expression in normal skin; there was obvious apoptosis of keratinocytes in the epidermis with intensive PML protein staining. Conclusion The increased apoptosis of keratinocytes in the lesions of psoriasis induced by PML protein might be a homeostatic mechanism to the hyperplasia of keratinocytes.

Chinese materia medica (CMM) have many unique characteristics, such as complex chemical compositions, large differences in properties, and difficulty in separation. Pharmacists try to use various analytical methods to analyze the chemical compositions in CMM. With the development of liquid chromatography technology, the ultra-high performance liquid chromatography (UPLC) has been applied in the analysis field of CMM, and further promoted the in-depth study of CMM. This paper summarizes the development history of HPLC and UPLC, describes its current application in the analysis field of CMM, and provides an outlook of the future development of UPLC. The purpose of this paper is to promote the more reasonable and wider application of UPLC in the field of CMM analysis.

Objective: To establish a simultaneous quantitative analysis method of 58 pesticide residues in Anemarrhena asphodeloides based on QuEChERS-GC-QQQ-MS/MS technique. Methods: This study was performed on a Shimadzu GC-QQQ-MS/MS spectrometer, equipped with a SHIMADZU SH-Rxi-5Sil MS column (30 m × 0.25 mm, 0.25 μm). The temperature of injection port was 250 ℃, the injection volume was 1.0 μL, the injection mode was splitless, the injection pressure was 250 kPa; The carrier gas was high purity helium and the carrier gas control mode was constant linear velocity mode; The column flow rate was 1.69 mL/min, the line speed was 47.2 cm/s, the purge flow rate was 5 mL/min. The temperature rise method was the gradient program: The initial temperature was 50 ℃, hold the state for 1 min, first raise the temperature to 125 ℃ with 25 ℃/min, then raise the temperature to 300 ℃ with 10 ℃/min, keep the state for 15 min; The balance time was 0.5 min. The QuEChERS method was used to purify the test sample solutions, and the calibration was carried out using the standard curve of blank matrix matching, using a stable isotope internal standard for quantitative. Results: Quantitative determination of 58 pesticide residues in 30 batches of A. asphodeloides samples was carried out. The results showed that there were five batches of samples detecting a small amount of p,p’-DDE, three batches of samples were detected trifluralin, two batches of samples were detected chlorpyrifos, and one batch of samples were detected butralin. Conclusion: The development and validation quantitative analysis method of 58 pesticide residues based on QuEChERS-GC-QQQ-MS/MS has good applicability, which is of reference value for the establishment of other pesticide residues in herbs.