Objective To evaluate the relationship of serum cystatin C (Cys C) levels and the severity of coronary artery diseases (CAD).Methods A total of 194 patients underwent coronary angiography were divided into two groups (102 CAD patients) and control group (non-CAD group,92 cases).To study the relationship of the level of serum cystatin C and the severity of CAD,the serum cystatin C of all enrolled patients was detected by immune-enhancing latex turbidimetry and the serum cystatin C level of CAD group was compared to control group.Results The serum levels of cystatin C of CAD were significantly higher than the control group (P ＜ 0.05).With the increased levels of serum Cys C,CAD severity average score was increased; logistic regression analysis showed that Cys C was one of the risk factors of CAD.Cystatin C concentration was significantly correlated with CAD severity score.A cut-off value of 0.79 mg/L for cystatin C predicted incident CAD with a sensitivity of 76.60％ and specificity of 82.60％,respectively.Conclusions Serum Cys C levels are valuable clinical predictors and closely related to CAD.With the increasing levels of serum Cys C,CAD becomes more severe.

Objective: To study the relationship between FAT10 expression and biological behaviors in inif trating ductal carcinoma of breast. Methods: The expressions of diubiquitin (FAT10), estrogen receptor (ER), progesterone receptor (PR) and c-erbB2 in 50 cases of inif trating ductal carcinoma of breast were detected by immunohistochemistry. Western blot was used to detect FAT10 expression in MB-MDA-435, MB-MDA-435-transfected with FAT10 siRNA expression plasmid, MCF-7 and MCF-7-transfected with FAT10 expression plasmid, respectively. Transwell was used to detect invasion capability of MB-MDA-435, MB-MDA-435-transfected with FAT10 siRNA expression plasmid, MCF-7 and MCF-7-transfected with FAT10 expression plasmid. Results: hTe expression intensity of FAT10 was signiifcantly correlated to patho-grading, lymph nodes metastasis, distant metastasis and TNM staging (P<0.01), but not to age of patients and tumor sizes in iniftrating ductal carcinoma of breast (P>0.05). hTe expression intensity of FAT10 in receptor- negative group was obviously stronger than that in receptor- positive group (P<0.01). hTe expression intensity of FAT10 in triple-negative breast cancer was signiifcantly stronger than that in non- triple-negative breast cancer (P<0.01). hTe survival rate of patients with FAT10 positive expression was significantly lower than negative ones (P<0.05). Western blot results showed that FAT10 intensity in MB-MDA-435 significantly higher than that in MCF-7. Up-regulation expression of FAT10 could obviously increase the invasion capability of MCF-7, and down-regulation of FAT10 could signiifcantly decrease the invasion capability of MB-MDA-435 (P<0.01). Conclusion: FAT10 might increase the invasion capability of breast cancer cells by direct or indirect ways, and play an important role in invasion and metastasis of breast cancer. FAT10 might be an independent index for evaluation of breast cancer prognosis, and a potential target for breast cancer therapy, especially for triple-negative breast cancer.

BACKGROUND:Biliary stent restenosis is one of the main long-term complications following biliary stent restenosis implantation,which affects its application.OBJECTIVE:To evaluate the reasons for the biliary stent restenosis and to explore corresponding prevention methods.METHODS:A computer-based online search of MEDLINE,Cochrane library,CNKI and VIP between January 1989 and August 2009 was performed to search related articles with the key words of "biliary metal stent,reobstruction,and interventional therapy".Literatures related to biliary stent restenosis were selected,in the same field,the articles published lately or published by authoritative journals were preferred.A total of 171 articles were searched and 22 documents were included according to inclusion and exclusion criteria.RESULTS AND CONCLUSION:Tumor growth,gallstone formation and inflammatory hyperplasia are the main reasons for restenosis following biliary metal stent implantation.The interventional therapy is an effective way for the patients of stent restenosis.Simultaneously,the therapeutic efficacy and living quality of patients can be improved by noticing preoperative conditions,in-time detecting and controlling biliary infection,reducing complications as well as strengthening postoperative combined therapy.

BACKGROUND:It generally thought that based on cholangiography,biliary tract stents implantation can achieve a same long-term therapeutic efficacy as resection,and obtain good clinical outcomes in treating hilar cholangiocarcinoma when combined with percutaneous portcatheter system.OBJECTIVE:To evaluate the clinical methods of double interventional therapy and the biocompatibility between stent and host in hilar cholangiocarcinoma following stant implantation.METHODS:The databases of MEDLINE,Cochrane library,CNKI and VIP were retrieved with deadline of August 2009.Medium survival time and jaundice decrease rate,complication rate as well as biocompatibility between stent and host were sewed as assessment index.Clinical studies addressing double interventional therapy for treating proximal bile duct cancer were included,and animal studies were excluded.A total of 156 literatures were obtained initially by computer,and 25 literatures were included in further analysis.RESULTS AND CONCLUSION:In vitro test showed that stents carrying antibiotic,silver,or Escherichia coil,which may prevent adhesion of bacteria,however,it obtained poor clinical results.Chitosan is a natural cationic polymer and possess good biocompatibility,antibiosis and biodegradability.For the compare of the therapeutic effectiveness among different surgical procedure,it found that the decrease of total bilirubin were more faster in the internal and external drainage group than the resection group,however,the difference had no statistically significance.According to the Kaplan-Meier survival analysis,the medium survival time of 7 patients in the resection group was 16 months.The 1,3,5 years survival rate were 57.1%(4/7),28.6%(2/7),14.3%(1/7),respectively.Among the double interventional therapy group,the medium survival time of 19 patients was 18 months,and the 1,3,5 years survival rate were 52.6%(10/19),27.8%(5/19),15.8%(3/19),respectively.The medium survival time was 12 months in 14 patients of the control group,with 1,3,5 years survival rate of 57.1%,28.6%,14.2%,respectively.The results demonstrated that biliary tract stents implantation combined with percutaneous portcatheter system can improve life quality and prolong survival time of patients with hilar cholangiocarcinoma.

Objective:To explore the role of peripheral blood lymphocyte (PBL) of the renal transplant recipients during the acute rejection phase by gene chips.Methods:The 8 patients with acute rejection (AR) after renal transplantation were collected peripheral blood before operation (as control samples) and renal biopsy (as experimental samples).By Ficoll method,PBL was collected.Total RNA were extracted by one-step technique and purified.The total RNA were labeled with Cy5-dUTP (experimental samples) or Cy3-dUTP (control samples),then to label the cDNA probe by reverse transcript way.The gene chip (419 genes) was hybridized and scanned.Then fluorescent signal value of gene expressing was obtained,and differential expression genes were sifted.Results:There were differential expression 49 immunological genes in peripheral blood lymphocyte (PBL) of the renal transplant recipients during the acute rejection phase,including up-regulated 25 and down-regulated 24.Conclusion:Peripheral blood lymphocyte was involved in various stages during the acute rejection,and immunosuppressants influenced on these stages in various degrees. [

Titanium is abundant, soft and widespread, moreover it costs lowly and contributes towards an excellent biocompatibility and causticity resistance. For the metal materials used for repairing human hard tissues, titanium is the most approach to the human hard tissues regarding elastic modulus. This result may relieve the mechanical inadaptability between metal implants and bone tissues. Compared to other alloys, titanium inlay or crown plays a role on the protection of dental pulp and the prevention of cold or hot stimulus. Titanium and titanium alloy are satisfactory with regrad to the physical, chemical and mechanical properties, especially the biocompatibility and elaboration. Those good characteristics lead to a wide application prospect of titaniun in the field of dental restoration. Titanium and titanium alloy have become a hot topic of the alloy study for the stomatology.

As an internal environment of tumor occurrence, tumor microenvironment is composed of a variety of cells and extracellular matrix, and plays a crucial role in tumor formation, transfer and resistance to drugs. The regulation of tumor microenvironment will be a potential target to control the cancer. MicroRNAs (miRNAs) are a kind of 21 to 25 nucleotides single-stranded RNA, and are mainly involved in regulating gene expression. Recently, with the suggestion of cellular auton-omous tumor inhibition mechanism, the regulation of tumor microenvironment by miRNAs has received great attention. This review summarizes recent findings on the non-cell-autonomous mechanisms of miRNAs-mediated regulation of tumor micro-environments, which provides foundations and perspective on the design of therapeutic interventions.

Glioblastoma (GBM) is one of the WHO gradeⅣmalignancies, which is an acentral nervous system cancer with poor prognosis unless the lesion is in the brain stem. The incidence of GBM accounts for 80%of human primary malignant tumors in brain. Only 5%GBM can survive up to 5-years. Many researches showed that Sox2 is a pluripotent regulator, and muta?tion or abnormal function of Sox2 are closely related to the development of GBM. There are studies demonstrated the possibil?ity of using Sox2 gene as apotential target for GBM therapy. This paper reviewed recent progress in GBM.

Objective To investigate the value of the lauromacrogol in sclerosis therapy of endometrial cyst models of SD rat.Methods 45 SD rats were randomly divided into lauromacrogol treatment group,dehydrated alcohol treatment group and physiological saline blank group after being built cysts models.Then corresponding medicine was injected into the cysts,and the changes of pathomorphology and VEGF expression of ectopia endomembrane were observed a week later.Results Microscope observation indicated that endometrial cyst glandular epithelium and gland were inactived by both lauromacrogol and dehydrated alcohol.The effective rate of lauromacrogol group was 87.5％,and that of dehydrated alcohol group was 85.0％.There was no significant difference between these two methods (P =1.000).The ectopia endomembrane glandular organs of physiological saline group were not destructed obviously.Besides,VEGF expression of lauromacrogol and dehydrated alcohol group were lower than physiological saline group (P =0.003 and 0.006).There was no significant difference between VEGF expression of lauromacrogol and dehydrated alcohol group (P =0.926).Conclusions Compared with dehydrated alcohol,lauromacrogol had the same therapeutic effect on sclerosis therapy of endometrial cyst models.

Objective To explore the effect of Bushencupailuan decoction , a Chinese medicine formula , on polycystic ovary syndrome ( PCOS ) and its mechanism through observing the sex hormones , anti-Müllerian hormone ( AMH) and the morphology of ovaries of PCOS rat models .Methods Bushencupailuan decoction was administered in DHEA-induced PCOS model rats .The pathology of ovaries was examined , and the serum sex hormones and AMH were detected.Results Compared with the normal group , the ovaries of model group rats had severe diffuse cystic dilatation and cystic cavities of different sizes .The granular layer cells were compressed into flat or cubical shapes .Corpora lutea and follicles in various stages of development were rare .Bushencupailuan decoction obviously improved the numbers of mature follicles and corpora lutea , levels of sex hormones and AMH of PCOS model rats compared with those of model rats ( P<0.05).Conclusions Bushencupailuan decoction can treat PCOS by improving the serum sex hormone levels in PCOS model rats, reduce serum AMH and help to restore ovulation .