1.RNF152, a novel lysosome localized E3 ligase with pro-apoptotic activities.
Songling ZHANG ; Wei WU ; Yanfang WU ; Jiyan ZHENG ; Talin SUO ; Hong TANG ; Jie TANG
Protein & Cell 2010;1(7):656-663
RING finger protein 152 (RNF152) is a novel RING finger protein and has not been well characterized. We report here that RNF152 is a canonical RING finger protein and has E3 ligase activity. It is polyubiqitinated partly through Lys-48-linked ubiquitin chains in vivo and this phenomenon is dependent on its RING finger domain and transmembrane domain. RNF152 is localized in lysosomes and co-localized with LAMP3, a lysosome marker. Moreover, over-expression of RNF152 in Hela cells induces apoptosis. These results suggest that RNF152 is a lysosome localized E3 ligase with pro-apoptotic activities. It is the first E3 ligase identified so far that is involved in lysosome-related apoptosis.
Amino Acid Sequence
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Apoptosis
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HeLa Cells
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Humans
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Lysosomes
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metabolism
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Molecular Sequence Data
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Phylogeny
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Proteasome Endopeptidase Complex
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metabolism
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RING Finger Domains
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Sequence Alignment
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Ubiquitin-Protein Ligases
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metabolism
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Ubiquitination