1.Penicillium Species Other Than Talaromyces marneffei Producing Red Pigment from Clinical Specimens: Isolation of Talaromyces albobiverticillius.
Mi Ra RYU ; In Young YOO ; Dong Joon SONG ; Hee Jae HUH ; Chang Seok KI ; Nam Yong LEE
Laboratory Medicine Online 2017;7(4):211-214
No abstract available.
Penicillium*
;
Talaromyces*
2.Occurrence and identification of Penicillium and Talaromyces species from beach sand
Teh Li Yee ; Nurul Farizah Azuddin ; Masratul Hawa Mohd ; Latiffah Zakaria
Malaysian Journal of Microbiology 2022;18(6):652-664
Aims:
Penicillium and Talaromyces were among the species of microfungi that inhabit beach sand in Batu Ferringhi Beach, Penang Island, Malaysia. Previously, Talaromyces was described as the sexual stage of Penicillium, but both are now accepted as separate genera based on molecular phylogeny. The aim of the present study was to identify species of Penicillium and Talaromyces that are present in beach sand in Malaysia.
Methodology and results:
Species identities were confirmed according to similarities of the internal transcribed spacer regions and β-tubulin gene sequences and a phylogenetic analysis based on both regions/gene. Nine Penicillium spp. were identified as P. georgiense, P. chermesinum, P. pimiteouiense, P. citrinum, P. oxalicum, P. daleae, P. rolfsii and Penicillium sp. and the four Talaromyces spp. were T. siamense, T. atroroseus, T. minioluteus and T. fusiformis.
Conclusion, significance and impact of study
These findings showed that beach sand harboured a variety of Penicillium and Talaromyces species. The occurrence of Penicillium and Talaromyces in beach sands is associated with the organic matter in the sand, which provides suitable substrates and nutrient sources. Due to this, beach sand might harbour many potentially pathogenic or opportunistic species that may pose a health concern to immunocompromised individuals.
Penicillium
;
Talaromyces
;
Sand
3.Diversity of Aspergillus, Penicillium, and Talaromyces Species Isolated from Freshwater Environments in Korea
Inbeom HEO ; Kyeongyeon HONG ; Hyejin YANG ; Hyang Burm LEE ; Young Joon CHOI ; Seung Beom HONG
Mycobiology 2019;47(1):12-19
In order to elucidate the fungal diversity and community structure in freshwater environments, numerous fungal strains were isolated from freshwater, submerged soils, twigs, dead insects, etc. Among them, the present study has focused specifically on Aspergillus, Penicillium, and Talaromyces species, which produce diverse useful metabolites in general. Twelve strains of Aspergillus isolated were identified as A. japonicus (n = 5), A. tubingensis (3), A. niger (2), and A. flavus (2), 10 strains of which belong to Aspergillus section Nigri, named black Aspergillus. Eight strains of Penicillium were identified as P. brasilianim (n = 3), P. oxalicum (2), P. crustosum (1), P. expansum (1), and P. piscarium (1). Two different strains of Talaromyces were identified as T. pinophilus and T. versatilis. Thus far, Penicillium piscarium and Talaromyces versatilis have been unrecorded in Korea, for which we provide detailed morphological and molecular characteristics.
Aspergillus
;
Fresh Water
;
Insects
;
Korea
;
Niger
;
Penicillium
;
Soil
;
Talaromyces
5.Diversity of Endophytic Fungi Associated with the Roots of Four Aquatic Plants Inhabiting Two Wetlands in Korea.
Young Hyun YOU ; Jong Myong PARK ; Jong Han PARK ; Jong Guk KIM
Mycobiology 2015;43(3):231-238
A total of 4 aquatic plants, Eleocharis kuroguwai Ohwi, Hydrocharis dubia Backer, Salvinia natans All., and Zizania latifolia Turcz., were sampled from representative two wetlands of South Korea. A total of 38 endophytic fungal strains were isolated from aquatic plants native to the Daepyeong wetland, and 27 strains were isolated from the Jilnal wetland. The internal transcribed spacer regions of fungal isolates were sequenced and a phylogenetic analysis was performed. In addition, endophytic fungal diversity from each wetland and host plant species was deduced. A total of 25 fungal genera were purely isolated, and 16 fungal genera were isolated from each of the two wetlands. Commonly isolated genera from both wetlands were Aspergillus, Cladosporium, Clonostachys, Fusarium, Leptosphaeria, Penicillium, and Talaromyces. This study revealed that fungal diversity varied with environmental conditions and by host plant in representative two wetlands.
Aspergillus
;
Cladosporium
;
Eleocharis
;
Fresh Water
;
Fungi*
;
Fusarium
;
Korea*
;
Penicillium
;
Plants
;
Talaromyces
;
Wetlands*
6.Characterization of Paecilomyces variotii and Talaromyces amestolkiae in Korea Based on the Morphological Characteristics and Multigene Phylogenetic Analyses.
Thi Thuong Thuong NGUYEN ; Narayan Chandra PAUL ; Hyang Burm LEE
Mycobiology 2016;44(4):248-259
During fungal diversity surveys of the order Eurotiales in Korea, two fungal strains, EML-DG33-1 and EML-NCP50, were isolated from samples of rat dung and fig tree leaf collected at a garden located in Gwangju in 2014. To complete the National Species List of Korea, it is a prerequisite to verify whether many questionable species, which were previously recorded but not confirmed, indeed present in Korea. Herein, the isolates were confirmed as undescribed species, Paecilomyces variotii and Talaromyces amestolkiae based on the combination of morphological and phylogenetic analyses of multigenes including the rDNA internal transcribed spacer, β-tubulin, and RNA polymerase II subunit 2.
Agriculture
;
Animals
;
DNA, Ribosomal
;
Eurotiales
;
Gwangju
;
Korea*
;
Paecilomyces*
;
Rats
;
RNA Polymerase II
;
Talaromyces*
;
Trees
7.Descriptive Reports on Some Soil-Inhabiting Fungi in Korea.
Mycobiology 2001;29(2):90-95
During the study of microbial structures in root-regions of tomato and red pepper from fields, various soil-inhabiting fungi were isolated with the dilution plate technique. Among them an ascomycete, Emericellopsis mirabilis and three hyphomycetes, Gliocladium solani, Humicola veronae and Verticillium chlamydosporium are presented for the first time in Korea along with Talaromyces trachyspermus, Chaetomium globosum and Doratomyces microsporus.
Ascomycota
;
Capsicum
;
Chaetomium
;
Classification
;
Fungi*
;
Gliocladium
;
Korea*
;
Lycopersicon esculentum
;
Mirabilis
;
Mitosporic Fungi
;
Talaromyces
;
Verticillium
8.AcuD Gene Knockout Attenuates the Virulence of Talaromyces marneffei in a Zebrafish Model
Jiao FENG ; Zhiwen CHEN ; Liya HE ; Xing XIAO ; Chunmei CHEN ; Jieming CHU ; Eleftherios MYLONAKIS ; Liyan XI
Mycobiology 2019;47(2):207-216
Talaromyces marneffei is the only dimorphic species in its genus and causes a fatal systemic mycosis named talaromycosis. Our previous study indicated that knockdown of AcuD gene (encodes isocitrate lyase of glyoxylate bypass) of T. marneffei by RNA interference approach attenuated the virulence of T. marneffei, while the virulence of the AcuD knockout strains was not studied. In this study, T. marneffei-zebrafish infection model was successfully established through hindbrain microinjection with different amounts of T. marneffei yeast cells. After co-incubated at 28°C, the increasing T. marneffei inoculum doses result in greater larval mortality; and hyphae generation might be one virulence factor involved in T. marneffei-zebrafish infection. Moreover, the results demonstrated that the virulence of the ΔAcuD was significantly attenuated in this Zebrafish infection model.
Gene Knockout Techniques
;
Hyphae
;
Isocitrate Lyase
;
Microinjections
;
Mortality
;
Rhombencephalon
;
RNA Interference
;
Talaromyces
;
Virulence
;
Yeasts
;
Zebrafish
9.New Records of Four Species Belonging to Eurotiales from Soil and Freshwater in Korea
Monmi PANGGING ; Thuong TT NGUYEN ; Hyang Burm LEE
Mycobiology 2019;47(2):154-164
Four strains of Penicillium and Talaromyces species are described and illustrated in an inventory of fungal species belonging to Eurotiales. The strains, CNUFC-DDS17-1, CNUFC-DDS27-1, CNUFC-PTM72-1, and CNUFC-YJW3-31, were isolated from soil and freshwater samples from South Korea. Based on their morphological characteristics and sequence analyses by the combined β-tubulin and calmodulin gene, the CNUFC-DDS17-1, CNUFC-DDS27-1, CNUFC-PTM72-1, and CNUFC-YJW3-31 isolates were identified as Penicillium pasqualense, Penicillium sanguifluum, Talaromyces apiculatus, and Talaromyces liani, respectively. The designated strains were found to represent a previously undescribed species of Korean fungal biota. In this study, detailed morphological descriptions and phylogenetic relationships of these species are provided.
Biota
;
Calmodulin
;
Eurotiales
;
Fresh Water
;
Korea
;
Penicillium
;
Sequence Analysis
;
Soil
;
Talaromyces
10.Characterization and structure of a novel thermostable glucoamylase from Talaromyces leycettanus JCM12802.
Yujie GUO ; Tao TU ; Jin QIU ; Lige TONG ; Huiying LUO ; Bin YAO
Chinese Journal of Biotechnology 2019;35(4):616-625
Glucoamylase is a critical ingredient for saccharification in the starch decomposition, and widely used in food, pharmaceutical and fermentation industries. Glucoamylases are usually thermostable and have peak activities at high temperature, as required for the industrial process of glucose production. In this study, a glucoamylase gene belonging to the glycoside hydrolase (GH) family 15, Tlga15A, was cloned from Talaromyces leycettanus JCM12802, and successfully expressed in Pichia pastoris GS115. Recombinant glucoamylase TlGA showed optimal activities at pH 4.5 and 75 °C. The result of thermostability analysis showed that TlGA retained above 70% activity after incubating for 1 h at 65 °C, and 43% residual activity after 30 min at 70 °C. Moreover, TlGA had high resistance to most metal ions and chemical reagents tested. Various starch substrates could be hydrolyzed by TlGA, including soluble starch (255.6±15.3) U/mg, amylopectin (342.3±24.7) U/mg, glycogen (185.4±12.5) U/mg, dextrin (423.3±29.3) U/mg and pullulan (65.7±8.1) U/mg. The primary, secondary and tertiary structures of glucoamylase were further analyzed. The low ratio of Gly in the primary structure and low exposed nonpolarity solvent accessible surface in the tertiary structure may be the main reasons for TlGA's thermostability. These results show that TlGA is great promising for potential use in the commercial production of glucose syrups. Moreover, this research will provide knowledge and innovating ideas for the improvement of glucoamylase thermostability.
Cloning, Molecular
;
Enzyme Stability
;
Glucan 1,4-alpha-Glucosidase
;
Hydrogen-Ion Concentration
;
Pichia
;
Talaromyces
;
Temperature