1.Investigation on secondary metabolites of endophytic fungus Talaromyces purpurogenus hosted in Tylophora ovate.
Jing-Yi ZHAO ; Zhen LIU ; Sen-Feng SUN ; Yun-Bao LIU
China Journal of Chinese Materia Medica 2020;45(6):1368-1373
Eight compounds,(R)-2-[5-(methoxycarbonyl)-4-methyl-6-oxo-3,6-dihydro-2H-pyran-2-yl]acetic acid(1),(3S,4R)-3,4-dihydro-3,4-epoxy-5-hydroxynaphthalen-1(2H)-one(2),(-)-mitorubrinol(3),(-)-mitorubrin(4),(±)-asperlone A(5), terreusinone(6), verrucisidinol(7) and cerebroside C(8) were isolated from the endophytic fungus Talaromyces purpurogenus by using various column chromatographic techniques. Their structures were identified by NMR, MS, CD and optical rotation. Compounds 1 and 2 were new compounds. Their anti-diabetic activities in vitro were evaluated, and compound 1 showed moderate inhibitory activity toward XOD at 10 μmol·L~(-1) with the inhibition rate of 69.9%.
Endophytes/chemistry*
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Hypoglycemic Agents/chemistry*
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Magnetic Resonance Spectroscopy
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Molecular Structure
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Secondary Metabolism
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Talaromyces/chemistry*
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Tylophora/microbiology*
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Xanthine Oxidase/antagonists & inhibitors*
2.Two new polyesters from wetland soil-derived fungus Talaromyces flavus.
Jun-wei HE ; Hao GAO ; Xing-zhong LIU ; Xin-sheng YAO
China Journal of Chinese Materia Medica 2015;40(17):3347-3351
Two new polyesters, talapolyesters G-H (1-2) were isolated from the wetland soil-derived fungus Talaromyces flavus BYD07-13, and their structures were determined by NMR and MS spectroscopic data. The absolute configurations of the residues were determined by alkaline hydrolysis. The cytotoxicity against five tumor cell lines (HL-60, SMMC-7721, A-549, MCF-7 and SW480) of 1-2 was examined.
Cell Line, Tumor
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Cell Proliferation
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drug effects
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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pharmacology
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Humans
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Molecular Structure
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Polyesters
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chemistry
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isolation & purification
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pharmacology
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Talaromyces
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chemistry
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growth & development
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Wetlands
3.In vitro cytotoxicity and antimicrobial activity of Talaromyces flavus SP5 inhabited in the marine sediment of Southern Coast of India.
Bibin G ANAND ; C K Navin THOMAS ; S PRAKASH
Chinese Journal of Natural Medicines (English Ed.) 2016;14(12):913-921
Marine sediment samples were collected from the coastal areas of Southern India, particularly in Kanyakumari District. Twenty-eight different fungal strains were isolated. The screening of fungi from marine sediment was done to isolate a potent fungus that can produce bioactive compounds for biomedical applications. Only three strains viz Trichoderma gamsii SP4, Talaromyces flavus SP5 and Aspergillus oryzae SP6 were screened for further studies. The intracellular bioactive compounds were extracted using solvent extraction method. The crude extracts were tested for its anti-microbial and anti-cancer properties and analytically characterized using Gas Chromatography Mass Spectrometry (GC-MS). All the three extracts were active, but the extract from T. flavus SP5 was found to be more active against various human pathogens, viz., Pseudomonas aeruginosa ATCC 27853 (17.8 ± 0.1), Escherichia coli ATCC 52922 (18.3 ± 0.3), and Candida tropicalis ATCC 750 (17.7 ± 0.4). It also exhibited cytotoxic activity against HEp2 carcinoma cell line with the LC value of 25.7 μg·L. The GC-MS data revealed the presence of effective bioactive compounds. These results revealed that the extract from isolated fungus T. flavus SP5 acted as a potent antimicrobial, antifungal, and anticancer agent, providing basic information on the potency of marine fungi towards biomedical applications; further investigation may lead to the development of novel anticancer drugs.
Anti-Bacterial Agents
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chemistry
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metabolism
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pharmacology
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Antineoplastic Agents
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chemistry
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metabolism
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pharmacology
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Bacteria
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drug effects
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Cell Line, Tumor
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Fungi
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drug effects
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Gas Chromatography-Mass Spectrometry
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Geologic Sediments
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microbiology
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Humans
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India
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Microbial Sensitivity Tests
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Talaromyces
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chemistry
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genetics
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isolation & purification
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metabolism