1.Pseudolaric acid B induces human melanoma A375-S2 cell apoptosis in vitro.
Xian-Feng GONG ; Min-Wei WANG ; Shin-ichi TASHIRO ; Satoshi ONODERA ; Takashil IKEJIMA
China Journal of Chinese Materia Medica 2005;30(1):55-57
OBJECTIVETo study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells.
METHODMTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused.
RESULTPseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased.
CONCLUSIONThe major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.
Apoptosis ; drug effects ; Apoptosis Regulatory Proteins ; Cell Line, Tumor ; Diterpenes ; isolation & purification ; pharmacology ; Dose-Response Relationship, Drug ; Humans ; Melanoma ; metabolism ; pathology ; Pinaceae ; chemistry ; Proteins ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; bcl-2-Associated X Protein ; bcl-X Protein
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