Objective: This study intended to clarify whether healthcare professionals provide palliative care and the factors associated with such care. Methods: An anonymous self-administered questionnaire survey was conducted of healthcare professionals in in an acute care hospital in order to investigate their practice and understanding of palliative care as well as their personal attributes. A multivariate logistic regression analysis was conducted to identify factors associated with their palliative care practice. Results: 605 of 955 respondents (response rate: 63%) answered. Twenty-three percent of all respondents answered that they were involved in palliative care practice. A multivariate logistic regression analysis revealed understanding the concept and practical components of palliative care, including the functions of palliative care, differences between primary and specialized palliative care, and advance care planning, were factors associated with palliative care practice. Conclusion: Palliative care specialists should provide the educational support for healthcare professionals to enable them to deepen their understanding of palliative care. Such support from the palliative care specialist may promote the health care professionals’ awareness of their own roles in palliative care.