1.Clinical application of ballon-assisted surgical wrapping-clipping for blood blister-like aneurysms of internal carotid artery
Lin HE ; Hong CAI ; Chunqin ZHAO ; Zhong CHEN ; Taishan CHEN ; Yi LIU
Chinese Journal of Postgraduates of Medicine 2017;40(8):728-731
Objective To investigate the effectiveness and safety of ballon-assisted surgical wrapping-clipping for the treatment of blood blister-like aneurysms (BBA) of internal carotid artery. Methods The clinical data of 9 patients with BBA of internal carotid artery who were treated by ballon-assisted surgical wrapping-clipping from August 2011 to June 2016 were retrospectively analyzed. Results All 9 patients were successfully treated with surgical wrapping-clipping in ballon-assisted technique. The BBA had no rebleeding.Nine cases were followed up clinically for six months. Digital subtractive angiography demonstrated no aneurysm in these patients, and showed no stenosis in the parent artery. Conclusions Ballon-assisted surgical wrapping-clipping in the treatment of BBA is effective and safety. It may help to reduce the risks of BBA rebleeding and recurrence.
2. Nursing intervention for respiratory function exercise in patients with silicosis complicated by stable chronic obstructive pulmonary disease
Xin WANG ; Hong JIANG ; Haitao YU ; Fang JU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2018;36(11):844-846
Objective:
To investigate the clinical effect of nursing intervention for respiratory function exercise in patients with silicosis complicated by stable chronic obstructive pulmonary disease (COPD) .
Methods:
A total of 60 patients with silicosis complicated by stable COPD who were hospitalized in Department of Occupational Diseases, Laigang Hospital Affiliated to Taishan Medical College, from August 2017 to April 2018 were enrolled and randomly divided into control group and observation group, with 30 patients in each group. The patients in the control group were given routine treatment and respiratory function exercise, and those in the observation group were given nursing intervention in addition to the treatment in the control group. The two groups were compared in terms of pulmonary function, blood gas parameters, and six-minute walk distance before intervention and after 2 months of intervention.
Results:
After intervention, the observation group had significantly higher forced expiratory volume in 1 second (FEV1) , forced vital capacity (FVC) , arterial partial pressure of oxygen (PaO2) , and six-minute walk distance than the control group (
3.Retrospective analysis of 202 pathological autopsy cases in medical dispute.
Yihu FANG ; Keli ZHANG ; Haisheng YU ; Xuan LI ; Taiping ZHENG ; Taishan HONG ; Liu CAO
Journal of Zhejiang University. Medical sciences 2013;42(4):456-460
OBJECTIVETo analyze the characteristics of autopsies in medical dispute.
METHODSThe data of 202 autopsy cases in medical disputes performed by the Department of Pathology of Jiangxi Medical College from January 2001 to December 2010 were retrospectively analyzed.
RESULTSThe number of autopsy in medical disputes increased year by year. Neonatal, infant and 30 ≊ 60y were more common age groups in dispute; the gender ratio of male to female was 2:1 and more cases were from hospitals in rural areas. Most medical disputes came from in departments of pediatric, obstetrics and gynecology and general surgery. Death caused by cardiovascular diseases ranked at the first place.
CONCLUSIONAutopsy is important in medical disputes to define the cause of death and to preserve evidence.
Adolescent ; Adult ; Aged ; Autopsy ; statistics & numerical data ; Cardiovascular Diseases ; mortality ; Cause of Death ; Child ; Child, Preschool ; Dissent and Disputes ; Female ; Hospital Departments ; statistics & numerical data ; Humans ; Infant ; Infant, Newborn ; Male ; Malpractice ; Middle Aged ; Retrospective Studies ; Sex Ratio ; Young Adult
4.Effect of different treatments of dentin surface on sheer bond strength between different bonding agents and dentin.
Xiao-yan HAN ; Hong-shui ZHU ; Qiu-yue LIU
West China Journal of Stomatology 2008;26(2):125-128
OBJECTIVETo evaluate the effect of Zinc oxide-eugenol cement and Gluma desensitizer on the sheer bond strength of three kinds of dentin bonding agents. The three dentin bonding agents were Zinc phosphate cement, Glass ionomer cement and Super-Bond C&B. To find the theory depending for the using of different protective methods and the selecting of different kinds of dentin bonding agents in prepared abutment teeth.
METHODSThe buccal surfaces of ninety freshly extracted human premolars were flattened to expose an adequate area of lower dentin. Followed by wet-grinding on a series of silicon carbide paper from number 320, 400, 600 grit to produce the dentin bonding surface. The teeth roots were embedded in self-curing resin with the crown out of the resin. The embedded ninety teeth were divided randomly into three groups. The group A was control and the dentin surfaces were not treated. The group B was covered with a paste of Zinc oxide-eugenol cement. The group C was covered with Gluma desensitizer. Calculating the sheer strength between three bonding agents and dentin after the two treatments of dentin surface. The results were statistically assessed with SPSS software. Dentin surfaces were observed with scanning electron microscope (SEM).
RESULTSThe sheer bond strengths of Zinc phosphate cement had significant decrease (P<0.05), especially the C1 group. The sheer bond strengths of Glass ionomer cement and Super-Bond C&B had no significant difference.
CONCLUSIONZinc oxide-eugenol cement and Gluma desensitizer could reduce the sheer bond strength of Zinc phosphate cement with dentin surface. Zinc oxide-eugenol cement and the Gluma desensitizer could not effect Glass ionomer cement and the Super-Bond C&B with dentin.
Boron Compounds ; Crowns ; Dental Bonding ; Dentin ; Dentin-Bonding Agents ; Glutaral ; Humans ; Methacrylates ; Methylmethacrylates ; Resin Cements ; Zinc Oxide-Eugenol Cement
5.Effect of nitric oxide on the expression of apelin receptor mRNA in rat caudate nucleus.
Bo BAI ; Yu-Hong LIU ; Hai-Qing LIU
Neuroscience Bulletin 2007;23(3):180-184
OBJECTIVETo investigate the effect of nitric oxide (NO) on the expression of apelin receptor mRNA, as well as their correlation, in the caudate nucleus of rat.
METHODSL-Arginine (L-Arg), N(G)-nitro-L-arginine methyl ester (L-NAME) and normal saline (NS) was separately microinjected into rat caudate nucleus. Expressions of neuronal NO synthase (nNOS) mRNA and apelin receptor mRNA were detected by RT-PCR at 4, 8, 12, 24 and 48 h after microinjection, and their correlation was determined.
RESULTSThe expressions of nNOS mRNA and apelin receptor mRNA were both significantly increased after microinjection of L-Arg, but significantly decreased after microinjection of L-NAME compared with the NS control group. The nNOS mRNA had a positive correlation with the expression of apelin receptor mRNA after microinjection of L-Arg and L-NAME.
CONCLUSIONThe activity of NOS in the central nervous system, especially in the caudate nucleus, is one of the key factors for NO to exert many kinds of biological actions, such as modulation of central pain, as a neurotransmitter. The neurobiological action of NO in rat caudate nucleus may be associated with apelin receptors.
Animals ; Apelin Receptors ; Arginine ; pharmacology ; Caudate Nucleus ; drug effects ; metabolism ; Enzyme Inhibitors ; pharmacology ; Gene Expression Regulation ; drug effects ; physiology ; Male ; NG-Nitroarginine Methyl Ester ; pharmacology ; Nitric Oxide ; physiology ; Nitric Oxide Synthase Type I ; metabolism ; RNA, Messenger ; Rats ; Rats, Wistar ; Receptors, G-Protein-Coupled ; genetics ; metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; methods
6. Expression of the VP1 protein and establishment of indirect ELISA detection method of Coxsackievirus B4
Weiwei ZHANG ; Hong ZHOU ; Juan LI ; Zhenjie ZHANG ; Weifeng SHI
Chinese Journal of Experimental and Clinical Virology 2019;33(3):303-308
Objective:
To detect antibodies to Coxsackievirus B4 (CV-B4), the indirect enzyme-linked immunosorbent assay (ELISA) method was established and optimized using the recombinant VP1 protein expressed in the prokaryote system as the envelope antigen.
Methods:
The VP1 gene of CV-B4 was amplified using reverse transcriptase-polymerase chain reaction (RT-PCR). It was ligated into the expression vector pET32a(+ ) to obtain the recombinant plasmid pET32(+ )-VP1 and was then transformed into
7.A clinical analysis of spontaneous bacterial peritonitis in patients with severe hepatitis and hepatic cirrhosis.
Yu-jiang ZHANG ; Shi-ling ZHANG ; Qiao-lin WANG ; Hong-feng YAN ; Yong-xing TAN
Chinese Journal of Hepatology 2003;11(7):441-441
Aged
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Bacterial Infections
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drug therapy
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etiology
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Female
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Hepatitis, Chronic
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complications
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Humans
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Liver Cirrhosis
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complications
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Male
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Middle Aged
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Peritonitis
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drug therapy
;
etiology
8.Preventive effects of cerebro cellular growth peptide on gentamycin-induced inner ear damage in guinea pigs.
Song-Jian KANG ; Xian-Jun SHI ; You-Zhen WEI ; An HONG ; Xin-Quan JIANG
Chinese Journal of Applied Physiology 2003;19(1):90-93
AIMTo investigate the preventive effects of the cerebro cellular growth peptide (CCGP) on gentamycin-induced inner ear damage in guinea pigs, and to clarify its mechanism.
METHODSThe hypoacusis severity and enzymatic activity in the cochlear hair cells were examined by brainstem auditory evoked potential (BAEP) and histochemistry, respectively. The damaged hair cells was counted in three groups.
RESULTSCCGP reduced the elevated BAEP reaction thresholds. It protected activities of mitochondrial succinate dehydrogenase and lysosome acid phosphatase in the cochlear hair cells. The number of damaged hair cells in the CCGP group was less than that in the gentamycin (GM) group.
CONCLUSIONCCGP can reduce GM ototoxicity. The mechanism may be associated with the protective activity of mitochondrial enzyme, the maintenance of lysosome intactness, energy metabolism of the cochlear hair cells, and reduction of autolysis of hair cells induced by hydrolase over flowing from lysosome.
Animals ; Evoked Potentials, Auditory, Brain Stem ; Female ; Gentamicins ; toxicity ; Guinea Pigs ; Hair Cells, Auditory ; drug effects ; physiology ; Male ; Nerve Growth Factor ; pharmacology
9.Inhibitory effect of caveolin-1 on endoplasmic reticulum stress-induced apoptosis in macrophages via p38 MAPK pathway.
Wen YUE ; Shu-Tong YAO ; Xiao ZHOU ; Yan-Hong SI ; Hui SANG ; Jia-Fu WANG ; Zhan-Ping SHANG
Acta Physiologica Sinica 2012;64(2):149-154
Endoplasmic reticulum (ER) stress occurs in macrophage-rich areas of advanced atherosclerotic lesions and contributes to macrophage apoptosis and subsequent plaque necrosis. The purpose of the present study was to investigate the effects of caveolin-1 (Cav-1) on ER stress-induced apoptosis in cultured macrophages and the underlying mechanisms. RAW264.7 cells were incubated with thapsigargin (TG) to establish ER stress model. And Cav-1 expression was detected by Western blot. After being pretreated with filipin(III), a caveolae inhibitor, RAW264.7 cells were assayed with flow cytometry and confocal laser scanning microscopy to detect cell apoptosis. Moreover, p38 mitogen-activated protein kinase (MAPK) phosphorylation and C/EBP homologous protein (CHOP) expression were detected with Western blot. The results showed that Cav-1 expression was markedly increased at early stage of TG treatment (P < 0.05) and then decreased with prolonged or high dose TG treatments. The increasing of Cav-1 expression induced by TG in RAW264.7 cells was abolished under inhibition of caveolae by filipin(III) (P < 0.05). The effect of TG on apoptosis of RAW264.7 cells was further augmented after pretreatment with filipin(III) (P < 0.05). Western blotting showed that MAPK phosphorylation induced by TG was inhibited by filipin(III) in RAW264.7 cells (P < 0.05), whereas CHOP remained unchanged (P > 0.05). These results suggest that Cav-1 may play a critical role in suppressing ER stress-induced macrophages apoptosis in vitro, and one of the mechanisms may be correlated with the activation of p38 MAPK prosurvival pathway.
Animals
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Apoptosis
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drug effects
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Caveolin 1
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genetics
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metabolism
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Cell Line
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Endoplasmic Reticulum Stress
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physiology
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Filipin
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pharmacology
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MAP Kinase Signaling System
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Macrophages
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cytology
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drug effects
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Mice
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Thapsigargin
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pharmacology
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Transcription Factor CHOP
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metabolism
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p38 Mitogen-Activated Protein Kinases
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metabolism