Phosphatase of regenerating liver-3 (PRL-3) is a novel small molecule protein-tyrosine-phosphatase,which plays an important role in the oncogenesis and deveopment of tumors.Studies show that PRL-3 regulates neoplasm progress through participating in multiple signal pathways.Its high expression can significantly promote neoplasm metastasis in cancer tissue.However,there is no expression of PRL-3 in most normal tissue.PRL-3 is expected to be a potential tumor maker of diagnosis and a new target for the therapy of cancer.

Luminol dependent chemilumi-nescence (CL) method was used to observe effect of nifedipine on polymorphonuclear leukocytes (PMN) oxygen metabolism in coronary heart disease (CHD). Studied subjects including 24 cases of coronary heart disease non using nifedipine, 26 cases of coronary heart disease using nifedipine group (10.mg tid on 3~7 days), 30 cases of normal drug-free subjects.The results showed that: 1. PMN-CL in using nifedipine CHD group was sigrlifficantly lower, its PMN- CL backgroud, peak value andphargocyte index was signifficantly lower than that in non using nifedipine CHD group. 2. PMN-CL peak value and phargocyte index in using nifedipine CHD group had no signifficantly differences compared with normal group. 3. Parameters of PMN- CL in non using nifedipine CHD group were signifficantly higher than that in normal group.

Pancreatic cancer is an aggressive malignant gastrointestinal tumor,surgical resection offers the only chance of cure. Due to its anatomic and biological characters,early stage pancreatic cancer is usually clinically silent,80％-85％ of patients present with advanced unresectable disease. Furthermore,pancreatic cancer responds poorly to most radiochemotherapeutic agents.In this article,we discuss some clinical aspects including early diagnosis,preoperative systemic assessment,surgery and perioperartive adjuvant therapy,and we hope to find the challenges as well as strategies for pancreatic cancer in order to further improve the current level of diagnosis and treatment in China.

Objective　To explore left atrial(LA) structur e and function changes in 86 cases of essential hypertension disease (EH) and ef fects of age,gender,hypertension duration,hypertension stage and left ventricula r function on them.Methods　 Using M-mode echocardiogram to detect LA diameter, Usi ng ultrasonic acoustic quantification (AQ) to detect LA area,LA systolic and dia stolic function,LV systolic and diastolic function. Results　①Compared with normal group, LA diameter and LA area we re larger in EH group, LA systolic and diastolic function were impaired. ② Gend er had no effect on LA structure and functione in EH patients; LA diameter and L A area dilated with age, LA systolic function was impaired with age. ③ Hyperten sion duration had no effect on LA structure and function in EH patients. ④LA d iameter and area dilated with hypertension stage. With the development of hypert ension, LA diastolic function was first impaired,then LA systolic function was a lso impaired.⑤LA structure and function had signifficant relationship with LV d iastolic function, LA might dilate and LA systolic function might be impaired wi th LV diastolic function damage.Conclusions　LA diameter and LA area are dilated and LA function i s impaired in EH patients, which changes become more signifficant with age. LA s tructure change and systolic function impairment have a relationship with LV dia stolic dysfunction.

Objective: A HPLC method for determination of emodin and rhein in Niuhuangjiedu Tablets was established. Methods: The chromatographic conditions were listed below: Diamonisil ODS C 18 column, UV detector at 254nm of wavelength, a mobile phase of methanol water phosphate acid(720∶280∶1),and 1mL?min -1 of flow rate. The retention time of emodin was 8.49 min and that of rhein was 15.56min. Results: The calibration curves were linear for emodin within 0.469～1.407?g( r =0.9992) and for rhein within 0.3125～ 0.9375?g ( r = 0.9992). The recoveries were 98.2%( RSD =0.43%)for emodin and 98.4%( RSD =0.72%)for rhein, respectively. Conclusion: This method is simple and the separate result is satisfactory.

ment with simple operation, high successful rate, high safety, less invasiveness and low complication rate.

Objective To evaluate the role of polymorphonuclear (PMN) in patients with acute myocardial infarction (AMI).The preventive effects of Vitamin C-a potent free radical scavenger,on parameters of PMN oxygen free radicals (OFR) production in patients with AMI were also studied.Methods 60 patients with AMI were randomized to receive either conventional treatment only (group Ⅰ,n=30) or conventional treatment supplemented with vitamin C infusion,3.0 g/d,for 7 days (group Ⅱ,n=30).Parameters of PMN OFR production were assayed by a method named as polymorphonuclear chemiluminescence (PMN-CL) on the 1st,3rd,7th and 10th day after been hospitalized.62 healthy controls of similar age,sex were also studied.Results Parameters of PMN-CL increased significantly in patients with AMI compared with that in the healthy controls.Parameters of PMN-CL decreased significantly in group Ⅱ on the 3rd,7th and 10th day compared with that in group Ⅰ(P<0.05,0.01,0.001,respectively).ST segment score was significantly higher in group Ⅱ than that in group Ⅰ on the 3rd,7th and 10th day.Conclusion These results indicate that the PMN act as a potential contributor to extension of tissue injury induced by OFR.Supplementation with vitamin C may suppress PMN OFR production and could be beneficial in preventing myocardial necrosis.

Objective To evaluate the clinical value of speckle tracking imaging in the measurement of two-dimensional strain in patients with myocardial infarction. Methods High frame rate two-dimensional echocardiography were performed in 30 healthy subjects,21patients with anterior myocardial infarction and 15 patients with inferior myocardial infarction. Longitudinal strain (LS), radial velocity (Vs), regional shortening fraction (RFS),radial strain (RS) were measured in the apical two-chamber view and short-axis view(the level of papillary muscle) using two-dimensional strain software. Results Compared with healthy subjects, LS, Vs, RFS, RS decreased in the segments with infarction(P<0.05). LS, Vs, RFS, RS of the two myocardial infarction groups had partly differences(P<0.05). Conclusions Regional wall motion of left ventricle in patients with myocardial infarction can be evaluated accurately with speckle tracking imaging.

Objective:To investigate the immunohistochemical characterization of hepatic stem cells in the developing human liver, so as to study the origin, differentiation and migration of hepatic stem cells. Methods: H-E staining and immunohistochemical methods were used to observe the expression of hepatic/cholangiocellular differentiation markers (AFP,GST-?,CK7,CK19) and hematopoietic stem cell markers (CD34 and c-kit) in several kinds of cells obtained from thirty 4-to 35-week old fetal liver samples. Results: AFP expression appeared in fetal liver at 4 weeks’ gestation,peaked during 16-24 weeks’ gestation and decreased gradually afterwards; finally weak signals were only found in some ductal plate cells and a few limiting plate cells. GST-? was detected in hepatic cord cells from the 6th week and in the ductal plate cells from the 8th week; 26 weeks later, only some ductal plate cells and a few limiting plate cells showed positive signals. CK19 expression peaked during 6th to 11th week in hepatic cord cells and decreased gradually afterwards, except for that in the ductal plates. CK7 expression was limited in the ductal plate cells and bile duct cells from the 14th week. CD34 and c-kit were detected at the 8th week in some ductal plate cells and a few mononuclear cells in the hepatic cords/mesenchymal tissue of portal area; after 21 weeks, CD34 and c-kit were found only in ductal plate cells and a few mononuclear cells in the hepatic mesenchymal tissue of portal areas. Conclusion: Fetal hepatocytes at 4-16 weeks’ gestation are mainly constituted by hepatic stem cells with bi-potential differentiation capacity. At 16 weeks’ gestation, most hepatic cord cells begin to differentiate into hepatocytes and abundant hepatic stem cells remain in the ductal plate (the origin site of Hering canals). It is also indicated that the hematopoietic stem cells may give rise to some hepatic stem cells in embryonic liver. These indirectly support the hypothesis about the location and origin of liver stem cells in “liver valley hypothesis” reported previously.

Objective To discuss the methods and clinical effects of phased treatment of ankylosing spondylitis (AS) combined with severe hip flexion contracture.Methods The study enrolled 8 cases (12 hips) of AS combined with severe hip flexion contracture hospitalized from September 2011 to November 2012.Phased treatments included lateral hip arthrolysis,articular capsulectomy,stripping of the reflected head of rectus femoris,femoral neck osteotomy,traction and stage Ⅱ biotype total hip arthroplasty (THA).Preoperative and postoperative Harris score,hip range of motion,and complication of femoral nerve injury were detected.Results Period of follow-up was 6-12 months (mean 10 months).One case developed heterotopic ossification,which affected postoperative hip activity and received resection one year later.One case sustained fissure fracture of calcar femorale during implantation of the prosthetic femoral stem,but no special handling was provided.Of all cases,active flexion and extension of the hip were both 0° before operation,but increased to (96.25 ± 4.33) ° and (24.17 ± 4.69)° respectively after operation ; mean Harris score was improved from (26.67 ± 2.39) points preoperatively to (90.92 ± 5.66) points postoperatively (P ＜ 0.01).Besides,no femoral nerve injury was observed.Conclusion Phased treatment of AS combined with severe hip flexion contracture restores hip function and minimizes femoral nerve injury following THA.