Objective To establish an high performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method for determination of glucosamine hydrochloride and its related substances in glucosamine and indometacin enteric-coated capsules.Methods Separation was carried out on a Inertsil C8 Column (250 mm ×4.6 mm,5μm) with a mobile phase composed of acetonitrile-water(70∶30) at a flow rate of 1.0 mL/min and the column temperature was 25℃.Results The linear ranges of calibration curve was 25 -312.5 μg/mL(r=0.9991, n=6).The average recovery was 98.0%, and the relative standard deviation was 1.3%( n =5 ) .The glucosamine hydrochloride was stable with acid destruction, but unstable with alkali, heat and strong light destruction.Conclusion The method is simple, accurate and reproducible for determination of glucosamine hydrochloride and related substances.

Objective To develop a new high performance liquid chromatography (HPLC ) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of two indicative components (peimine and peimi-nine) in Xiaoer Baotaikang granules .Methods The analysis was performed on a Thermo C18 column (250 mm × 4 .6 mm , 5 μm) by using a mobile phase consisted of acetonitrile-water and diethylamine (65 ∶ 35 ∶ 0 .03) ,and the flow rate was 1 .0 ml/min;ELSD parameters :the temperature of drift tube was 85 ℃ and the carrier gas flow rate was 2 .2 L/min .Results The linear ranges of peimine and peiminine were 0 .586~5 .860 μg (r=0 .999 6) and 0 .564~5 .640 μg (r=0 .999 9) ,respec-tively .The average recovery rate for peimine and peiminine was 99 .44% (RSD was 0 .7% ) and 98 .56% (RSD was 1 .0% ) , respectively .Conclusions The method is sample ,accurate and reproducible ,which could be used for the quality control of Xiao-er Bao taikang g ranules .

Objective To explore the short-term efficacy of individualized motion control training for the patients with physiological sacroiliac joint dysfunction. Methods 58 patients were diagnosed as sacroiliac joint dysfunction by standing and sitting stoop test and single leg test. All the patients were randomly divided into two groups. Rotation of the iliac bone was adjusted by muscle energy technique and impact technique in all the patients. The study group received additional exercise control training including local muscle drafting,flexibility and stability. The short-term efficacy was observed. Results VAS and ODI in the two groups were improved significantly after treatment,and there was significant difference between the two groups before treatment(P < 0.01). Three weeks after treatment,the VAS score and ODI in the control group increased,while those in the study group did not change significantly. There was a significant difference in the continuous efficacy of treatment between the two groups(P < 0.05). Conclusions Motion control training based on manipulative exercise test evaluation has a better continuous efficacy for patients with no-specific low back pain and sacroiliac joint dysfunction. It is worth clinically popularizing.