1.A clinical observation of heart rate turbulence of diabetes
Chinese Journal of Diabetes 2009;17(12):933-934
Objective To discuss the significance and alteration of heart rate turbulence (HRT) of diabetics with ventricular premature beats of coronary heart disease (CHD). Methods 107 patients were chosen and divided into DM group(n=30), CHD group (n=32)and normal group(n=45).The turbulence onset(TO)and turbulence slope(TS) were calculated and analyzed. Results For the diabetics with ventricular premature beats, TO value was higher and TS value was lower than the normal group(all P<0.05).The value of TO and TS of the diabetics with ventricular premature beats were similar to those of CHD(P>0.05). Conclusions HRT can be used to estimate the integrality and stability of cardiac autonomic nerve function for the diabetics and it is an important factor for diagnosis.
2.Misdiagnosis analysis for 74 cases of acute abdominal pain as the first symptom of non-digestive diseases
Chinese Journal of Primary Medicine and Pharmacy 2010;17(15):2026-2027
Objective To explore the abdominal pain as initial symptom of non-digestive diseases,the causes of misdiagnosis. Methods 74 patients with clear diagnosis non-digestive diseases which initial symptom was acute abdominal pain were analyzed. Results The most common causes of misdiagnosis were blood system diseases,circulatory diseases,metabolic diseases,endocrine system diseases,respiratory diseases,nervous system disease,accounting for 35.1% ,25.7% ,10. 8% ,8. 1% ,6. 8% ,6. 8%.The rest,such as the urinary system diseases,toxic diseases were not rare. Conclusion Non-digestive diseases which initial symptom was acute abdominal pain could be caused by the body's various systems,and the causes were complex,the diseases conditions were critical and changed rapidly,so it was difficult to diagnose and had to be Paid attention on.
3.Clinical value of electrocardiogram changes on the diagnosis of hyperkalemia
Feng TIAN ; Taike ZHANG ; Li ZHAO ; Yunling QI ; Yuanyuan WANG
Chinese Journal of Primary Medicine and Pharmacy 2008;15(10):1635-1636
Objective To research clinical significance of electrocardiogram(ECG) measuring in diagnosis of hyperkalemia. Methods ECG changes and serum potassium concentration from 72 cases have been analyzed and contrasted. Results Forty-one cases with hyperkalemia are detected by ECG changes. Coincidence rate is up to 56.9% with serum potassium test. When the serum potassium concentration exceeds 5.5mmol/L, the accuracy of ECG in diagnosis of hyperkalemia was 22.2%. The main features arc peaked T wave with tabernacle shape ;when the serum potassium concentration exceeds 6.7mmol/L, the accuracy of ECG in diagnosis of hyperkalemia was 74.3%. The main features are ST debased,P wave with low amplitude and time limit increased. Some patients appeared sinus node-ventride conduction. One or two appeared malignant ventricular arrbythmia; when the serum potassium concentration exceeds 10.0mmol/L,the accuracy of ECG was 100% ,most patients appeared malignant ventricular. Conclusion The electrocardiogram measurement has the better consistency with serum potassium test, and abnormal electrocardio-gram will be eccurred,when mixed other electrolyte unbalance. Pseudo-hyperkalemia can be distinguished accurately and conveniently by using this method.
4. Changes of the expression for genes related with senescence and the telomerase activity during cellular replicative and premature senescence in human embryonic lung fibroblasts
Jianping YANG ; Wenjuan ZHANG ; Chunxia JING ; Chipeng WU ; Weidong JI ; Linqing YANG ; Zhixiong ZHUANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2017;35(1):15-18
Objective:
To detect the alterations of telomerase activity and the expression for oxidative stress responsive genes related with senescence during cellular replicative senescence and hydrogen peroxide-induced premature senescence in human embryonic lung fibroblasts (HELFs) in vitro.
Methods:
The HELFs were divided into young cells (22 population doubling levels, 22PDL) , mid-aged cells (35PDL) and replicative senes-cent cells (49PDL) and premature senescent cells induced by H2O2(premature senescence, PS). The telomerase activity was detected by ELISA assay during cellular replicative and premature senescence. The mRNA level of oxidative stress responsive genes related with senescence for Foxo1, Foxo3, Pdx1, apoA-I and MMP1 was per-formed by RT-Q-PCR separately.
Results:
The mRNA level for Foxo1, Foxo3, apoA-I and Pdx1 was decreased separately during cellular replicative senescence compared to that in the young-stage cells with statistical signifi-cance (