Objective To investigate the protective mechanism of Salvianolic acid B (Sal B) on experimental in-vivo myocardial ischemia-reperfusion injury (MIRI)in rats. Methods Rats model of myocardial ischemia-reperfusion injury was induced by ischemia for 60 min and then reperfusion for 60 min. After treatment,endothelin (ET) levels in plasma and the homogenate of myocardial tissue,and serum levels of nitric oxide (NO) and NO synthase were measured. Meanwhile,the pathological changes of myocardial tissue were also observed. Results Compared with the model group,middle-dose and high-dose Sal B could depress the ET levels in the plasma and the myocardial tissue,and increase the content of serum NO and NOS (P

Objective To observe the protective effect of total flavonoids from hawthorn leaf on ligation-induced acute myocardial ischemia in anesthetized dogs. Methods The coronary arteries of anesthetized dogs were ligated to induce the model of acute myocardial ischemia. The indexes including the changes of ST segment in electrocardiogram (∑-ST and N-ST) and the release of LDH and CK were measured after treatment. Meanwhile,the size of myocardial ischemia was observed by TTC staining method. Result Total flavonoids from hawthorn leaf depressed the increase of ∑-ST and N-ST,decreased the release of LDH and CK,and reduced the myocardial ischemic size. Conclusion Total flavonoids from hawthorn leaf has an effect on relieving myocardial ischemia.

OBJECTIVETo identify and elucidate the vasorelaxant activity of homoisoflavonoids, the main chemical components from Lignum Sappan (the stems of Caesalpinia sappan), in isolated rat thoracic aortic rings pre-contracted with phenylephrine (PE, 1 micromol x L(-1)) and KCl (60 mmol x L(-1)).

METHODThe tension of rat thoracic aorta rings was used to evaluated the vasorelaxant activities of four homoisoflavonoids, brazlin (1), (E)-3-(3,4-dihydroxybenzylidene)-7-hydroxychroman-4-one (2), sappanone B (3), 3-deoxysappanone B (4).

RESULTCumulative addition of homoisoflavonoids (2, 3 and 4) (50-1000 micromol x L(-1)) exhibited an acute relaxation either in endothelium-intact or endothelium-denuded rings in a concentration-dependent manner. However, this relaxation was significantly inhibited in endothelium-denuded condition and in the presence of endothelial nitric oxide synthase (eNOS) inhibitor, N(W)-nitro-L-arginine methyl ester (L-NNA, 100 micromol x L(-1)), and a soluble guanylate cylcase (sGC) inhibitor, methylene blue (MB, 10 micromol x L(-1)) when addition of variation homoisoflavonoids brazlin (1) (50-1000 micromol x L(-1)).

CONCLUSIONThese results indicate that normo-homoisoflavonoids (2, 3 and 4) from Caesalpinia sappan mediates endothelium-independent vasodilator action in rat thoracic aortic rings, while the variation homoisoflavonoids brazlin elicits endothelium-dependent relaxation might via nitric oxide (NO)-cGMP pathway. This research could explain the pharmacological activities of homoisoflavonoids to a certain degree.

Animals ; Aorta, Thoracic ; drug effects ; physiology ; Caesalpinia ; chemistry ; Endothelium ; metabolism ; Enzyme Inhibitors ; pharmacology ; Flavonoids ; chemistry ; pharmacology ; Guanylate Cyclase ; antagonists & inhibitors ; Male ; Muscle Contraction ; drug effects ; NG-Nitroarginine Methyl Ester ; pharmacology ; Nitric Oxide Synthase Type III ; antagonists & inhibitors ; Phenylephrine ; pharmacology ; Potassium Chloride ; pharmacology ; Rats ; Receptors, Cytoplasmic and Nuclear ; antagonists & inhibitors ; Soluble Guanylyl Cyclase ; Vasodilation ; drug effects ; Vasodilator Agents ; chemistry ; pharmacology

Taking into consideration the characteristics and current development status of air medical rescue,an analysis is conducted from the perspective of hospitals to examine the focal points and challenges in establishing the capability of hospital air medical rescue.A capability framework comprising five modules,namely planning system,service model,professional teams,hardware platform,and operational procedures,is proposed.The key tasks for each module are sorted out.Furthermore,the ex-ploration and practical experience of The First Affiliated Hospital of Sun Yat-sen University are shared,with the aim of providing a reference for the construction of hospital air medical rescue capabilities in the modern era.