Objective:To explore the value of IL-6 in the diagnosis, treatment and improving prognosis of urosepsis at 2 hours after percutaneous nephrolithotomy.Methods:Twenty-five patients with urosepsis undergoing percutaneous nephrolithotomy in the Shandong Provincial Hospital Affiliated to Shandong First Medical University from January 2019 to June 2021 were analyzed retrospectively, including 6 males and 19 females, aged (55.4±11.6) years, and there were 10 cases with hypertension, 11 cases with diabetes and 14 cases with hydronephrosis. There were 10 cases with Stone volume>353mm 2, 22 cases with pyuria, 12 cases with positive nitrite and 18 cases with positive urine culture before operation. Among them, 13 patients were classified into experimental group with the test of IL-6 at 2 hours after operation and 12 patients were classified into control group without the test of IL-6 at 2 hours after operation. IL-6>1 000 pg/ml at 2 hours after operation as the standard for diagnosis of urosepsis was used to guide the anti-infective treatment after percutaneous nephrolithotomy. The operation time, severity of urinary sepsis (severe sepsis and septic shock), diagnostic time of urosepsis after operation, and hospital stay after operation were compared between the two groups. Results:There were 13 patients including 9 females with average age of (52.4±12.2) years in the experimental group, and there were 6 cases with hypertension, 5 cases with diabetes and 9 cases with hydronephrosis, 5 cases with stone volume>353mm 2, 12 cases with pyuria, 7 cases with positive nitrite and 10 cases with positive urine culture before operation. There were 12 patients including 10 females with average age of (58.7±10.5) years in the control group, and there were 4 cases with hypertension, 6 cases with diabetes, 5 cases with hydronephrosis, 3 cases with stone volume>353mm 2, 10 cases with pyuria, 5 cases with positive nitrite, and 8 cases with positive urine culture before operation. There was no significant difference between the two groups in terms of gender( P=0.645), average age ( P=0.182), hypertension( P=0.688), diabetes( P =0.695), hydronephrosis( P=0.238), stone volume>353 mm 2( P=0.673), pyuria( P=0.593), positive nitrite( P=0.695), and positive urine culture( P=0.673). There was not significant difference in the general clinical data of gender, age, hypertension, diabetes, stone burden, hydronephrosis, pyuria, positive nitrite and positive urine culture between the two groups ( P>0.05). The mean value of IL-6 in the test group was (6 824.4±1 473.3) pg/ml. The operative time of the experimental group with (100.8±27.8) minutes was longer than that of the control group with (88.3±39.3) minutes, but the difference between the two groups was not statistically significant ( P=0.645). The diagnostic time of urosepsis after operation in the test group with (3.6±2.0) hours was shorter than that in the control group with (6.0±3.0) hours, and the difference between the two groups was statistically significant ( P=0.023). The postoperative hospital stay in the test group with (6.1±1.6) days was shorter than that in the control group with (8.2±2.3) days, and the difference between the two groups was statistically significant ( P=0.014). Three patients with severe sepsis and septic shock in the test group, which were less than that of eight patients with severe sepsis and septic shock in the control group, and the difference between the two groups was statistically significant ( P=0.047). Conclusion:The detection of IL-6 at 2 hours after percutaneous nephrolithotomy can early diagnose urosepsis, reduce the severity of postoperative urosepsis, shorten the postoperative hospital stay, and improve the prognosis.