The purpose of this study was to examine the contribution of the location-tracking information to the energy expenditure during the soccer-based intermittent sprint run. Twenty-four young healthy men participated in the present investigation (1st experiment: 14 men, 2nd experiment 10 men). In the 1st experiment, the sprint runs were 12 times of the 10 m (SS10), or 6 times of the 20 m (SS20), or td 3 times of the 40 m (SS40). In the 2nd experiment, the sprint runs 15 times of the 10 m (SS), or 15 times of the 10 m of the sprint run with change of direction for 90, 135, 180 degrees (CD90, CD135 and CD180). All course of the experiment, the expired gas was analyzed using the portable gas analyzer. The running index including speed, acceleration, distance, were calculated at 15 Hz interval based on the location-tracking information using the portable global positioning system. The intermittent run with the straight sprint run expended significantly larger calorie compared with that without the straight sprint (p < 0.001). The intermittent run with the changes of direction expended significantly larger calorie compared with that without the changes of direction (p < 0.05). A multiple stepwise regression analysis revealed that body weight, the acceleration and the change of direction were significant determinants of the energy expenditure and accounted for 75% of the total variance. These results indicate that the magnitude of the acceleration is significant predictor for the energy expenditure of the intermittent exercise.