ObjectiveTo explore changes of lipopolysaccharide (LPS), lipopolysaccharide-binding protein (LBP), bactericidal/permeability-increasing protein (BPI), soluble CD14 (sCD14) in blood of cirrhosis patients and their clinical significance.MethodsSerum samples of 45 cirrhosis patients were detected with chromogenic limulus amebocyte lysate assay for LPS and detected with ELISA for LBP, BPI and sCD14. While, serum samples of 15 normal subjects were used as controls.ResultsLevels of LPS, LBP, BPI and sCD14 in blood of cirrhosis patients with liver function being grade A, B and C were significantly higher than that in normal subjects. Also, those indexes fore mentioned were obviously higher in died cirrhosis patients than that in survived cirrhosis patients.Conclusion High levels of LBP, LPS and relative deficiency of BPI in cirrhosis patients accompanied with intestinal endotoxemia (IETM) may significantly increase the sensitivity of body to endotoxin.

ObjectiveTo explore the molecular mechanism of protective effects of recombinant human interleukin-11( rhIL-11) pretreatment on intestinal ischemia/reperfusion (I/R) injury of rats.MethodsThe I/R model of rat was produced by clampi ng the superior mesenteric artery for 1 hour, animals were divided randomly into the normal control group (A), I/R group (B), normal saline control group? and rhIL-11 pretreatment group (D). In group C and D, animals were administered w ith saline(0.25ml/rat/day)or rhIL-11 (600μg/kg/day) two days before the oper ation. Rats in different groups were sacrificed at 6 hours and 24 hours after re perfusion respectively. Intestinal apoptosis was detected by TUNEL staining, and the expression of bcl-2, caspase 3 and caspase 8 were determined by immunohist ochemistry. Meanwhile pathologic pictures were detected by hematoxylin-eosin (HE) staining.ResultsHE and TUNEL examinations showe d that in group D, the intestinal barrier was damaged obviously slighter than th at in the group B and group C, with decreased apoptosis cells, meanwhile, expres sion levels of caspase 3 and caspase 8 were lower, and bcl-2 was higher than th e group B and group C.Conclusions The protective e ffect of rhIL-11 pretreatment on rat intestinal I/R injury might be caused by t hat the expression of activities of caspase 3 and caspase 8 are inhibited and b cl-2 is activated.

Objective To study the relationship between the irritable bowel syndrome and bacterial overgrowth in the intestines.Methods Forty-nine cases of irritable bowel syndrome presenting as diarrhea or constipation were tested by the lactose-functose hydrogen breath test to determine the oral-cecal passage time and positive rate was noted.Of the cases with positive tests ten were selected for treatment with prepulsid for one week,and the pre-and post-treatment passage times were compared.Results In regard to passage time both diarrhea and constipation types of the irritable bowel syndrome showed prolongation compared with controls(P0 05),and improvement after treatment compared with pre-treatment,diarrhea was also improved by treatment.Conclusion Intestinal dysfunction can lead to bacterial overgrowth in the intestines,which can be a cause of the irritable bowel syndrome.

Objective To investigate the prevalence and characteristic alterations of the peptic ulcer in Beijing area. Methods Retrospective analysis of clinical data of peptic ulcer collected from 29 hospitals of Beijing area in 1999. Results 8 832 cases of peptic ulcer were found from 64 874 cases of gastroscopy with detecting rate of 13.61%. Among these peptic ulcer patients,6 179 cases were found to have duodenal ulcer,accounting for 69.96% of the total cases of peptic ulcer;2 058 cases gastric ulcer,23.30%of the total cases with the ratio of 3:1 between these two subgroups.The rest 595 cases of other ulcer types , including complex ulcer and marginal ulcer accounted for 6.74% of the total cases.The detecting rates of peptic ulcer were 13.16%,19.11%, 13.23%,13.55%,20.59%in the tertiary hospitals, secondary hospitals (P

Objective To select a practical and reliable method in cleaning and disinfecting endo-scope, and to investigate the efficacy of endoscope disinfection. Methods A study was done on the disinfection efficacy using or not using enzyme cleaning plus 2% glutaraldehyde immersion, and disinfection efficacy of automated washer/disinfectors for endoscopes at tertiary hospitals in Beijing. Results In 301 cases of en-doscopes cleaned with enzyme ( Epizyme rapid multi enzyme cleanse) and immersed in 2% glutaraldehyde for 7 minutes, culture was negative in 283 cases (94. 02% ) ; in 155 cases without enzyme cleaning but with immersion for 7 minutes in 2% glutaraldehyde, culture was negative in 137 cases (88. 39 % ). Taking 20 or less bacteria per endoscope as the eligibility criterion, there were 298(99.00% ) and 147(94. 84% ) cases eligible in enzyme treated and enzyme untreated group respectively , P

Objective To study the clinical characteristics of gastrointestinal (GI) hemorrhage in SARS and factors affecting its occurrence. Methods Retrospective study on clinical data of SARS cases complicated with gastrointestinal hemorrhage admitted to our hospital. Results Of 220 SARS cases gastrointestinal hemorrhage occurred in 20 patients (9% ) , with 13 males, 7 females, age ranged 19 -83 years (50?20) , onset at the 5th to 35th day of illness (22?9) , hematemesis/melena in 5 cases, positive occult blood test in 15. After treated with famotidine, omeprazole, reptilase and thrombin bleeding stopped in 16 cases within 7 to 10 days. Four cases died of respiratory failure. Rate of hemorrhage was lower in patients receiving prophylactic therapy with H2 receptor blocker famotidine than those without prophylactic therapy (P

Objective To explore the influence of different preservation conditions and time period on the testing results of Alzheimer's disease(AD) associated neuronal thread protein(AD7C-NTP) in urine specimen.Methods From Oct.2015 to Jan.2016,urine specimen were collected from 50 AD patients,and divided into three groups,according to the different storage temperature,including room temperature group,4 ℃ group and-20 ℃ group.Preservatives were added into specimen of 4 ℃ preservation group and-20 ℃ preservation group.AD7C-NTP level was detected at different preservation time of all specimen.Results The testing results of AD7C-NTP in specimen of room temperature group and 4 ℃ groups,detected within three days,were not significantly different with initial detection value(P>0.05).After seven days,the testing results in specimen of 4 ℃ group were not significantly different with initial detection value(P>0.05).However,after one day,the testing results in specimen of-20 ℃ group were significantly different with initial detection value(P<0.05).Contrast with 4 ℃ without preservative group,the adding of preservative could not increase the stability of AD7C-NTP.The adding of preservative in specimen of-20 ℃ could obviously increase stability,but the deviation of testing results was beyond acceptable limits.Conclusion 4 ℃ without preservatives could be the optimal storage condition for detection of AD7C-NTP in the urine.

OBJECTIVETo assess the prevalence of colorectal cancer (CRC) in Beijing and the reliability of the sequential fecal occult blood test (SFOBT) for CRC screening.

METHODSOf the natural population (48 100 persons) in several Beijing communities, we screened 26 827 persons with age over 30 using the SFOBT screening program, Guaiacum Fecal Occult Blood Test (GFOBT), Immuno Fecal Occult Blood Test (IFOBT), and colonoscopies.

RESULTSThe screening rate of the population was 74%. The positive rate of SFOBT was 5.6%. The prevalence of CRC in the entire population of Beijing was therefore calculated to be 36.57/10(5). Of 12 CRC detected patients, 4 cases were in stage Dukes A (33.33%), 7 cases in stage Dukes B (58.33%), only 1 case (8.34%) in stage Dukes C.

CONCLUSIONSThe prevalence of CRC in Beijing is one of the highest in China. Individuals at high risk for CRC or those over 50 years of age should be considered as primary candidates for screening. SFOBT screening is a cost-effective and reliable method for early detection of CRC.

Adult ; Aged ; Aged, 80 and over ; China ; Colorectal Neoplasms ; diagnosis ; Female ; Humans ; Male ; Middle Aged ; Occult Blood