1.Investigation of satisfactory rate in patients with body dysmorphic disorder after cosmetic surgery.
Guan-Hua YANG ; Yue-Jia LUO ; Tai-Chao DU ; Quan-You LEI ; Yong-Xue XIE
Chinese Journal of Plastic Surgery 2008;24(2):151-152
OBJECTIVETo explore the satisfactory rate in patients with body dysmorphic disorder (BDD) after cosmetic surgery.
METHODSWe designed a questionnaire to investigate the postoperative satisfactory rate in patients with BDD and without BDD.
RESULTSThere was significant difference in postoperative satisfactory rate between patients with BDD and without BDD.
CONCLUSIONThe patients with BDD have a lower satisfactory rate, which is even worse after repeated surgery.
Adult ; Body Dysmorphic Disorders ; psychology ; Body Image ; Female ; Humans ; Male ; Middle Aged ; Patient Satisfaction ; Surgery, Plastic ; Surveys and Questionnaires
2.Validation of a cell infection-based quantitative RT-PCR for evaluation of rotavirus vaccine potency
Yueyue LIU ; Yunqi ZHANG ; Yan LIU ; Yunjin WANG ; Mingqiang WANG ; Yan ZHAO ; Jialiang DU ; Chao MA ; Xu ZHOU ; Tai GUO
Chinese Journal of Microbiology and Immunology 2019;39(7):532-537
Objective To validate a cell infection-based quantitative RT-PCR for evaluating the potency of rotavirus vaccine. Methods According to the ICH ( the International Council for Harmonization) Harmonised Tripartite Guideline, the method was validated for its specificity, accuracy, precision, linearity and robustness. Results The method had good specificity as it could only amplify and detect the corre-sponding type of rotavirus strain. The recovery rates for determining the potency against rotaviruses of G2, G3 and G4 types were 97% to 108%. The percent coefficient of variation ( CV) of both intra-plate and in-ter-plate precision was≤2. 62%, while the intraday and interday CV was≤1. 76% and≤2. 27%, respec-tively. The CV between the two experimenters was≤7. 68%. The linearity range of the method was 4. 4-6. 5 UI for G2 type rotavirus, 3. 9-8. 3 UI for G3 type and 3. 5-8. 1 UI for G4 type. Good robustness was observed using the cells of 140 to 160 generations. Conclusions The cell infection-based quantitative RT-PCR was shown to have satisfactory specificity, accuracy, precision, linearity and robustness, suggesting that it was a suitable method for evaluating the potency of multivalent rotavirus live vaccines.
3.An area CCD-based detection system for up-converting phosphor immune lateral flow assay
Chao LI ; Zi-Jiang YANG ; Yao-Hua DU ; Tai-Hu WU ; Feng CHEN
Military Medical Sciences 2017;41(11):906-911
Objective To make portable the detector for up-converting phosphor (UCP) immune lateral flow assay portable and increase the test precision by developing an area CCD-based detection system for UCP immune lateral flow assay.Methods The excitation light source was a 980 nm and 300 mW laser diode.In order to decrease the error induced by the un-uniformity of laser irradiation,a uniformity mirror was inserted in the beam and a calibration algorithm was optimized.The residual error from the un-uniformity irradiation was less than 1%.Results Thanks to the adjustability of the exposure time,the dynamic range of detection of the system was as high as 106 dB.The repeat test error for the very low signal was 1% (variation coefficient).The linear correlation coefficient between the tested T/C value and sample concentration was 0.998.Conclusion Compared to is traditional instrument,this detection system is static,portable and highly precise.