No abstract available.
Cryptococcosis* ; Hybridomas* ; Immunity, Cellular* ; T-Lymphocytes*

No abstract available.
Cryptococcosis* ; Hybridomas* ; Immunity, Cellular* ; T-Lymphocytes*

Priapism is a condition of prolonged erection, unassociated with sexual stimulation, which is usually painful We experienced two cases of idiopathic priapism, the one was cured by the corporal irrigation with normal saline containing heparin through the glans tip and the other by the corpora cavernosa-saphenous vein shunt. Postoperatively potency in these two cases was obtained.
Heparin ; Priapism* ; Veins

BACKGROUND: Neurocardiogenic syncope (NCS) is the most common type of neurally mediated syncopes. Though presyncopal symptoms may represent hypoperfusion in posterior circulation, studies for evaluating the cerebrovascular response of posterior circulation in patients with NCS were scanty. We evaluated the cerebrovascular response of the posterior circulation using transcranial Doppler monitoring in patients with NCS. METHODS: We measured the blood flow velocity (BFV) of the middle cerebral artery (MCA) and contralateral posterior cerebral artery (PCA) simultaneously in 11 patients with a diagnosis of NCS both at rest and during the head-up tilt test (HUT) for 5 minutes. The mean arterial pressure (MAP), pulse rate (PR), and end-tidal pCO2 (EtCO2) were also monitored. The obtained data were compared with those of 17 normal control subjects. RESULTS: The syncope patients showed a significantly larger decline in BFV of PCA (P<0.05) and a larger increase in pulsatility indexes of MCA and PCA during HUT compared to those of control subjects. In control subjects BFV of PCA decreased less than those of MCA. On the contrary, in patients with NCS, those of PCA decreased more. These different regional cerebrovascular responses to HUT were accompanied with neither presyncopal symptoms nor decrease in MBP. CONCLUSIONS: The regional cerebrovascular response to HUT in NCS patients was different in the PCA flow. Impairment of autonomic neural control of posterior circulation may play an important role in the pathophysiology of NCS.
Arterial Pressure ; Blood Flow Velocity ; Heart Rate ; Humans ; Middle Cerebral Artery ; Passive Cutaneous Anaphylaxis ; Posterior Cerebral Artery ; Syncope ; Syncope, Vasovagal

No abstract available.
Kidney Transplantation*

Tourniquets are often used for surgery on the extremities to provide a bloodless surgical field. However, tourniquets may cause nerve and other tissue injuries.While the tourniquet is inflated, metabolic changes occur in the ischemic limb, chages that include increased PaCO2, lactic acid and pottasium, and decreased levels of PaO2 and pH. Deflation of the tourniquet results in the release of these products of ischemia into the general circulation. The resultant decreases in arterial pH and PaO2 and increased in arterial lactic acid, potassium, PaCO2 and P(ET)CO2 are associated with significant decrease in mean arterial and central venous pressure and increase in heart rate. Since Pa is one of the important factor to control the cerebral blood flow and intracranial pressure, a sudden increase in PaCO2 immediately after toumiquet deflation may cause detrimental effects in head injured patients with elevated intracranial pressure. The purpose of this study was investigate the effect of the degree of hyperventilation by means of increased minute ventilation by 25%, 50% and 75% respectively, after deflation of toumiquets to keep P(ET)CO2 at former levels, for twenty minutes period, with arterial blood gas analysis, endtidal PaCO2 and vital signs was monitored serially. We compared normal minute ventilation group I with increased minute ventilation groups(II, III and IV) before and after deflation of tourniquet. In summary, we calculated from our data that increasing minute ventilation by more than 50% for 5 minutes following tourniquet deflation could prevent P(ET)CO2 from increasing more than 1.5-6.5 mmHg in all patients.
Blood Gas Analysis ; Central Venous Pressure ; Extremities ; Head ; Heart Rate ; Humans ; Hydrogen-Ion Concentration ; Hyperventilation ; Intracranial Hypertension ; Intracranial Pressure ; Ischemia ; Lactic Acid ; Potassium ; Tourniquets* ; Ventilation* ; Vital Signs*

An insulin-producing cell line, HE, was derived from immortalized human pancreatic p-cells transformed by SV40 DNA, To make serum free medium specific for the cells, it was necessary to select basal medium, hormones, growth factors and coating materials. First of all, human insulin-producing cell line was adapted serially to the culture medium containing minimum required (1%) fetal calf serum (HB-1) from 10% fetal calf serum, The cells had a tendency to attach to the culture dishes coated with collagen or gelatin in the serum-free medium and showed a stronger attachment to collagen than to gelatin, For the basal medium selection, medium containing an equal mixture of RPMI 1640, Dulbecco's modified Eagle's medium, and Ham's F-12 (RDF) was shown to be the most suitable for the cells among 7 differently mixed basal media, The cells exhibited highly positive response to 7 factors including insulin-transferrin-selenium (ITS)complex in the basal medium. In addition, other 16 factors also influenced the cell growth positively, In contrast, 4 factors including aldosterone inhibited the cell growth, Cell growth was enhanced remarkably when the cells were cultured in the serum-free medium supplemented with all the seven positive factors, compared with in RDF serum-free medium. ITS and linoleic acid-BSA were found to be essential for the cell growth. The growth was much higher in the medium containing 6 strong positive factors without either dexamethasone, glucagon, or thyrocalcitonin than in the medium containing all the 7 strong positive factors, It is suggested that the interaction between the individual positive factors must be considered in choosing the factors with which the serum-free medium is supplemented.
Aldosterone ; Calcitonin ; Cell Line* ; Collagen ; Dexamethasone ; DNA ; Gelatin ; Glucagon ; Humans* ; Insulin ; Intercellular Signaling Peptides and Proteins*

No abstract available.
Pyelonephritis*

Scar endometriosis is one of the rare conditions among the extrapelvic endometriosis, but it often occurs after pelvic operation and episiotomy. Four cases of scar endometriosis following Cesarean section and episiotomy were exp- erienced and treated with complete surgical excision. Therefore we reported these cases with concerned literatures.
Cesarean Section ; Cicatrix* ; Endometriosis* ; Episiotomy ; Female ; Pregnancy

PURPOSE: Peripheral blood stem cell transplantation (PBSCT) has recently been used to rescue from myelosuppression following high-dose chemo-radiotherapy in patients with leukemia and solid tumor. Nevertheless, few data are still available on PBSC collection in pediatric patients, owing to technical problems. The time of stem cell harvest and the mobilization regimen may play important roles in terms of achieving adequate numbers of stem cells by leukapheresis. In this study, we analyse; 1) the technical aspects of leukapheresis as to feasibility and safety, 2) the optimal timing for PBSC collection after cytokine-based mobilizing regimens, 3) the engraftment kinetics. Method: A total of 93 leukapheresis was performed 22 children by Fenwall CS 3000 continuous cell separator, of whom 15 children weighed less than 25 kg. To mobilize hematopoietic stem cells into circulation, hematopoietic growth factor plus chemotherapy were used. Nineteen patients underwent autologous peripheral blood stem cell transplantation. RESULTS: The mean body weight was 25.3 kg (range: 10 to 56 kg). A total of 3 to 12 L of blood was processed (mean 265.4 65.9 mL/kg) for 2.5 to 5 hours (mean 3.15 hours). Extracorporeal line was primed with packed red blood cells below 25 kg. Serious morbidity was not noted. Each apheresis products contained a mean of 2.41 1.63x108 mononuclear cells/kg, 2.83 3.40x106 CD34 cells/kg, 9.30 10.3x104 colony forming unit (CFU-GM)/kg, respectively. Absolute neutrophil count (r=0.38, P<0.01) and CD34 cell count (r=0.65, P<0.001) on the day of leukapheresis seemed to predict the CFU-GM count collected in leukapheresis. A significant statistical correlation between the number of infused CFU-GM and the time to achieve an absolute neutrophil count of greater than 500/mm3 (P<0.01) was found. CONCLUSION: Leukapheresis for PBSCT seemed to be feasible and reliable in pediatric patients, conferring no major additional risks than adult patients, only if red cells are primed in extracorporeal line for small children. Absolute neutrophil count and CD34 cell number seemed to predict the timing of leukapheresis. In the PBSCT patient, engraftment was influenced by the infused CFU-GM count and bone marrow environment.
Adult ; Blood Component Removal ; Body Weight ; Bone Marrow ; Cell Count ; Child ; Drug Therapy ; Erythrocytes ; Granulocyte-Macrophage Progenitor Cells ; Hematopoietic Stem Cells ; Humans ; Kinetics* ; Leukapheresis ; Leukemia ; Neutrophils ; Peripheral Blood Stem Cell Transplantation ; Stem Cells*