1.Inhibition of porcine pancreatic phospholipase A2 by JE-1 andlipocortin: effective binding to phosphatidylcholine.
Kyung Soo NAM ; In Seong CHOE ; Tai Wha CHUNG
Korean Journal of Immunology 1991;13(2):225-230
No abstract available.
Phosphatidylcholines*
;
Phospholipases A2*
;
Phospholipases*
2.A Case of Bilateral Simultaneous Hypertensive Intracerebral Hemorrhage in Basal Ganglia.
Se Hoon KIM ; Tai Hyoung CHO ; Hung Seob CHUNG ; Hoon Kap LEE ; Jeong Wha CHU ; Ki Chan LEE
Journal of Korean Neurosurgical Society 1995;24(6):682-688
Spontaneous intracerebral hemorrhage is one of the most devastating forms of cerebrovascular disease in the field of neurosurgery, and is most frequently associated with the hypertension in the distribution of the penetrating vessels, or can be secondary to other factors, like aneurysm, arteriovenous malformation, glial tumor, metastasis, infarction, anticoagulation therapy, coagulation disorders such as leukemia or thrombocytopenia. Although many cases of recurrent hypertensive intracerebral hemorrhage have been reported, bilateral simultaneous hemorrhage has not been reported. The authors report a case of bilateral simultaneous hypertensive intracerebral hemorrhage in a 62-year-old male, which was treated by conservative management.
Arteriovenous Fistula
;
Basal Ganglia*
;
Cerebral Hemorrhage
;
Hemorrhage
;
Humans
;
Hypertension
;
Infarction
;
Intracranial Hemorrhage, Hypertensive*
;
Leukemia
;
Male
;
Middle Aged
;
Neoplasm Metastasis
;
Neurosurgery
;
Thrombocytopenia
3.Production and characterization of HPV16 recombinant capsid protein L2 in E.coli.
Joo Hyun KANG ; Young Hee LEE ; Mi Ri YOON ; Hee Shick YN ; Dur Han KWON ; Yong Kyung CHOE ; Soon Hee PARK ; In Seong CHOE ; Roh Pal CHUNG ; Tai Wha CHUNG ; Kyung Soo HAHM
Journal of the Korean Society of Virology 1993;23(2):223-231
No abstract available.
Capsid Proteins*
;
Capsid*
4.Spontaneous Rupture of Mediastinal Teratoma into Adjacent Tissues.
Jeong Bae JEON ; Chung Hwan CHUNG ; Tai Hoon MOON ; Jae Wha CHO ; Jeong Seon RYU ; Seung Min KWAK ; Hong Lyeol LEE ; Chul Ho CHO ; Hye Seung HAN
Tuberculosis and Respiratory Diseases 1999;47(3):400-405
Mediastinal teratomas are rare and represent less than 10 per cent of all mediastinal tumors. Almost all arise in the anterosuperior mediastinal compartment, and most symptoms, when present, result from compression of adjacent structures. They contain different tissues derived from all three germinal layers, with the prevalence of ectodermal elements which can include hair, teeth and sebaceous material. Benign teratomas may rupture into adjacent organs. Up to 36% of all mediastinal teratomas rupture, most frequently into the lung and bronchial tree, followed by the pleural space, pericardial space, or great vessels. The signs and symptoms of a ruptured teratoma vary with the structures involved. We report a case of mediastinal teratoma ruptured spontaneously in a 18 year old female who experienced 4 or 5 times of hemoptysis for 1 year and sudden onset of pleural effusion, pericardial effusion and pneumonia.
Adolescent
;
Ectoderm
;
Female
;
Hair
;
Hemoptysis
;
Humans
;
Lung
;
Mediastinum
;
Pericardial Effusion
;
Pleural Effusion
;
Pneumonia
;
Prevalence
;
Rupture
;
Rupture, Spontaneous*
;
Teratoma*
;
Tooth
5.Expression of human papillomavirs 18 capsid protein L2 in insect cells.
Byung Tae KANG ; Seung Won JIN ; Eun Kyung YANG ; Dur Han KWON ; Soon Hee PARK ; C Young KANG ; Wang Don YOO ; Hyun Su KIM ; In Seong CHOE ; Tai Wha CHUNG
Journal of the Korean Society of Virology 1993;23(2):233-238
No abstract available.
Capsid Proteins*
;
Capsid*
;
Humans*
;
Insects*
6.Annual Report on External Quality Assesment in Hematology in Korea (2003).
Jong Woo PARK ; Gye Cheol KWON ; Youn Bo PARK ; Chan Jeoung PARK ; Hyo Soon PARK ; Kyung Soon SONG ; Dong Wook YANG ; Do Hoon LEE ; Woong Soo LEE ; Wha Soon CHUNG ; Han Ik CHO ; Hyun Chan CHO ; Hyun Sook CHI ; Jong Tai CHOI ; Kyung Ja HAN
Journal of Laboratory Medicine and Quality Assurance 2004;26(1):29-45
Four trials of external quality assessment in diagnostic hematology were performed in 2003 with about 430 participating laboratories in Korea. We performed quality assessment for white blood cell count, hemoglobin, red blood cell count, platelet count, white cell differential count, red blood cell morphology and coagulation test. The response rate was more than 95%. The performance of quality assessment appeared to be gradually improved year by year.
Equidae*
;
Erythrocyte Count
;
Erythrocytes
;
Hematology*
;
Korea*
;
Leukocyte Count
;
Platelet Count
7.Annual Report on External Quality Assesment in Hematology in Korea (2004).
Jong Woo PARK ; Gye Cheol KWON ; Youn Bo PARK ; Chan Jeoung PARK ; Hyo Soon PARK ; Kyung Soon SONG ; Dong Wook YANG ; Do Hoon LEE ; Woong Soo LEE ; Wha Soon CHUNG ; Han Ik CHO ; Hyun Chan CHO ; Hyun Sook CHI ; Jong Tai CHOI ; Tae Eun JEONG ; Kyung Ja HAN
Journal of Laboratory Medicine and Quality Assurance 2005;27(1):21-36
Four trials of external quality assessment in diagnostic hematology were performed in 2004 with about 440 participating laboratories in Korea. We performed quality assessment for white blood cell count, hemoglobin, red blood cell count, platelet count, white cell differential count, red blood cell morphology and coagulation test. The response rate was more than 96%. The coefficients of variation in hemoglobin and RBC number was stable but variable in platelet number and WBC number according to measuring cell counts. Blood coagulation study was performed twice. Test results show wide variation according to measuring machine and reagents.
Blood Coagulation
;
Cell Count
;
Equidae*
;
Erythrocyte Count
;
Erythrocytes
;
Hematology*
;
Indicators and Reagents
;
Korea*
;
Leukocyte Count
;
Platelet Count
8.Expression patterns of green fluorescent protein (GFP) after the intravenous injection with adenovirus vector in mouse organs.
Jong Youl JIN ; Chi Won SONG ; Jeana KIM ; Hee Jin LEE ; Tai Gyu KIM ; Chi Wha HAN ; Hyun Seok EOM ; Soo Jeong PARK ; Dae Chul JEONG ; Nak Gyun CHUNG ; Soh Yeon KIM ; Young Hoon SEO
Korean Journal of Medicine 2001;61(5):537-545
BACKGROUND: The green fluorescent protein (GFP) from jelly fish, Aequorea victoria, has become a versatile reporter for monitoring gene expression in a variety of cells and organisms. Using GFP as a marker protein we studied whether there are any differencies in the expression patterns among organs in mouse after intravenous injection of adenovirus vectors with GFP gene. METHODS: Recombinant E1, E3-defective type 5 adenovirus vectors (2x10(8)/mouse) with CMV promoter and GFP gene were injected into mice via tail vein. On 3, 6, 9, 14, 21, 28 days after gene transfer, 5 mice per experiments were sacrificed by cervical dislocation and obtained liver, lung, heart, kidney, spleen, small intestine and bone. Half of them were examined by optical microscope after H-E stain. Another half were examined by fluorescent microscope after frozen section. Western blottings were done for each samples with anti-GFP monoclonal antibody and obtained GFP bands were quantitatively compared using Gel-Doc (Bio-Rad, USA) image analyzer. RESULTS: In all organs that we obtained, expression of GFPs are noticed 3 days after gene transfer and reached a maximum around 9th to 14th days, after then the intensities are slightly decreased but maintained until 28th days as determined by Western blotting. On fluorescent microscopic examination, GFPs are well and most frequently expressed on lung among all the examined organs. There are little expression of GFPs on liver parenchymal area around the sinusoids and central veins, although patchy expression of GFPs are observed along the liver capsules. GFPs are highly expressed around the splenic trabecula area but splenic pulp area, it is very sparsely expressed. GFPs are more frequently and highly expressed around the renal tubular area than gromerular area in kidneys. In small intestine, GFPs are expressed on mid portion of microvilli. GFPs are not expressed on myocardium except scanty expression on endocardium. Bone marrow showed GFPs but precise localization is difficult because bony spicules mashed bone marrow during the preparation of frozen section. No specific pathologic lesions possibly related with adenovirus administration are observed on microscopic examination of H-E stained specimens. CONCLUSIONS: GFPs can be detected in cells without the fixing and staining and a good marker to studying the kinetics and persistence of adenovirus mediated gene therapy. And there are different GFP expression patterns according to the organs after intravenous injection of adenovirus vectors with GFP gene in mouse.
Adenoviridae*
;
Animals
;
Blotting, Western
;
Bone Marrow
;
Capsules
;
Dislocations
;
Endocardium
;
Fluorescence
;
Frozen Sections
;
Gene Expression
;
Genetic Markers
;
Genetic Therapy
;
Heart
;
Injections, Intravenous*
;
Intestine, Small
;
Kidney
;
Kinetics
;
Liver
;
Lung
;
Mice*
;
Microvilli
;
Myocardium
;
Spleen
;
Veins
;
Victoria
9.Inhibition of Graft Versus Host Disease Using CD4+ CD25+ T Cells Induced with Interleukin-2 in Mismatched Allogeneic Murine Hematopoietic Stem Cell Transplantation.
Jae Ho HYUN ; Dae Chul JEONG ; Nak Gyun CHUNG ; Soo Jeong PARK ; Woo Sung MIN ; Tai Gyu KIM ; Byung Ock CHOI ; Won Il KIM ; Chi Wha HAN ; Hack Ki KIM
Immune Network 2003;3(4):287-294
BACKGROUND: In kidney transplantation, donor specific transfusion may induce tolerance as a result of some immune regulatory cells against the graft. In organ transplantation, the immune state arises from a relationship between the immunocompromised graft and the immunocompetent host. However, a reverse immunological situation exists between the graft and the host in hematopoietic stem cell transplantation (HSCT). In addition, early IL-2 injections after an allogeneic murine HSCT have been shown to prevent lethal graft versus host disease (GVHD) due to CD4+ cells. We investigated the induction of the regulatory CD4+CD25+ cells after a transfusion of irradiated recipient cells with IL-2 into a donor. METHODS: The splenocytes (SP) were obtained from 6 week-old BALB/c mice (H-2(d)) and irradiated as a single cell suspension. The donor mice (C3H/ He, H-2(k)) received 5x10(6) irradiated SP, and 5,000 IU IL-2 injected intraperitoneally on the day prior to HSCT. The CD4+CD25+ cell populations in SP treated C3H/He were analyzed. In order to determine the in vivo effect of CD4+CD25+ cells, the lethally irradiated BALB/c were transplanted with 1x10(7) donor BM and5x10(6) CD4+CD25+ cells. The other recipient mice received either 1x10(7) donor BM with 5x10(6) CD4+ CD25- cells or the untreated SP. The survival and GVHD was assessed daily by a clinical scoring system. RESULTS: In the MLR assay, BALB/c SP was used as a stimulator with C3H/He SP, as a responder, with or without treatment. The inhibition of proliferation was 30.0 13% compared to the control. In addition, the MLR with either the CD4+CD25+ or CD4+CD25- cells, which were isolated by MidiMacs, from the C3H/He SP treated with the recipient SP and IL-2 was evaluated. The donor SP treated with the recipient cells and IL-2 contained more CD4+CD25+ cells (5.4+/-1.5%) than the untreated mice SP (1.4+/-0.3%)(P<0.01). There was a profound inhibition in the CD4+CD25+ cells (61.1+/-6.1%), but a marked proliferation in the CD4+CD25- cells (129.8+/-65.2%). Mice in the CD4+CD25+ group showed low GVHD scores and a slow progression from the post-HSCT day 4 to day 9, but those in the control and CD4+CD25- groups had a high score and rapid progression (P<0.001). The probability of survival was 83.3% in the CD4+CD25+ group until post-HSC day 35 and all mice in the control and CD4+CD25- groups died on post-HSCT day 8 or 9 (P=0.0105). CONCLUSION: Donor graft engineering with irradiated recipient SP and IL-2 (recipient specific transfusion) can induce abundant regulatory CD4+CD25+ cells to prevent GVHD.
Animals
;
Graft vs Host Disease*
;
Hematopoietic Stem Cell Transplantation*
;
Hematopoietic Stem Cells*
;
Humans
;
Interleukin-2*
;
Kidney Transplantation
;
Mice
;
Organ Transplantation
;
T-Lymphocytes*
;
Tissue Donors
;
Transplants*
10.Annual Report on External Quality Assesment in Hematology in Korea (2002).
Jong Woo PARK ; Gye Cheol KWON ; Hyo Soon PARK ; Woong Soo LEE ; Chan Jeoung PARK ; Kyung Soon SONG ; Dong Wook YANG ; Yon Bo PARK ; Do Hoon LEE ; Wha Soon CHUNG ; Han Ik CHO ; Hyun Sook CHI ; Jong Tai CHOI ; Kyung Ja HAN
Journal of Laboratory Medicine and Quality Assurance 2003;25(1):35-49
Four trials of external quality assessment in diagnostic hematology were performed in 2002 with about 400 participating laboratories in Korea. We performed quality assessment for white blood cell count, hemoglobin, red blood cell count, platelet count, reticulocyte count, white cell differential count, and red blood cell morphology test. The response rate was more than 90%. The performance of quality assessment appeared to be gradually improved year by year.
Equidae*
;
Erythrocyte Count
;
Erythrocytes
;
Hematology*
;
Korea*
;
Leukocyte Count
;
Platelet Count
;
Reticulocyte Count