A 13 years old girl came to our department with complaints of multiple ulcerating and non-ulcerating nodules on her back and buttocks since 1 month prior to visit. Skin biopsy specimen showed diffuse monomorphous infiltration of leukemic cells in the dermis and subcutis. Bone marrow biopsy specimen showed changes of erythroleukemia. Treatment was initiated with combined chemotherapy which was a combination of cytosine arabinoside, adriamyein and 6 thioguanine. When combined chemotherapy was finished, she was in complete remission state and the nodules cured after 1 month of combined chemotherapy.
Adolescent ; Biopsy ; Bone Marrow ; Buttocks ; Cytarabine ; Dermis ; Drug Therapy ; Female ; Humans ; Leukemia* ; Leukemia, Erythroblastic, Acute* ; Skin ; Thioguanine ; Ulcer

In order to investigate the neurotoxic effect of methylmercury (MM) on cultured mouse spinal motoneuron cell line, NSC-19, neurotoxic effect of MM was evaluated by MTT assay after neurons were incubated with various concentrations of methylmercuric chloride (MMC) for 24 hours. In addition, neuroprotective effect of vitamin E against MMC-induced neurotoxicity was examined by MTT assay and neurofilament enzymeimmunoassay (EIA) in these cultures. The results were as follows : 1. MTT50 value was a concentration of 20µM methylmercuric chloride. 2. Methylmercuric chloride was toxic on cultured spinal motor neurons, NSC-19 cells in a time-and dose-dependent manner by severe decrease of cell viability. 3. Methylmercuric chloride induced the decrease of cell number and the loss of neuritis on these cultures. 4. Vitamin E remarkably increased the viabilily of cultured neurons damaged by methylmercury-induced neurotoxicity at a concentration of 250µM vitamin E. From above the results, it is suggested that methylmercury induces severe toxic effect on cultured mouse spinal motor neurons, NSC-19 cells, and the selective antioxidants such as vitamin E are effective in the neurotoxicity induced by methylmercury in these cultures.
Animals ; Antioxidants ; Cell Count ; Cell Culture Techniques ; Cell Line ; Cell Survival ; Intermediate Filaments ; Mice ; Motor Neurons* ; Neuritis ; Neurons ; Neuroprotective Agents ; Vitamin E* ; Vitamins*

To evaluate the effect of midkine (MK), neurotrophic factor on cultured mouse spinal motor neuron, NSC-19 which was inhibited by glucose oxidase (GO)-induced oxygen radicals, MTT assay and neurofilament enzymeimmunoassay were carried out after NSC-19 cells were preincubated with various concentrations of midkine for 2 hours prior to exposure of glucose oxidase. The results were as follows : 1. MK increased the rate of cell viability and neurofilamental development in a dose-dependent manner on motoneurons inhibited by glucose oxidase-induced oxygen radicals. 2. MTT50 value was 25 mU/ml GO. 3. GO-induced oxygen radicals were toxic on cultured motor neurons in a time and dose-dependent manner. 4. GO-induced oxygen radicals induced the decrease of cells in number and the loss of neurites in cultured mouse spinal motor neurons. From above the results, it is concluded that oxygen radicals are toxic in cultured mouse spinal motor neurons, and selective neurotrophic factors such as MK enhance the viability of motor neurons inhibited by oxygen radicals.
Animals ; Cell Survival ; Glucose ; Glucose Oxidase ; Intermediate Filaments ; Mice ; Motor Neurons* ; Nerve Growth Factor ; Nerve Growth Factors ; Neurites ; Oxidative Stress* ; Reactive Oxygen Species

No abstract available.
Humans ; Reproducibility of Results*

No abstract available.
Cryptococcus neoformans* ; Cryptococcus* ; Interleukin-2* ; Lymphocytes*

To elucidate the neurotoxic mechanism of methylmercury on cultured bovine oligodendrocytes, neurotoxic effect was estimated by MTT assay after cultures were exposed to various concentrations of methylmercuric chloride (MMC). In addition, neuroprotective effect of antioxidant, allopurinol agonist MMC-induced neurotoxicity was examined on these cultures. Exposure of cultured bovine oligodendrocytes to MMC showed less than 50% of the cell viability 24 hours after treatment with 35µM of MMC. And also, allopurinol blocked the neurotoxicity induced by MMC on these cultures. These results suggest that oxygen radicals involve in MMC-mediated neurotoxicity, and also seletive antioxidants such as allopurinol are effective in blocking the neurotoxicity induced by MMC on cultured bovine oligodendrocytes.
Allopurinol* ; Antioxidants ; Cell Survival ; Neuroprotective Agents ; Oligodendroglia ; Reactive Oxygen Species

No abstract available.
Urinary Tract Infections* ; Urinary Tract*

No abstract available.
Animals ; Capsaicin* ; Macrophages, Peritoneal* ; Mice*

This study was performed to investigate the factors related to tooth wear. For this study, 78 patients with temporomandibular disorders and 76 dental students without any signs and symptoms of temporomandibular disorders were selected as the patients group and as the normal group, respectively. Preferred chewing side, Angle's classification, lateral guidance pattern, head and shoulder posture wee observed clinically. Electromyographic activity of anterior temporalis and masseter muscle were recorded with BioEMG and occlusal status were recorded with T-Scan . Wear facet area of each tooth was measured from working model of upper arch corresponding to the occlusal status from T-Scan, Wear facet area were measured with planimeter in mm2. Total area were divided into incisal, canine, posterior tooth area. Anterior wearfacet area was incisor area plus canine area, and unilateral area was anterior area plus posterior area. The data collected were analyzed by SAS statistical program and the results of this study were as follows: 1. There was no significant difference between the two groups in total werafacet area, and male subjects showed tendency to have larger area in the normal group but female subjects showed tendency vice versa. 2. There was no significant difference related to preferred chewing side and Angle's classification, however, some difference was observed by lateral guidance pattern. Anterior wear facet area in subjects of canine guidance was the largest in the three subgroups. 3. Subjects with head tilting to right side had larger posterior and total area, and subjects with higher shoulder in right side had larger canine and anterior area than any other subgrous. 4. Electromyographic activity of masseter muscle was more correlated with wear facet area than anterior temporalis muscle, and tooth contact number and force were significantly correlated with wear facet area, but the most important factor affecting tooth attrition was age.
Dental Occlusion ; Female ; Head ; Humans ; Incisor ; Male ; Malocclusion ; Masseter Muscle ; Mastication ; Posture ; Shoulder ; Students, Dental ; Temporomandibular Joint Disorders ; Tooth Attrition ; Tooth Wear* ; Tooth*

No abstract available.
Animals ; Meperidine* ; Mice* ; Morphine* ; Naloxone*