At the blood transfusion and heamatology centre of Ho Chi Minh city, we performed phenotyping for 158 cases of acute leukemia diagnosed from 04/98 to 10/1998. Basing on these results, we go further to monitor residual disease depending on the presence of aberant antigens and DNA content. The enumeration of progenitor cell CD34+ also performed for leukemia patient during the bone marrow transplantation
Hematologic Diseases ; Biological Markers

The study was carried out on 188 cases of acute leukemia at Blood Transfusion and Hematology Center HCMC. All the patients were tested CD3, CD5, CD7, CD10, CD19, CD20, CD22, CD13, CD33, HLA- DR. The results showed that 87 B-ALL+ T-ALL, 94AML, 07 B-CLL with 15% of total cases disconcordanced between morphologic diagnosis and phenotyping diagnosis
Leukemia ; blood

A total of 54 cord blood collections were analysed for lymphocyte subsets. No significant differences in percentages of major lymphocyte subsets were found on CB in comparison with peripheral blood (CD 2+ 65%+/- 60, CD 3+ 69% +/- 5,4, CD 4+ 57%+/-7.6, CD 7+ 78% +/- 7.4 CD 8+ 16% +/- 6.1CD 16/56+ and CD 3+ 0.9% +/- 0.5, CD 19+ 16% +/- 3.9). The CD4: CD8 ratio is 3.56 compared with 1.4 in adult PB.CD 3+ with cytotoxic activity as determined by the expression of CD 56 are practically absent on CB.
Fetal Blood ; Lymphocyte Subsets

A total of 47 cord blood collection were analysed for CD 34+ progenitor cells. Results: volume (ml) without anticoagulant 64ml (40-90). Total NC 9.82x108+/- 0.45. Total CD 34+ 1.79x10 +/-1.4. Mean percentage of CD34+: 0.32%+/ 0.24. Given that a transplanted cell dose of 20x106 NC/kg body weight would be sufficient for hematopoiesis, 95% of all stored CB samples would be sufficient for patients up to 10 kg body weight and 36% for patients up to 40 kg body weight.
Fetal Blood ; Cells

In our search for natural soluble epoxide hydrolase (sEH) inhibitors from plants, an extract of the dried whole plants of Euphorbia supina Rafin was found to significantly inhibit sEH activity in vitro. Phytochemical investigation of E. supina resulted in isolation of 17 compounds (1 - 17), including triterpenes (1 - 4), phenolic compounds (5 - 8), and flavonoid derivatives (9 - 17). The structures of the isolated compounds were established mainly by extensive analysis of the 1D and 2D NMR, and MS data. All of the isolated compounds were evaluated for their sEH inhibitory activity. Among the isolated phenolic compounds, 8 was identified as a significant inhibitor of sEH, with an IC50 value of 15.4 +/- 1.3 microM. Additionally, a kinetic analysis of isolated compounds (2, 5, 8 - 11, 13, and 17) indicated that the inhibitory effects of flavonoid derivatives 10 and 11 were of mixed-type, with inhibitory constants (Ki) ranging from 3.6 +/- 0.8 to 21.8 +/- 1.0 microM, whereas compounds 2, 5, 8, 9, 13, and 17 were non-competitive inhibitors with inhibition Ki values ranging from 3.3 +/- 0.2 to 39.5 +/- 0.0 microM.
Euphorbia* ; Euphorbiaceae ; Inhibitory Concentration 50 ; Phenol ; Triterpenes

Phytochemical investigation of Kandelia candel resulted in the isolation of six triterpenes (1 - 5) and two glyceryl glycosides (6 and 7) and their structures were determined by comparing the spectroscopic data with those of reported values. In present study, we described the inhibitory effects of fractions and isolated compounds from K. candel on pro-inflammatory cytokines (IL-12 p40, IL-6, and TNF-alpha) production in lipopolysaccharide (LPS) stimulated bone marrow-derived dendritic cells (BMDCs). Results indicated that compounds 3, 6, and 7 showed potent inhibition on IL-6 production (IC50 values at less than 0.5 microM, respectively). Meanwhile, compounds 6 and 7 exhibited strong inhibitory effects on the production of TNF-alpha (IC50 values of 1.7 +/- 0.1 and 5.5 +/- 0.2 microM). Compounds 1 and 3 were also showed the inhibitory effects on IL-12 p40 production (IC50 values of 8.9 +/- 0.4 and 3.3 +/- 0.1 microM, respectively).
Cytokines ; Dendritic Cells ; Glycosides* ; Interleukin-12 ; Interleukin-6 ; Rhizophoraceae* ; Triterpenes* ; Tumor Necrosis Factor-alpha