1.Three Cases of Familial Benign Chronic Pemphigus.
Korean Journal of Dermatology 1979;17(5):379-383
Familial Renign Chronic Femphigus(FRCP) is rare, persistent and vesicobullous genodermatoai. Three casee of FRCP with typical clinical and histapathological findings are reported. The first case of FBCP was 27-year-oId man who had recurrent episodes of grou vesicles and rust with miId pruritue at the posterior neck, in both axillae and in inguinal area for 10 years. The second case was 49-year-old woman with skin lesions of recurrent persistent. vesicles, pustules on the erythematous skin, crust and lichenification on the flexural area of extrimeties, axillae, ingumal and neck area for 10 yeara. The third case was 49-year-old male patient who had recurrent pruritic grouped vesicles with crust on the neck and axillary area. In all three patients, skin eruptions usually began and or aggravated in the spring or summer and tend to heal spontaneously in the autumn and winter and showed positive Nikolsky sign. The only one case of the reported three casea had familiaI history of skin lesions similar to FRCP. Histopat,holag;ic finding of biopsy specimens from the vesicular eruption showed auprabasal vesiculation with acantholysis, lacunae and villi formation in all 3 cases.
Acantholysis
;
Axilla
;
Biopsy
;
Female
;
Humans
;
Male
;
Middle Aged
;
Neck
;
Pemphigus, Benign Familial*
;
Skin
2.A Study on the Detection of Herpes Simplex Virus using Nested PCR.
Mi Yeon CHOI ; Jin Woo YOO ; Tae Yeal CHOI ; Young Tae KIM
Korean Journal of Clinical Pathology 1997;17(5):764-771
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Base Sequence
;
Cercopithecus aethiops
;
DNA
;
Encephalitis
;
Glycoproteins
;
Herpes Simplex*
;
Herpesvirus 1, Human
;
Herpesvirus 2, Human
;
Keratitis
;
Kidney
;
Mouth Mucosa
;
Mucous Membrane
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Sexually Transmitted Diseases
;
Simplexvirus*
;
Skin
;
Uterine Cervical Neoplasms
3.A Study on the Detection of Herpes Simplex Virus using Nested PCR.
Mi Yeon CHOI ; Jin Woo YOO ; Tae Yeal CHOI ; Young Tae KIM
Korean Journal of Clinical Pathology 1997;17(5):764-771
BACKGROUND: Herpes simplex virus (HSV) is associated with a insignificant skin lesion, keratitis, encephalitis, congenital infection, sexually transmitted disease, or cervix cancer. There are two types of serogroup, HSV-1 and HSV-2. HSV-1 makes the lesion mainly on the above-waist area and HSV-2 makes the lesion mainly on the below-waist area. To diagnose the HSV infection, immunological or cultural methods usually have been used until now. But they are not satisfactory in terms of sensitivity, specificity, and ease of application. Recently the polymerase chain reaction (PCR) was developed. Because of the exponential nature of the amplification, this method can detect extremely small amount of DNA. We compared nested PCR with cultural method for HSV detection. METHODS: We obtained 61 specimens from the lesions of oral mucosa, face, and genital area. Samples were inoculated into the monolayer from the African green monkey kidney cell(Vero). When the slide showed cytopathic effect(CPE), HSV infection was confirmed, After extracting DNA from 61 samples, we amplified HSV DNA using nested PCR with the primers against the gene encoding glycoprotein (gD) of HSV-1 and HSV-2. RESULTS: We found 632 bp band after the 1st PCR round and 271 bp band after the 2nd PCR round with HSV-1 specific primers. HSV-2 revealed 428 bp band after the 1st PCR round and 231 bp band after the 2nd PCR round. Nested PCR showed analytical sensitivity at 10(-9) g of DNA in HSV-1 and 10(-10) g of DNA in HSV-2. Viral culture was positive in 36%, nested PCR detected HSV DNA sequence in 54% of samples. Nested PCR typed HSV, HSV-1 in 67%, HSV-2 in 39%, and mixed type in 6% of PCR-positive samples. All isolates from above-waist area were HSV-1. Seventy seven percent of 13 isolates from below-waist area were HSV-2 and 38% were HSV-1. CONCLUSIONS: Nested PCR offers a rapid, simple, and sensitive test for HSV infections of skin and mucosa.
Base Sequence
;
Cercopithecus aethiops
;
DNA
;
Encephalitis
;
Glycoproteins
;
Herpes Simplex*
;
Herpesvirus 1, Human
;
Herpesvirus 2, Human
;
Keratitis
;
Kidney
;
Mouth Mucosa
;
Mucous Membrane
;
Polymerase Chain Reaction*
;
Sensitivity and Specificity
;
Sexually Transmitted Diseases
;
Simplexvirus*
;
Skin
;
Uterine Cervical Neoplasms
4.The current status of blood component transfusion at the armed forces general hospital.
Jong Weon CHOI ; Young Chul OH ; Jin Tae SUH
Korean Journal of Blood Transfusion 1993;4(1):7-13
No abstract available.
Arm*
;
Blood Component Transfusion*
;
Hospitals, General*
5.A Case of Malignagr Lymphoma.
Young Ja CHOI ; Ho Suk SUNG ; Tae An CHUNG
Korean Journal of Dermatology 1979;17(2):143-147
A 28-year-old male patient is described who developed multiple erythematous nodules and papules on the face, upper chest, back, upper and lower extrimities, leading to extensive ia.filtration of the skin and lymph nodes. Histology from the involved lymph node and skin nodule revealed widely scattered patchy infiltration of abnormal. hyperchromatic polymorphous histiocytes in the dermis. The patient died at about 8 months after onset of the disease, in spite of antineoplastic treatment.
Adult
;
Dermis
;
Histiocytes
;
Humans
;
Lymph Nodes
;
Lymphoma*
;
Male
;
Skin
;
Thorax
6.14 Cases of Foreign Bodies in the Bladder.
Tae Young JUNG ; Nak Gyeu CHOI
Korean Journal of Urology 1997;38(1):76-80
Foreign bodies in genitourinary tract are not rare and most of them are present in the bladder. Various kinds of object are introduced into bladder through urethra by both male and female patients. Traditionally, they were introduced mainly for the purpose of sexual behavior. But recently like in our cases, many of them are inserted iatrogenically with development of endourologic procedure. We report 14 cases of bladder foreign bodies. Some of them are surprising and beyond common sense in kind of object and aim of insertion. Six cases were inserted by iatrogenic, 5 cases for masturbation, 2 cases by independently of patient`s intention by other person(s). One patient who had history of mental retardation did not remember when, how, why and by whom the foreign body had been introduced. All the foreign bodies could be diagnosed by history, symptom, X-ray and cystoscopy and removed by cystoscopic manipulation or cystostomy with ease and without complication.
Cystoscopy
;
Cystostomy
;
Female
;
Foreign Bodies*
;
Humans
;
Intellectual Disability
;
Intention
;
Male
;
Masturbation
;
Sexual Behavior
;
Urethra
;
Urinary Bladder*
7.14 Cases of Foreign Bodies in the Bladder.
Tae Young JUNG ; Nak Gyeu CHOI
Korean Journal of Urology 1997;38(1):76-80
Foreign bodies in genitourinary tract are not rare and most of them are present in the bladder. Various kinds of object are introduced into bladder through urethra by both male and female patients. Traditionally, they were introduced mainly for the purpose of sexual behavior. But recently like in our cases, many of them are inserted iatrogenically with development of endourologic procedure. We report 14 cases of bladder foreign bodies. Some of them are surprising and beyond common sense in kind of object and aim of insertion. Six cases were inserted by iatrogenic, 5 cases for masturbation, 2 cases by independently of patient`s intention by other person(s). One patient who had history of mental retardation did not remember when, how, why and by whom the foreign body had been introduced. All the foreign bodies could be diagnosed by history, symptom, X-ray and cystoscopy and removed by cystoscopic manipulation or cystostomy with ease and without complication.
Cystoscopy
;
Cystostomy
;
Female
;
Foreign Bodies*
;
Humans
;
Intellectual Disability
;
Intention
;
Male
;
Masturbation
;
Sexual Behavior
;
Urethra
;
Urinary Bladder*
8.Two Cases of Acute Febrile Neutrophilic Dermatosis (Sweet's Syndrome).
Young Ja CHOI ; Kyung Sool KWON ; Tae An CHUNG
Korean Journal of Dermatology 1980;18(4):327-333
We present two cases of acute febrile neutrophilic dermatosis developed in women aged 56 and 52. One of the two patients deveIoped the lesions during longterm antituberculosis chemotherapy for her advanced pulmonary tuberculosis, The other case developed the lesions, initially at the site of acupuncture done for relief of her arthralgia, followed by the involvement of the other sites. The etiology of acute febrile neutrophilic dermatosis is not clear, however, hypersensitivity and association with systemic diseases such as upper respiratory infection, malignancies, ulcerative colitis and pyoderma gangrenosum are postulated.
Acupuncture
;
Arthralgia
;
Colitis, Ulcerative
;
Drug Therapy
;
Female
;
Humans
;
Hypersensitivity
;
Pyoderma Gangrenosum
;
Sweet Syndrome*
;
Tuberculosis, Pulmonary
9.A clinical analysis of the appendicitis in children.
Tae Young JUNG ; Dae Hwa CHOI ; Chang Woo LEE
Journal of the Korean Surgical Society 1992;43(5):767-775
No abstract available.
Appendicitis*
;
Child*
;
Humans
10.Implication of Neoadjuvant Chemotherapy for the Management of Patients with Locally Advanced Cervical Cancer.
Jae Wook KIM ; Eun Gyung CHOI ; Young Tae KIM
Korean Journal of Obstetrics and Gynecology 2000;43(8):1375-1383
No abstract available.
Drug Therapy*
;
Humans
;
Uterine Cervical Neoplasms*