Alleles and genotype frequencies and its distribution pattern for the highly polymorphic D1S80 locus were determined in a Korean population sample, especially in Kwangju and Chonnam, by using PCR followed by agarose gel electrophoresis with ethidium bromide staining, a procedure called the amplified-fragment-length polymorphism(Amp-FLP) technique. And the data were compared with the alleles and genotype frequencies of Finnish population, North American Caucasian, and Korean population(Seoul) which had been reported. In 203 unrelated Korean individuals 27 alleles and 84 genotypes were observed. The highest allele frequency was in allele M24(0.128) and tne next orders were inalleles M18(0.126), M29, M30, M31, and M28 and the other alleles showed relatively low frequencies. The highest frequency of genotype was in M18/M24 and the next order frequencies were M18/M30, M19/M27 M29/M29, and M18/M29. The homozyous genotypes were in 9 alleles such as M29, M24, M31, and M18, and most of heterozygous genotypes were composed of alleles of each homozygous genotypes and /or the other alleles, its composition of genotypes was 0.881(74/84), 183(0.901) of the 203 individuals alleles, its composition of genotypes was 0.881(74/84), 183(0.901) of the 203 individuals alleles, its composition of genotypes was 0.881(74/84), 183(0.901) of the 203 individuals were included. The VNTR D1S80 locus demonstrated a heterozygosity of 0.872. From the above results, VNTR D1S80 locus may be a powerful locus to identify individuals, however, the allele frequencies was not closely related to the genotype pattern, and the alleles of homozygous genotypes influenced on the chance of the recombination of the various genotypes. It is necessary to analyze the genotype distribution and the recombination pattern of alleles as well as alleles and genotype frequencies in each populations for statistical test at most highly polymorphic loci.
Alleles* ; Electrophoresis, Agar Gel ; Ethidium ; Gene Frequency ; Genotype* ; Gwangju ; Jeollanam-do ; Polymerase Chain Reaction ; Recombination, Genetic

The authors present seven cases of Democidiosis in rosacea type and the subject of human demodetic infestation has been reviewed. According to our experience, demodex folliculorum, although a normal inhabitant of the sebaceous glands in small numbers, when encouraged to multiply by the patients excessive use of facial cream and avoidance of soap, can produce democidiosis. The diagnosis was easily confirmed by characteristic clinical appearance and simple microscopy. The characteristie clinical appearances are indurated non-comedous papules and pustules with unique cherry pinkish red erythema. The response to treatment, consisting of daily soap and water cleansing of the face, followed by the local application of a suitable antiparasitic remedies is simple and gratifying. But the erythema was not respond to antiparasitic preparation.
Diagnosis ; Erythema ; Humans ; Microscopy ; Prunus ; Rosacea* ; Sebaceous Glands ; Soaps ; Water

Herpes zoster is generally believed to be caused by the activation of varicella-zoster virus present in the body since an original infection with varicella. According to the thesis, the virus remairis in a latent state in the cells of the sensory ganglia until immunity has waned sufficiently to pezmit multiplication of the virus and clinical infection. There are a number of reports that in patient with malignant disease, especially Jymphoma, a frequenry af association of zoster is greater and severity of its symptom is increased to be marked contrast to the benign uneventful course of zoster seen in healthy person. The author observed one case of zoster seen in patient with malignant disorder, and frequency of association of zoster in malignant disease and its etiological factors are reviewed in the literature.
Chickenpox ; Ganglia, Sensory ; Herpes Zoster* ; Herpesvirus 3, Human ; Humans ; Lymphoma, Non-Hodgkin*

The authers observed one case of typical poikilodermatomyositis who was 47 year old female. The poikiIodermatomyositis is a rare disease which is a variant of dermatomyositis and has never been reported in Korea. This patient complained of a weakness of left lower leg and tingling sensation of both fingers since one year ago, And she has a difficulty of both finger flexion since 20 days ago. The skin lesions revealed mottled dark brownish pigmentation, hardness, scattered atrophic patches and telangiectasia in the lesion on the left lower leg since one year ago. The same skin changes appeared on the thigh, lower abdomen and upper chest on 4 months ago. Histopathologically, a biopsy was performed from the skin of lower leg, the skin shows typical poikiloderma, i.e. the epidermis shows moderate atrophy of the stratum malpighii, flattened rete ridges and hydropic degeneration of the hasal cells. The dermis is edematous and a band like dense lymphocytic cellular infiltration. And the muscIe biopsy from gastrocnemius muscle shows dermatomyositis finding which the muscle bundles are edematous and degenerative. Many inflammatory cells present between muscle bundles but there is no suppurative change. Diagnosis was confirmed by clinical appearance, histopathologically and other laboratory studies. The patient was markedly improved by the prednisolone 80 mg and vasculat (vascular dilatator) 100 mg daily.
Abdomen ; Atrophy ; Biopsy ; Bryophyta ; Dermatomyositis ; Dermis ; Diagnosis ; Epidermis ; Female ; Fingers ; Hardness ; Humans ; Korea ; Leg ; Middle Aged ; Muscle, Skeletal ; Pigmentation ; Prednisolone ; Rare Diseases ; Sensation ; Skin ; Telangiectasis ; Thigh ; Thorax

The authers observed one case of typical poikilodermatomyositis who was 47 year old female. The poikiIodermatomyositis is a rare disease which is a variant of dermatomyositis and has never been reported in Korea. This patient complained of a weakness of left lower leg and tingling sensation of both fingers since one year ago, And she has a difficulty of both finger flexion since 20 days ago. The skin lesions revealed mottled dark brownish pigmentation, hardness, scattered atrophic patches and telangiectasia in the lesion on the left lower leg since one year ago. The same skin changes appeared on the thigh, lower abdomen and upper chest on 4 months ago. Histopathologically, a biopsy was performed from the skin of lower leg, the skin shows typical poikiloderma, i.e. the epidermis shows moderate atrophy of the stratum malpighii, flattened rete ridges and hydropic degeneration of the hasal cells. The dermis is edematous and a band like dense lymphocytic cellular infiltration. And the muscIe biopsy from gastrocnemius muscle shows dermatomyositis finding which the muscle bundles are edematous and degenerative. Many inflammatory cells present between muscle bundles but there is no suppurative change. Diagnosis was confirmed by clinical appearance, histopathologically and other laboratory studies. The patient was markedly improved by the prednisolone 80 mg and vasculat (vascular dilatator) 100 mg daily.
Abdomen ; Atrophy ; Biopsy ; Bryophyta ; Dermatomyositis ; Dermis ; Diagnosis ; Epidermis ; Female ; Fingers ; Hardness ; Humans ; Korea ; Leg ; Middle Aged ; Muscle, Skeletal ; Pigmentation ; Prednisolone ; Rare Diseases ; Sensation ; Skin ; Telangiectasis ; Thigh ; Thorax

The nodular vasculitis described by Montgomery on 1945 and is characterized by relatively chronic, persistent, or recurrent nodular lesions of nontuberculous origin chiefly on the legs. In the differential diagnosis the following must also be considered; erythema induratum, eythema nodosum, Weber-Christian disease, erythema nodosum migrans, recurrent thrombophrebitis and periarteritis nodosa. The authors observed one case of nodular vasculitis caused by sulfa drug. This patient was diagnosed to papulonecrotic tuberculid at first and treated by prednisolone 20 mg, INH 300 mg and streptomycin l.0 gm BIW. By the treatment, the patient, was cured completly within 3 months but visited again because of recurrence after 6 months. Therefore, same medication was given, but did not show improvment and added sulfa drug, Lederkyne. But, unfortunately the skin lesions was aggrevated after sulfa medication. It was suggestive that the cause of aggrevation was sulfa drug and discontinued the sulfa drug. The skin lesions were completely cured after the drug was discontinued.
Diagnosis, Differential ; Erythema Induratum ; Erythema Nodosum ; Humans ; Leg ; Panniculitis, Nodular Nonsuppurative ; Polyarteritis Nodosa ; Prednisolone ; Recurrence ; Skin ; Streptomycin ; Tuberculosis, Cutaneous* ; Vasculitis*

No abstract available.
Child* ; Diverticulum* ; Humans

No abstract available.
T-Lymphocytes*

Recently, the number of cataract surgeries has rapidly increased. This study was devised to evaluate the post-operative daily life behavior and degree of satisfaction in the patients who underwent cataract surgery two or more years ago. Of 400 patients who underwent ECCE with IOL implantation, 125 patients[31%]replied to the mail questionnaire consisted of thirty questions. Hospital records were also reviewed. 84%of the patients were satisfied with outcomes of cataract surgery, which was significantly correlated with current visual acuity[p<0.01]. Degree of satisfaction was significantly higher in patients with bilateral surgery compared with those with unilateral surgery[p<0.05]. Age, sex, occupation, economic and general health status were not significantly associated with degree of satisfaction. As for life behavior, discomfort while driving an automobile was the most common complaint, especially in the patients who underwent unilateral surgery. Concerning ocular symptoms, many patients complained of foreign body sense, epiphora, glare, fatigue, and near vision discomfort. We reconfirmed that the decision to perform cataract surgery should be made with careful consideration of long term prognosis and above results.
Automobiles ; Cataract* ; Fatigue ; Foreign Bodies ; Glare ; Hospital Records ; Humans ; Lacrimal Apparatus Diseases ; Occupations ; Postal Service ; Prognosis ; Surveys and Questionnaires

Many investigators were interested in the pathogenesis and the relationship between microscopical features and clinical behavior of hydatidiform mole. Trophoblastic cells in the trophoblastic disease were intensively examined histologically, ultrastructurally, immunohistochemically, and with hormone assay method, etc. But ultrastructural study on the stroma of hydatidiform mole was scarcely reported. In this paper, hydatidiform mole was examined at light and electron microscopic levels, with emphasis on the stroma. The results were as follows: 1) Hydropic degeneration of H-mole is more severe in the center of stroma and is not related with the degree of trophoblastic proliferation. Hofbauer cell and vascular structure are extremely rarely observed in the periphery of stroma which has relatively preserved cellular components. 2) Basement membrane is sometimes separated from trophoblastic layer. Degenerated cells in the stroma contain vacuoles, autophagosomes, and lipid droplets. Collagen is abundant in the loose interstitium. Hofbauer cells have no lysosome or phagosome. Vascular lumen is patient and endothelial cells are degenerated. From the above results, H-mole may be produced due to abnormal changes of trophoblasts and stromal changes may be a secondary process, so called autolysis. Hofbauer cells are not engaged in the stromal degeneration and may be different from usual tissue macrophages.