1.Transurethral Prostatectomy for the Patients Over 80 Years Old : Is It Safe?.
Tae Kyoon NA ; Dae Kyung KIM ; Tag Keun YOO
Korean Journal of Urology 2000;41(9):1086-1090
No abstract available.
Aged, 80 and over*
;
Humans
;
Transurethral Resection of Prostate*
2.Metanephric Adenoma of the Kidney: A Case Report.
Tae Kyoon NA ; Jae Hoon HOH ; Byung Goo YEO ; Dae Kyung KIM ; Ki Hwoa YANG ; Jin Young JUNG ; Hyeon JEONG
Korean Journal of Urology 2000;41(6):781-784
3.Metanephric Adenoma of the Kidney: A Case Report.
Tae Kyoon NA ; Jae Hoon HOH ; Byung Goo YEO ; Dae Kyung KIM ; Ki Hwoa YANG ; Jin Young JUNG ; Hyeon JEONG
Korean Journal of Urology 2000;41(6):781-784
4.A Case of Bilateral Hutch's Diverticular Associated with Vesicoureteral Reflux.
Tae Kyoon NA ; Gyu Young YEUM ; Jae Hun NOH ; Byung Goo YEO ; Hyun JUNG ; Dae Kyung KIM
Korean Journal of Urology 2000;41(9):1144-1146
No abstract available.
Vesico-Ureteral Reflux*
5.Repeated Glucose Deprivation/Reperfusion Induced PC-12 Cell Death through the Involvement of FOXO Transcription Factor.
Na HAN ; You Jeong KIM ; Su Min PARK ; Seung Man KIM ; Ji Suk LEE ; Hye Sook JUNG ; Eun Ju LEE ; Tae Kyoon KIM ; Tae Nyun KIM ; Min Jeong KWON ; Soon Hee LEE ; Mi kyung KIM ; Byoung Doo RHEE ; Jeong Hyun PARK
Diabetes & Metabolism Journal 2016;40(5):396-405
BACKGROUND: Cognitive impairment and brain damage in diabetes is suggested to be associated with hypoglycemia. The mechanisms of hypoglycemia-induced neural death and apoptosis are not clear and reperfusion injury may be involved. Recent studies show that glucose deprivation/reperfusion induced more neuronal cell death than glucose deprivation itself. The forkhead box O (FOXO) transcription factors are implicated in the regulation of cell apoptosis and survival, but their role in neuronal cells remains unclear. We examined the role of FOXO transcription factors and the involvement of the phosphatidylinositol 3-kinase (PI3K)/Akt and apoptosis-related signaling pathways in PC-12 cells exposed to repeated glucose deprivation/reperfusion. METHODS: PC-12 cells were exposed to control (Dulbecco's Modified Eagle Medium [DMEM] containing 25 mM glucose) or glucose deprivation/reperfusion (DMEM with 0 mM glucose for 6 hours and then DMEM with 25 mM glucose for 18 hours) for 5 days. MTT assay and Western blot analysis were performed for cell viability, apoptosis, and the expression of survival signaling pathways. FOXO3/4',6-diamidino-2-phenylindole staining was done to ascertain the involvement of FOXO transcription factors in glucose deprivation/reperfusion conditions. RESULTS: Compared to PC-12 cells not exposed to hypoglycemia, cells exposed to glucose deprivation/reperfusion showed a reduction of cell viability, decreased expression of phosphorylated Akt and Bcl-2, and an increase of cleaved caspase-3 expression. Of note, FOXO3 protein was localized in the nuclei of glucose deprivation/reperfusion cells but not in the control cells. CONCLUSION: Repeated glucose deprivation/reperfusion caused the neuronal cell death. Activated FOXO3 via the PI3K/Akt pathway in repeated glucose deprivation/reperfusion was involved in genes related to apoptosis.
Apoptosis
;
Blotting, Western
;
Brain
;
Caspase 3
;
Cell Death*
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Cell Survival
;
Cognition Disorders
;
Eagles
;
Glucose*
;
Hypoglycemia
;
Neurons
;
Phosphatidylinositol 3-Kinase
;
Reperfusion
;
Reperfusion Injury
;
Transcription Factors*
6.Co-Culture of alpha TC-6 Cells and beta TC-1 Cells: Morphology and Function.
Sung Man KIM ; Eun Ju LEE ; Hye Sook JUNG ; Na HAN ; You Jeong KIM ; Tae Kyoon KIM ; Tae Nyun KIM ; Min Jeong KWON ; Soon Hee LEE ; Jeong Hyun PARK ; Byoung Doo RHEE ; Mi Kyung KIM
Endocrinology and Metabolism 2015;30(1):92-97
BACKGROUND: In vitro experiments using only beta-cell lines instead of islets are limited because pancreatic islets are composed of four different types of endocrine cells. Several recent studies have focused on cellular interactions among these cell types, especially alpha- and beta-cells. Because islet isolation needs time and experience, we tested a simple co-culture system with alpha- and beta-cells. Their morphology and function were assessed by comparison to each single cell culture and pancreatic islets. METHODS: alpha TC-6 cells and beta TC-1 cells were maintained in Dulbecco's Minimal Essential Medium containing 5 mM glucose and 10% fetal bovine serum. Cells were mixed at a 1:1 ratio (5x10(5)) in 6-well plates and cultured for 24, 48, and 72 hours. After culture, cells were used for insulin and glucagon immunoassays and tested for glucose-stimulated insulin secretion (GSIS). RESULTS: alpha TC-6 and beta TC-1 cells became condensed by 24 hours and were more strongly compacted after 48 hours. beta TC-1 cells showed both beta-beta and beta-alpha cell contacts. GSIS increased with increasing glucose concentration in co-cultured cells, which showed lower secreted insulin levels than beta TC-1 cells alone. The increase in the secreted insulin/insulin content ratio was significantly lower for co-cultured cells than for beta-cells alone (P=0.04). Compared to islets, the alpha-/beta-cell co-culture showed a higher ratio of GSIS to insulin content, but the difference was not statistically significant (P=0.09). CONCLUSION: alpha TC-6 and beta TC-1 cells in the co-culture system showed cell-to-cell contacts and a similar stimulated insulin secretion pattern to islets. The co-culture system may be used to better mimic pancreatic islets in in vitro assessments.
Cell Culture Techniques
;
Coculture Techniques*
;
Endocrine Cells
;
Glucagon
;
Glucose
;
Immunoassay
;
Insulin
;
Islets of Langerhans